The Resolution Revolution: Recent Advances In cryoEM
The Resolution Revolution: Recent Advances In cryoEM

Author:

Publisher: Academic Press

Published: 2016-08-26

Total Pages: 488

ISBN-13: 0128054352

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cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods that determine the structures of biological molecules, a vital step for understanding their function - Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted

Enzyme Kinetics
Enzyme Kinetics

Author: Alejandro G. Marangoni

Publisher: John Wiley & Sons

Published: 2003-04-23

Total Pages: 247

ISBN-13: 0471461415

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Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.

Enzyme Regulation in Metabolic Pathways
Enzyme Regulation in Metabolic Pathways

Author: Lloyd Wolfinbarger, Jr.

Publisher: John Wiley & Sons

Published: 2017-02-03

Total Pages: 226

ISBN-13: 111915541X

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Enzyme Regulation in Metabolic Pathways shows the reader how to understand the roles of enzymes and their kinetic constants in intermediary metabolism. It provides a means of correlating data obtained in experimental studies to multiple possible mechanisms through which some enzyme may catalyze the conversion of a substrate to a product. Although not the most appropriate means of determining some potential kinetic mechanism, quasi-equilibrium assumptions are used throughout the book, keeping the rate equation derivations simple. Actual metabolic pathways with known (presumed) positive and negative regulation events are linked to these potential kinetic mechanisms using both rate equation derivations and data plots illustrating how the rate equation derivations can be used to explain the data plots. This book will be a valuable reference for students in biological sciences and biochemistry majors required to take a core course in enzymology.

Directed Enzyme Evolution: Advances and Applications
Directed Enzyme Evolution: Advances and Applications

Author: Miguel Alcalde

Publisher: Springer

Published: 2017-02-14

Total Pages: 286

ISBN-13: 3319504134

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This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art. Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest can now be engineered in a manner that surpasses the boundaries of nature. The volume is divided into two sections, the first of which provides an update on recent successful cases of enzyme ensembles from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the book summarizes a variety of the most applicable methods for library creation, together with the future trends aimed at bringing together directed evolution and in silico/computational enzyme design and ancestral resurrection.

Analytical Techniques in Biochemistry and Molecular Biology
Analytical Techniques in Biochemistry and Molecular Biology

Author: Rajan Katoch

Publisher: Springer Science & Business Media

Published: 2011-07-19

Total Pages: 449

ISBN-13: 1441997857

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Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Proteins, Enzymes, Genes
Proteins, Enzymes, Genes

Author:

Publisher: Yale University Press

Published:

Total Pages: 814

ISBN-13: 9780300153590

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In this book a distinguished scientist-historian offers a critical account of how biochemistry and molecular biology emerged as major scientific disciplines from the interplay of chemical and biological ideas and practice. Joseph S. Fruton traces the historical development of these disciplines from antiquity to the present time, examines their institutional settings, and discusses their impact on medical, pharmaceutical, and agricultural practice.

Practical Enzymology
Practical Enzymology

Author: Hans Bisswanger

Publisher: John Wiley & Sons

Published: 2019-11-06

Total Pages: 574

ISBN-13: 3527820809

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A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.