Advanced Flow Cytometry: Applications in Biological Research
Advanced Flow Cytometry: Applications in Biological Research

Author: R.C. Sobti

Publisher: Springer Science & Business Media

Published: 2013-04-17

Total Pages: 152

ISBN-13: 9401706239

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Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.

Flow Cytometry
Flow Cytometry

Author: Marion G. Macey

Publisher: Springer Science & Business Media

Published: 2007-11-03

Total Pages: 289

ISBN-13: 1597454516

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Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Advanced Micro- and Nano-manufacturing Technologies
Advanced Micro- and Nano-manufacturing Technologies

Author: Shrikrishna Nandkishor Joshi

Publisher: Springer Nature

Published: 2021-10-01

Total Pages: 404

ISBN-13: 9811636451

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This volume focuses on the fundamentals and advancements in micro and nanomanufacturing technologies applied in the biomedical and biochemical domain. The contents of this volume provide comprehensive coverage of the physical principles of advanced manufacturing technologies and the know-how of their applications in the fabrication of biomedical devices and systems. The book begins by documenting the journey of miniaturization and micro-and nano-fabrication. It then delves into the fundamentals of various advanced technologies such as micro-wire moulding, 3D printing, lithography, imprinting, direct laser machining, and laser-induced plasma-assisted machining. It also covers laser-based technologies which are a promising option due to their flexibility, ease in control and application, high precision, and availability. These technologies can be employed to process several materials such as glass, polymers: polycarbonate, polydimethylsiloxane, polymethylmethacrylate, and metals such as stainless steel, which are commonly used in the fabrication of biomedical devices, such as microfluidic technology, optical and fiber-optic sensors, and electro-chemical bio-sensors. It also discusses advancements in various MEMS/NEMS based technologies and their applications in energy conversion and storage devices. The chapters are written by experts from the fields of micro- and nano-manufacturing, materials engineering, nano-biotechnology, and end-users such as clinicians, engineers, academicians of interdisciplinary background. This book will be a useful guide for academia and industry alike.

Flow Cytometry
Flow Cytometry

Author: Alice Longobardi Givan

Publisher: John Wiley & Sons

Published: 2013-04-10

Total Pages: 309

ISBN-13: 1118688392

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Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Advanced Optical Flow Cytometry
Advanced Optical Flow Cytometry

Author: Valery V. Tuchin

Publisher: John Wiley & Sons

Published: 2011-03-31

Total Pages: 698

ISBN-13: 3527634290

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A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.

Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications
Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications

Author: Neetu Mishra

Publisher: Bentham Science Publishers

Published: 2019-10-30

Total Pages: 153

ISBN-13: 9811422850

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Advances in biomedical research have had a profound effect on human health outcomes over the last century. Biophysical, biochemical and cellular techniques are now the backbone of modern biomedical research. Understanding these laboratory techniques is a prerequisite for investigating the processes responsible for human diseases and discovering new treatment methods. Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications Provides information about basic and advanced analytical techniques applied in specific areas of life science and biomedical Key Features: - Book chapters present a broad overview of sophisticated analytical techniques used in biophysics, biochemistry and cell biology. - Techniques covered include in vitro cell culture techniques, flow cytometry, real time PCR, X-ray crystallography, RNA sequencing - Information about industrial and biomedical applications of techniques, (drug screening, disease models, functional assays, disease diagnosis, gene expression analysis and protein structure determination) is included. The book is an excellent introduction for students (as a textbook) and researchers (as a reference work). The information it presents will prepare readers to understand and develop research methods in life science laboratories for different projects and activities.

Flow Cytometry with Plant Cells
Flow Cytometry with Plant Cells

Author: Jaroslav Dolezel

Publisher: John Wiley & Sons

Published: 2007-06-27

Total Pages: 479

ISBN-13: 3527610936

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Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.

Omics Technologies and Bio-engineering
Omics Technologies and Bio-engineering

Author: Debmalya Barh

Publisher: Academic Press

Published: 2017-12-01

Total Pages: 645

ISBN-13: 0128047496

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Omics Technologies and Bio-Engineering: Towards Improving Quality of Life, Volume 1 is a unique reference that brings together multiple perspectives on omics research, providing in-depth analysis and insights from an international team of authors. The book delivers pivotal information that will inform and improve medical and biological research by helping readers gain more direct access to analytic data, an increased understanding on data evaluation, and a comprehensive picture on how to use omics data in molecular biology, biotechnology and human health care. - Covers various aspects of biotechnology and bio-engineering using omics technologies - Focuses on the latest developments in the field, including biofuel technologies - Provides key insights into omics approaches in personalized and precision medicine - Provides a complete picture on how one can utilize omics data in molecular biology, biotechnology and human health care

Flow Cytometry Basics for the Non-Expert
Flow Cytometry Basics for the Non-Expert

Author: Christine Goetz

Publisher: Springer

Published: 2018-11-08

Total Pages: 229

ISBN-13: 3319980718

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This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.