Troubleshooting and Problem-Solving in the IVF Laboratory

Troubleshooting and Problem-Solving in the IVF Laboratory

Author: Kay Elder

Publisher: Cambridge University Press

Published: 2015-06-18

Total Pages: 229

ISBN-13: 1316301214

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Maintaining consistent and reliably high success rates is a daily challenge for every IVF laboratory. This step-by-step guide is an essential aid in navigating the complex maze of physical, chemical, biological, and logistic parameters that underpin successful gamete and embryo culture: temperature, pH, osmolality, gas supplies, air quality, light exposure, infections, managing supplies, personnel, as well as overall quality control. Numerous real-life troubleshooting case reports are presented, identifying all aspects necessary for troubleshooting. Process maps and flow charts accompanying each chapter offer a logical and systematic approach to problem solving in the laboratory. This is an essential resource for scientists in assisted reproductive technology and specialists in reproductive biology and medicine, helping IVF clinics to achieve the dream of every infertile couple: the birth of a healthy child.


Assisted Reproduction Techniques

Assisted Reproduction Techniques

Author: Khaldoun Sharif

Publisher: John Wiley & Sons

Published: 2012-03-26

Total Pages: 473

ISBN-13: 1444335553

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Assisted reproduction techniques have led to the birth of 4 million babies worldwide Assisted reproduction techniques (ART), in particular in-vitro fertilization and intra-cytoplasmic sperm injection, are the most advanced forms of infertility treatment. They involve numerous counseling, medical, surgical and laboratory-based steps. At each step various problems and complications could be encountered that challenge even the most experienced ART practitioners. Moreover, patients with complex medical disorders may require ART, presenting further challenges. Assisted Reproduction Techniques will stimulate resourceful thinking in the ART practitioner when faced with these challenges. It outlines various management options, the reasoning behind them, and the evidence on which they are based to enable the practitioner to choose the most suitable solution for the needs of each patient. Written by 122 internationally renowned experts, Assisted Reproduction Techniques follows the patient's journey throughout the whole ART process, with chapters on: Counseling and preparation Ovarian stimulation Oocyte retrieval Embryo transfer The luteal phase The ART laboratory The male patient The ART pregnancy Each of the 100 concise chapters includes clinical cases, background, evidence-based practical management options, preventive measures and key-point summaries of the important details. Assisted Reproduction Techniques gives a wide-ranging practical guide to all those wishing to support couples who cannot conceive naturally.


In Vitro Fertilization

In Vitro Fertilization

Author: Zsolt Peter Nagy

Publisher: Springer

Published: 2019-07-03

Total Pages: 926

ISBN-13: 3319430114

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Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.


Atlas of Oocytes, Zygotes and Embryos in Reproductive Medicine

Atlas of Oocytes, Zygotes and Embryos in Reproductive Medicine

Author: Marc Van den Bergh

Publisher: Cambridge University Press

Published: 2012-07-05

Total Pages: 574

ISBN-13: 1139510495

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The assessment and selection of oocytes and embryos is fundamental to the live birth rate data of all IVF units, the parameter that is used to gauge a clinic's success and credibility. This new atlas contains over 1000 high-quality images of oocytes, zygotes and embryos, presented with accompanying data on indications for treatment, stimulation type and duration, as well as short medical histories of each couple and final outcome of treatment. All images in the book can be downloaded from the accompanying CD-ROM. Structured on a patient-by-patient basis, the atlas describes 100 clinically documented case studies that follow the evolution of oocytes and zygotes between day two and day five. Pronuclear morphology and synchrony as well as embryo morphology are reported and described for each case. Written and produced by experienced embryologists, this practical atlas is an important resource for clinical embryologists and physicians in reproductive medicine.


In-Vitro Fertilization

In-Vitro Fertilization

Author: Kay Elder

Publisher: Cambridge University Press

Published: 2010-12-02

Total Pages: 285

ISBN-13: 9780521730723

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This fully updated new edition of a successful and popular practical guide is an indispensable account of modern in-vitro fertilization practice. Initial chapters cover theoretical aspects of gametogenesis and embryo development at the cellular and molecular level, while the latter half of the book describes the requisites for a successful IVF laboratory and the basic technologies in ART. Advanced techniques, including pre-implantation genetic diagnosis, vitrification and stem-cell technology, are comprehensively covered, providing up-to-date analyses of these groundbreaking technologies. This edition includes: • New practical techniques, including preservation of fertility for cancer patients, stem-cell biology/technology, vitrification and in-vitro maturation • A 'refresher' study review of fundamental principles of cell and molecular biology • The latest information available from animal and human research in reproductive biology Packed with a wealth of practical and scientific detail, this is a must for all IVF practitioners.


Practical Manual of In Vitro Fertilization

Practical Manual of In Vitro Fertilization

Author: Zsolt Peter Nagy

Publisher: Springer Science & Business Media

Published: 2012-04-23

Total Pages: 684

ISBN-13: 1441917802

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The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future. An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.


A Textbook of Clinical Embryology

A Textbook of Clinical Embryology

Author: Eliezer Girsh

Publisher: Cambridge University Press

Published: 2021-05-06

Total Pages: 257

ISBN-13: 110889464X

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Personnel working in assisted reproductive technology often lack the opportunities for dedicated training in the specialized techniques and technologies required for the procedures. As such, success in the form of live birth rates can range from over 50% to less than 10% per treatment cycle. This comprehensive introductory textbook is an essential resource for trainee embryologists, medical students and nurses. The recent revolutions in biotechnology and molecular biology involved in delivering assisted reproductive services are thoroughly discussed. Basic knowledge such as the development and physiology of both male and female reproductive systems is covered, with practical aspects of IVF including gamete and embryo manipulation, cryopreservation and genetic testing explained in detail. A full description of the optimal structure and management of the IVF laboratory is given, helping ensure procedures are safe and effective. Extensive and highly detailed colour illustrations bring the content to life and aids readers in their understanding.


Innate

Innate

Author: Kevin J. Mitchell

Publisher: Princeton University Press

Published: 2020-03-31

Total Pages: 305

ISBN-13: 0691204152

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"What makes you the way you are--and what makes each of us different from everyone else? In Innate, leading neuroscientist and popular science blogger Kevin Mitchell traces human diversity and individual differences to their deepest level: in the wiring of our brains. Deftly guiding us through important new research, including his own groundbreaking work, he explains how variations in the way our brains develop before birth strongly influence our psychology and behavior throughout our lives, shaping our personality, intelligence, sexuality, and even the way we perceive the world. We all share a genetic program for making a human brain, and the program for making a brain like yours is specifically encoded in your DNA. But, as Mitchell explains, the way that program plays out is affected by random processes of development that manifest uniquely in each person, even identical twins. The key insight of Innate is that the combination of these developmental and genetic variations creates innate differences in how our brains are wired--differences that impact all aspects of our psychology--and this insight promises to transform the way we see the interplay of nature and nurture. Innate also explores the genetic and neural underpinnings of disorders such as autism, schizophrenia, and epilepsy, and how our understanding of these conditions is being revolutionized. In addition, the book examines the social and ethical implications of these ideas and of new technologies that may soon offer the means to predict or manipulate human traits. Compelling and original, Innate will change the way you think about why and how we are who we are."--Provided by the publisher.