Plant genetics and biotechnology represent the frontiers of modern agricultural and biological sciences, having given us the green revolution and the oilseeds 'yellow' revolution. The new science, this gene revolution, produced the high-yielding wheat, rice, maize and barley seeds that brought about a revolutionary change in the world's food supplies, from a state of perennial shortages to abundance and even surplus. Plant genetics is different from that of animals in a few ways. Like mitochondria, chloroplasts have their own DNA, complicating pedigrees somewhat. Like animals, plants have somatic mutations regularly, but these mutations can contribute to the germ line with ease, since flowers develop at the ends of branches composed of somatic cells. People have known of this for centuries, and mutant branches are called "e;sports"e;. If the fruit on the sport is economically desirable, a new cultivar may be obtained. Plant Biotechnology has made tremendous progress in recent years. The roots of plant biotechnology can be traced back to the time when humans started collecting seeds from their favorite wild plants and began cultivating them in tended fields. It appears that when the plants were harvested, the seeds of the most desirable plants were retained and replanted the next growing season. The present publication aims to provide enough information and examples to give the reader a sound knowledge of plant biotechnology in all its guises. This book explores many vistas of biotechnology, which will be useful for scientists and students to understand the latest techniques and processes of biotechnology and plant genetics.
Advancements in molecular and cell biology have led to the development of a range of techniques for manipulating genomes, collectively termed as biotechnology. Today, biotechnology is being used as a tool to give plants new traits that benefit agricultural production, the environment, and human nutrition and health. Modern techniques of genetic engineering are essentially a refinement of the kinds of genetic modifications that have long been used to enhance plants, microorganisms, and animals for food. The products of the newer techniques are even more predictable and safer than the genetically engineered foods that have long enriched our diet.
Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr VIGS) Virus-based microRNA silencing (VbMS) and Virus-based RNA mobility assays (VRMA); RNA based vaccines and expression of virus proteins or RNA, and virus-like particles in plants, the potential of virus vaccines and therapeutics, and exploring plants as factories for useful products and pharmaceuticals are topics wholly deepened. The book reviews and discuss Plant Functional Genomic studies discussing the technologies supporting the genetic improvement of plants and the production of plant varieties more resistant to biotic and abiotic stresses. Several important crops are analysed providing a glimpse on the most up-to-date methods and topics of investigation. The book presents a review on current state of GMO, the cisgenesis-derived plants and novel plant products devoid of transgene elements, discuss their regulation and the production of desired traits such as resistance to viruses and disease also in fruit trees and wood trees with long vegetative periods. Several chapters cover aspects of plant physiology related to plant improvement: cytokinin metabolism and hormone signaling pathways are discussed in barley; PARP-domain proteins involved in Stress-Induced Morphogenetic Response, regulation of NAD signaling and ROS dependent synthesis of anthocyanins. Apple allergen isoforms and the various content in different varieties are discussed and approaches to reduce their presence. Euphorbiaceae, castor bean, cassava and Jathropa are discussed at genomic structure, their diseases and viruses, and methods of transformation. Rice genomics and agricultural traits are discussed, and biotechnology for engineering and improve rice varieties. Mango topics are presented with an overview of molecular methods for variety differentiation, and aspects of fruit improvement by traditional and biotechnology methods. Oilseed rape is presented, discussing the genetic diversity, quality traits, genetic maps, genomic selection and comparative genomics for improvement of varieties. Tomato studies are presented, with an overview on the knowledge of the regulatory networks involved in flowering, methods applied to study the tomato genome-wide DNA methylation, its regulation by small RNAs, microRNA-dependent control of transcription factors expression, the development and ripening processes in tomato, genomic studies and fruit modelling to establish fleshy fruit traits of interest; the gene reprogramming during fruit ripening, and the ethylene dependent and independent DNA methylation changes. - provides an overview on the ongoing projects and activities in the field of applied biotechnology - includes examples of different crops and applications to be exploited - reviews and discusses Plant Functional Genomic studies and the future developments in the field - explores the new technologies supporting the genetic improvement of plants
Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
As the oldest and largest human intervention in nature, the science of agriculture is one of the most intensely studied practices. From manipulation of plant gene structure to the use of plants for bioenergy, biotechnology interventions in plant and agricultural science have been rapidly developing over the past ten years with immense forward leaps on an annual basis. This book begins by laying the foundations for plant biotechnology by outlining the biological aspects including gene structure and expression, and the basic procedures in plant biotechnology of genomics, metabolomics, transcriptomics and proteomics. It then focuses on a discussion of the impacts of biotechnology on plant breeding technologies and germplasm sustainability. The role of biotechnology in the improvement of agricultural traits, production of industrial products and pharmaceuticals as well as biomaterials and biomass provide a historical perspective and a look to the future. Sections addressing intellectual property rights and sociological and food safety issues round out the holistic discussion of this important topic. Includes specific emphasis on the inter-relationships between basic plant biotechnologies and applied agricultural applications, and the way they contribute to each other Provides an updated review of the major plant biotechnology procedures and techniques, their impact on novel agricultural development and crop plant improvement Takes a broad view of the topic with discussions of practices in many countries
Applied Molecular Biotechnology: The Next Generation of Genetic Engineering explains state-of-the-art advances in the rapidly developing area of molecular biotechnology, the technology of the new millennium. Comprised of chapters authored by leading experts in their respective fields, this authoritative reference text:Highlights the latest omics-ba
Biotechnology and Plant Breeding includes critical discussions of the newest and most important applications of biotechnology in plant breeding, covering key topics such as biometry applied to molecular analysis of genetic diversity, genetically modified plants, and more. This work goes beyond recombinant DNA technology to bring together key information and references on new biotech tools for cultivar development, such as double-haploids, molecular markers, and genome-wide selection, among others. It is increasingly challenging for plant breeders and agricultural systems to supply enough food, feed, fiber and biofuel for the global population. As plant breeding evolves and becomes increasingly sophisticated, a staggering volume of genetic data is now generated. Biotechnology and Plant Breeding helps researchers and students become familiar with how the vast amounts of genetic data are generated, stored, analyzed and applied. This practical resource integrates information about plant breeding into the context of modern science, and assists with training for plant breeders including those scientists who have a good understanding of molecular biology/biotechnology and need to learn the art and practice of plant breeding. Plant biologists, breeding technicians, agronomists, seed technologists, students, and any researcher interested in biotechnologies applied to plant breeding will find this work an essential tool and reference for the field. - Presents in-depth but easy-to-understand coverage of topics, so plant breeders can readily comprehend them and apply them to their breeding programs - Includes chapters that address the already developed and optimized biotechnologies for cultivar development, with real-world application for users - Features contributions by authors with several years of experience in their areas of expertise
