Aqueous Two-Phase Systems

Aqueous Two-Phase Systems

Author: Rajni Hatti-Kaul

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 442

ISBN-13: 1592590284

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A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.


CRC Handbook of Thermodynamic Data of Aqueous Polymer Solutions

CRC Handbook of Thermodynamic Data of Aqueous Polymer Solutions

Author: Christian Wohlfarth

Publisher: CRC Press

Published: 2004-01-06

Total Pages: 519

ISBN-13: 0203998200

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Providing the necessary basis for any developments of theoretical thermodynamic models, this book provides a complete collection of practical thermodynamic data for a variety of applications, including: basic and applied chemistry, chemical engineering, thermodynamic research, computational modeling, membrane science and technology, and environmental and green chemistry. The data -- which includes such developments as vapor-liquid and liquid-liquid equilibria, low-and high-pressure equilibrium data, enthalpic and volumetric data, and second virial coefficients -- is necessary when studying intermolecular interactions and gaining insights into the molecular nature of mixtures.


CRC Handbook of Thermodynamic Data of Polymer Solutions, Three Volume Set

CRC Handbook of Thermodynamic Data of Polymer Solutions, Three Volume Set

Author: Christian Wohlfarth

Publisher: CRC Press

Published: 2018-10-03

Total Pages: 1688

ISBN-13: 1482274906

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Providing valuable insight on physical behavior of polymer solutions, intermolecular interactions, and the molecular nature of mixtures, each volume in this one-of-a-kind handbook brings together reliable, easy-to-use entries, references, tables, examples, and appendices on experimental data from hundreds of primary journal articles, dissertations, and other published papers. This three-volume set presents hundreds of data sets including VLE/gas solubility isotherms, LLE and HPPE for polymer systems in supercritical fluids, as well as volumetric, enthalpic, and virial coefficient data sets, essential for handling industrial and laboratory processes involving all types of polymer systems.


Aqueous Biphasic Separations

Aqueous Biphasic Separations

Author: Robin D. Rogers

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 191

ISBN-13: 1461519535

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Proceedings of an American Chemical Society Symposium held in San Diego, California, March 13-14, 1994


CRC Handbook of Phase Equilibria and Thermodynamic Data of Aqueous Polymer Solutions

CRC Handbook of Phase Equilibria and Thermodynamic Data of Aqueous Polymer Solutions

Author: Christian Wohlfarth

Publisher: CRC Press

Published: 2012-08-10

Total Pages: 777

ISBN-13: 146655438X

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A large amount of experimental data has been published since the debut of the original CRC Handbook of Thermodynamic Data of Aqueous Polymer Solutions. Incorporating new and updated material, the CRC Handbook of Phase Equilibria and Thermodynamic Data of Aqueous Polymer Solutions provides a comprehensive collection of thermodynamic data of polymer solutions. It helps readers quickly retrieve necessary information from the literature, and assists researchers in planning new measurements where data are missing. A valuable resource for the modern chemistry field, the Handbook clearly details how measurements were conducted and methodically explains the nomenclature. It presents data essential for the production and use of polymers as well as for understanding the physical behavior and intermolecular interactions in polymer solutions.


The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering

The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering

Author: John Paul Frampton

Publisher: Frontiers Media SA

Published: 2020-01-13

Total Pages: 117

ISBN-13: 288963356X

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The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.


CRC Handbook of Phase Equilibria and Thermodynamic Data of Copolymer Solutions

CRC Handbook of Phase Equilibria and Thermodynamic Data of Copolymer Solutions

Author: Christian Wohlfarth

Publisher: CRC Press

Published: 2010-11-22

Total Pages: 552

ISBN-13: 1439850399

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Ten years after the debut of the expansive CRC Handbook of Thermodynamic Data of Copolymer Solutions, The CRC Handbook of Phase Equilibria and Thermodynamic Data of Copolymer Solutions updates and expands the world's first comprehensive source of this vital data. Author Christian Wohlfarth, a chemical thermodynamicist specializing in phase equilibr


Droplets of Life

Droplets of Life

Author: Vladimir N Uversky

Publisher: Academic Press

Published: 2022-11-09

Total Pages: 730

ISBN-13: 0128241756

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Droplets of Life: Membrane-Less Organelles, Biomolecular Condensates, and Biological Liquid–Liquid Phase Separation provides foundational information on the biophysics, biogenesis, structure, functions, and roles of membrane-less organelles. The study of liquid–liquid phase separation has attracted a lot of attention from disciplines such as cell biology, biophysics, biochemistry, and others trying to understand how, why, and what roles these condensates play in homeostasis and disease states in living organisms. This book's editor recruited a group of international experts to provide a current and authoritative overview of all aspects associated with this exciting area. Sections introduce membrane-less organelles (MLOs) and biomolecular condensates; MLOs in different sizes, shapes, and composition; and the formation of MLOs due to phase separation and how it can tune reactions, organize the intracellular environment, and provide a role in cellular fitness. . - Presents the first book to establish the foundations of this exciting research area - Combines biophysics, structural and cell biology, and biochemistry perspectives into a single volume - Edited and authored by world-leading scientists - Covers basic physical and biological principles and health and disease implications


Separations Using Aqueous Phase Systems

Separations Using Aqueous Phase Systems

Author: D. Fisher

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 476

ISBN-13: 1468456679

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The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.