Advanced Time-Correlated Single Photon Counting Techniques

Advanced Time-Correlated Single Photon Counting Techniques

Author: Wolfgang Becker

Publisher: Springer Science & Business Media

Published: 2005-12-19

Total Pages: 414

ISBN-13: 3540288821

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In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.


Advanced Photon Counting

Advanced Photon Counting

Author: Peter Kapusta

Publisher: Springer

Published: 2015-04-23

Total Pages: 371

ISBN-13: 3319156365

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This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.


Time-correlated single photon counting

Time-correlated single photon counting

Author: Desmond O'Connor

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 299

ISBN-13: 0323141447

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Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.


Handbook for Highly Charged Ion Spectroscopic Research

Handbook for Highly Charged Ion Spectroscopic Research

Author: Yaming Zou

Publisher: Taylor & Francis

Published: 2016-04-19

Total Pages: 452

ISBN-13: 1420079050

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The spectroscopy of highly charged ions plays a key role in numerous areas of physics, from quantum electrodynamics (QED) and parity nonconservation (PNC) testing to fusion and plasma physics to x-ray astronomy. Handbook for Highly Charged Ion Spectroscopic Research brings together many of the techniques and ideas needed to carry out state-of-the-a


Spectroscopy and Dynamics of Single Molecules

Spectroscopy and Dynamics of Single Molecules

Author:

Publisher: Elsevier

Published: 2019-08-14

Total Pages: 404

ISBN-13: 0128164646

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Spectroscopy and Dynamics of Single Molecules: Methods and Applications reviews the most recent developments in spectroscopic methods and applications. Spectroscopic techniques are the chief experimental methods for testing theoretical models and research in this area plays an important role in stimulating new theoretical developments in physical chemistry. This book provides an authoritative insight into the latest advances in the field, highlighting new techniques, current applications, and potential future developments An ideal reference for chemists and physicists alike, Spectroscopy and Dynamics of Single Molecules: Methods and Applications is a useful guide for all those working in the research, design, or application of spectroscopic tools and techniques across a wide range of fields. - Includes the latest research on ultrafast vibrational and electronic dynamics, nonlinear spectroscopies, and single-molecule methods - Makes the content accessible to researchers in chemistry, biophysics, and chemical physics through a rigorous multi-disciplinary approach - Provides content edited by a world-renowned chemist with more than 30 years of experience in research and instruction


Quantum Photonics: Pioneering Advances and Emerging Applications

Quantum Photonics: Pioneering Advances and Emerging Applications

Author: Robert W. Boyd

Publisher: Springer

Published: 2019-02-19

Total Pages: 646

ISBN-13: 3319984020

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This book brings together reviews by internationally renowed experts on quantum optics and photonics. It describes novel experiments at the limit of single photons, and presents advances in this emerging research area. It also includes reprints and historical descriptions of some of the first pioneering experiments at a single-photon level and nonlinear optics, performed before the inception of lasers and modern light detectors, often with the human eye serving as a single-photon detector. The book comprises 19 chapters, 10 of which describe modern quantum photonics results, including single-photon sources, direct measurement of the photon's spatial wave function, nonlinear interactions and non-classical light, nanophotonics for room-temperature single-photon sources, time-multiplexed methods for optical quantum information processing, the role of photon statistics in visual perception, light-by-light coherent control using metamaterials, nonlinear nanoplasmonics, nonlinear polarization optics, and ultrafast nonlinear optics in the mid-infrared.


Advances in Brain Imaging Techniques

Advances in Brain Imaging Techniques

Author: Nirmal Mazumder

Publisher: Springer Nature

Published: 2022-05-19

Total Pages: 265

ISBN-13: 9811913528

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The book reviews the recent developments in brain imaging and their technological advancements to understand molecular mechanisms associated with neurological disorders and basic behaviors in humans and rodents at the structural, molecular, and functional levels. It discusses the usefulness of advanced optical microscopy techniques, including optical coherence tomography (OCT), miniscope, multiphoton fluorescence (2PF & 3PF), adaptive optics, harmonic generation, and Raman microscopy for understanding pathomechanism of brain disorders and pathological and physiological changes associated with neurodegenerative diseases. Also, the book presents conventional imaging modalities, including Magnetic Resonance Imaging (MRI), for delineating underlying mechanisms and precise early diagnosis of neurological disorders. This book is a useful resource for neuroscientists and researchers working in biomedical engineering and optics.


Single-Photon Generation and Detection

Single-Photon Generation and Detection

Author: Sergio Cova

Publisher: Elsevier Inc. Chapters

Published: 2013-11-29

Total Pages: 76

ISBN-13: 012805803X

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This chapter presents a broad review of the principle, design, fabrication, operation, and performance of single-photon avalanche diodes (SPADs), and provides a comparative basis to assess the relative merits of the wide variety of devices and techniques covered. Particular attention is paid to the design of and operation of both silicon and high-speed-gated InGaAs/InP detection systems, as well as array-based systems.


Biomedical Photonics Handbook, 3 Volume Set

Biomedical Photonics Handbook, 3 Volume Set

Author: Tuan Vo-Dinh

Publisher: CRC Press

Published: 2014-07-29

Total Pages: 2465

ISBN-13: 1439863199

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This handbook presents the most recent technological advances and applications in the areas of biomedical photonics. This second edition contains introductory material and covers the state-of-the-art methods and instrumentation for biomedical photonic technologies. It integrates interdisciplinary research and development critically needed for scientists, engineers, manufacturers, teachers, students, and clinical providers to learn about the most recent advances and predicted trends in instrumentation and methods as well as clinical applications in important areas of biomedical photonics. Extensive references are provided to enhance further study.


ISTFA 2013

ISTFA 2013

Author: A. S. M. International

Publisher: ASM International

Published: 2013-01-01

Total Pages: 634

ISBN-13: 1627080228

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This volume features the latest research and practical data from the premier event for the microelectronics failure analysis community. The papers cover a wide range of testing and failure analysis topics of practical value to anyone working to detect, understand, and eliminate electronic device and system failures.