Applications of Hydrophilic Interation Chromatography in the Analysis of Drugs and Metabolites
Author: Saud Bawazeer
Publisher:
Published: 2012
Total Pages:
ISBN-13:
DOWNLOAD EBOOKPosts
A Study of the Mechanism of Hydrophilic Interaction Chromatography and Its Application in the Analysis of Medical Drugs and Metabolites
Author: Eman Yousef Santali
Publisher:
Published: 2017
Total Pages: 628
ISBN-13:
DOWNLOAD EBOOKHydrophilic interaction chromatography (HILIC) has been the subject of a few excellent reviews in recent years and most focused on different factors which impact on the separation of compounds in HILIC mode including column temperature, mobile phase composition, pH, buffer type and concentration. However, there have not been as many studies focusing on the retention mechanism and selectivity in hydrophilic chromatography. In the light of recent development in HILIC stationary phases and their applications, the need to understand the mechanisms that govern the separation in HILIC, which is not purely due to partitioning, and the contribution of stationary surface became a subject of study. This thesis explored the possible mechanisms which might be involved in HILIC chromatography by comparing the retention of test probes on bare silica gel and on a type-C silica hydride phase. In addition, a comprehensive retention and selectivity study of some commercially available hydride-based stationary phases was carried out in the analysis of hydrophobic acids and bases in hydrophilic interaction chromatography (HILIC). The applications of HILIC technique were also addressed in the analysis of plasma and urinary metabolites, impurity profiling and in the separation of ‘Legal high’ regioisomers. An interest in the HILIC retention mechanism was mainly inspired by the need to understand how to develop the optimal HILIC conditions and the selection of a stationary phase. The retention of a series of positively charged probes on silica gel column was concluded that for this class of compounds both ion-exchange and hydrophilic partitioning were involved in retention. Ion exchange interactions were minimised by increase the ionic strength of the modifier in the mobile phase. The strength of the overall HILIC mechanism was increased with silica gel surface area. The unique properties of silica-hydride phases also assessed and are still not explained in terms of how the retention mechanism of this type of stationary phase is different from the other separation materials used in chromatography. The study confirmed the usefulness of HILIC in many analytical aspects. However, the mixed-interaction mechanisms which operate on different stationary phases for different compounds are still far from understood and more investigation is required.
Hydrophilic Interaction Chromatography
Author: Bernard A. Olsen
Publisher: John Wiley & Sons
Published: 2012-12-14
Total Pages: 336
ISBN-13: 1118495233
DOWNLOAD EBOOKDiscover how to use HILIC to analyze and better understand polar compounds An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications. Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring: Detailed examples of HILIC methods and development approaches Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods References to the primary literature at the end of each chapter Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.
Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications
Author: Perry G. Wang
Publisher: CRC Press
Published: 2011-02-17
Total Pages: 613
ISBN-13: 1439807531
DOWNLOAD EBOOKThis is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to proteins, the text explores the many applications of HILIC in the analytical field. Winner of the President's Award for Excellence, the author explains how HILIC can significantly improve analytical throughput by shortening sample preparation procedure, which is one of the bottlenecks for drug discovery and development in the pharmaceutical industry.
The Combination of Hydrophilic Interaction Liquid Chromatography and High Resolution Mass Spectrometry (HILIC-HRMS)
Author: Muhammad Anas Kamleh
Publisher:
Published: 2010
Total Pages: 0
ISBN-13:
DOWNLOAD EBOOKDifferential Ion Mobility Spectrometry
Author: Alexandre A. Shvartsburg
Publisher: CRC Press
Published: 2008-12-24
Total Pages: 322
ISBN-13: 9781420051070
DOWNLOAD EBOOKOver the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.
Hydrophilic Interaction Chromatography
Author: Bernard A. Olsen
Publisher: John Wiley & Sons
Published: 2013-01-22
Total Pages: 336
ISBN-13: 1118054172
DOWNLOAD EBOOKDiscover how to use HILIC to analyze and better understand polar compounds An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications. Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring: Detailed examples of HILIC methods and development approaches Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods References to the primary literature at the end of each chapter Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.
HPLC for Pharmaceutical Scientists
Author: Yuri V. Kazakevich
Publisher: John Wiley & Sons
Published: 2007-02-16
Total Pages: 1136
ISBN-13: 0470087943
DOWNLOAD EBOOKHPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.
Thin Layer Chromatography in Drug Analysis
Author: Lukasz Komsta
Publisher: CRC Press
Published: 2013-12-20
Total Pages: 1067
ISBN-13: 1466507160
DOWNLOAD EBOOKUsed routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date
Chromatography
Author: E. Heftmann
Publisher: Elsevier
Published: 2004-04-16
Total Pages: 706
ISBN-13: 0080472257
DOWNLOAD EBOOKChromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis. Part B of this two volume set brings the traditional field of application up to date. These include amino acids and proteins, nucleic acids and their constituents, lipid, and carbohydrates. Special chapters are devoted to the most important areas of application: drug and environmental analysis. Forensic and phytochemical applications are covered for the first time. Together with an overview of computer resources, the subject index allows novices as well as experts to obtain rapid and authoritative guidance to analytical problems, such as choice of methods and optimization of techniques and instrumentation.1. Each chapter written by an authority 2. Thorough treatment of the theoretical basis of separation methods 3. Practical guide for performing analyses
