The Surfaceome
The Surfaceome

Author: Kenneth R. Boheler

Publisher: Humana Press

Published: 2017-12-20

Total Pages: 348

ISBN-13: 9781493975518

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This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

Mass Spectrometry-Based Proteomics
Mass Spectrometry-Based Proteomics

Author: Kris Gevaert

Publisher: Springer Nature

Published: 2023-09-04

Total Pages: 337

ISBN-13: 1071634577

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This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Proteomics serves as an ideal guide to its subject for both novices in the field of proteomics as well as specialists.

Senolytics in Disease, Ageing and Longevity
Senolytics in Disease, Ageing and Longevity

Author: Daniel Muñoz-Espin

Publisher: Springer Nature

Published: 2020-04-27

Total Pages: 226

ISBN-13: 3030449033

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This book offers comprehensive information on the new and rapidly evolving science of identifying and targeting senescent cells, and on the exciting prospect of new diagnostic and therapeutic opportunities for stopping, and even reversing, the progression of disease and the deterioration of the human body due to ageing. According to recent United Nations data, by 2050 one in six people worldwide will be older than age 65, with peaks rising to one in four people in Europe and North America. Remarkably, the number of persons aged 80 years or older is expected to triple, from 143 million in 2019 to 426 million in 2050. First documented in the 1960s, the concept of cellular senescence as an underlying cause of ageing has been established in the course of the last decade. Using genetically engineered mouse models, researchers have demonstrated that the selective elimination of senescent cells can block and even reverse a number of age-related dysfunctions and pathologies, promoting both better health and longer life in the elderly. These include cardiovascular diseases; neurological disorders; type 1 and type 2 diabetes; inflammatory diseases; fibrosis; geriatric syndromes; chronic diseases resulting in organ dysfunction; the integrity of the musculoskeletal system; and cancer. Some senolytic agents have already progressed into trials. These include UBX0101 for the treatment of osteoarthritis (now in phase II), a cocktail of dasatinib and quercetin for the management of idiopathic pulmonary fibrosis and chronic kidney disease, and ABT-263 in combination with senescence-inducing chemotherapies for the treatment of advanced solid tumours. In addition, the book discusses pathways to early phase clinical trials and translational approaches in medicine and ageing, highlighting new opportunities as well as current limitations, challenges and alternatives. Given its scope, it will benefit a broad audience of advanced educators, researchers, graduate students and practitioners.

Proteomics Approaches to Unravel Virus - Vertebrate Host Interactions
Proteomics Approaches to Unravel Virus - Vertebrate Host Interactions

Author:

Publisher: Academic Press

Published: 2021-04-30

Total Pages: 270

ISBN-13: 0128230401

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Proteomics Approaches to Unravel Virus - Vertebrate Host Interactions, Volume 109 in the Advances in Virus Research series, highlights state-of-the art mass spectrometry techniques to elucidate the tight interplay of vertebrate viruses and their host cells. The volume includes chapters on Spatio-temporal resolution of host protein complexes during virus entry, Proteomic approaches to investigate gammaherpesvirus biology and associated tumorigenesis, Applications of Mass Spectrometry Imaging in Virus Research, Mapping surfaceome dynamics during viral infection, Characterization of proteolytic events in virus-host interactions, Dynamic protein network modulation upon viral infection, and much more. - Discusses the latest methodological breakthroughs in mass spectrometry-based proteomics - Reviews how technology has advanced our knowledge on virus-host interactions - Provides future perspectives on proteomics research in virology

Rheumatology E-Book
Rheumatology E-Book

Author: Marc C. Hochberg

Publisher: Elsevier Health Sciences

Published: 2018-01-08

Total Pages: 6453

ISBN-13: 0702073067

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Thoroughly revised and updated, the 7th Edition of Rheumatology remains a leading text in this fast-changing field, keeping you abreast of recent advances in medications, therapies, clinical trials, and much more. Dr. Mark Hochberg and his team of expert authors and editors cover everything you need to know -- from basic scientific principles to practical clinical management strategies, all in a user-friendly, accessible manner. Provides access to quick, concise videos depicting musculoskeletal ultrasound including anisotropy, "comet tail" needle artifact, rheumatoid arthritis synovitis, acute gout, and more. Uses a consistent, logical, reader-friendly format with templated content and large-scale images for efficient visual reference. Features 14 completely new chapters covering biomedical and translational science, disease and outcome assessment, including new imaging modalities and early emerging disease, clinical therapeutics, and patient management and rehabilitation. Includes updated content on the latest diagnostic perspectives and approaches to therapy including tapering down of treatment, pulmonary hypertension and scleroderma, psoriatic arthritis, and the latest oral anticoagulants. Covers hot topics such as pathogenesis and pathophysiology of axial spondyloarthritis, DXA and measurement of bone, infectious arthritis, and more. Shares the knowledge and expertise of internationally recognized rheumatoid arthritis expert and new editor, Dr. Ellen Gravallese. Expert ConsultTM eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, and references from the book on a variety of devices.

Food Safety
Food Safety

Author: Steven Ricke

Publisher: Academic Press

Published: 2015-07-08

Total Pages: 466

ISBN-13: 0128004045

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Food Safety: Emerging Issues, Technologies and Systems offers a systems approach to learning how to understand and address some of the major complex issues that have emerged in the food industry. The book is broad in coverage and provides a foundation for a practical understanding in food safety initiatives and safety rules, how to deal with whole-chain traceability issues, handling complex computer systems and data, foodborne pathogen detection, production and processing compliance issues, safety education, and more. Recent scientific industry developments are written by experts in the field and explained in a manner to improve awareness, education and communication of these issues. - Examines effective control measures and molecular techniques for understanding specific pathogens - Presents GFSI implementation concepts and issues to aid in implementation - Demonstrates how operation processes can achieve a specific level of microbial reduction in food - Offers tools for validating microbial data collected during processing to reduce or eliminate microorganisms in foods

Author:

Publisher: IOS Press

Published:

Total Pages: 6097

ISBN-13:

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Proteomics Approaches to Unravel Virus - Vertebrate Host Interactions
Proteomics Approaches to Unravel Virus - Vertebrate Host Interactions

Author: Gisa Gerold

Publisher: Elsevier

Published: 2021-05-05

Total Pages: 268

ISBN-13: 0128230428

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Proteomics Approaches to Unravel Virus - Vertebrate Host Interactions, Volume 109 in the Advances in Virus Research series, highlights state-of-the art mass spectrometry techniques to elucidate the tight interplay of vertebrate viruses and their host cells. The volume includes chapters on Spatio-temporal resolution of host protein complexes during virus entry, Proteomic approaches to investigate gammaherpesvirus biology and associated tumorigenesis, Applications of Mass Spectrometry Imaging in Virus Research, Mapping surfaceome dynamics during viral infection, Characterization of proteolytic events in virus-host interactions, Dynamic protein network modulation upon viral infection, and much more. Discusses the latest methodological breakthroughs in mass spectrometry-based proteomics Reviews how technology has advanced our knowledge on virus-host interactions Provides future perspectives on proteomics research in virology

P5 Medicine and Justice
P5 Medicine and Justice

Author: Santo Davide Ferrara

Publisher: Springer

Published: 2018-03-27

Total Pages: 643

ISBN-13: 3319670921

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This book describes the state of the art and future prospects of the most important bio-medicolegal subdisciplines in the post-genomic framework of personalized medicine. Focusing on the three main themes Innovation, Unitariness and Evidence, the book addresses a wide range of topics, including: Bio-Medicolegal and Criminological Sciences, Forensic Pathology and Anthropology, Clinical and Forensic Medicine in Living Persons (from Interpersonal Violence to Personal Injury and Damage, Malpractice, Personal Identification and Age Estimation), Forensic Genetics and Genomics, and Toxicology and Imaging. The unitariness of the “Bio-Medicolegal Sciences”, historically founded on the accuracy and rigor of the methods of ascertainment and criteria of evaluation, should be re-established on the basis of molecular evidence, and used to promote Personalized Justice. Taken together, the book’s conclusions and future perspectives outline a vision of transdisciplinary innovation and future evidence in the framework of personalized justice.

Advances in Animal Disease Diagnosis
Advances in Animal Disease Diagnosis

Author: Suresh Kumar Gahlawat

Publisher: CRC Press

Published: 2021-06-15

Total Pages: 423

ISBN-13: 1000397378

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Advances in Animal Disease Diagnosis: Infectious animal diseases caused by pathogenic microorganisms such as bacteria, fungi, and viruses threaten the health and well-being of wildlife, livestock and human populations, limit productivity and significantly increase economic losses to each sector. Pathogen de-tection is an important step for the diagnosis and successful treatment of animal diseases as well as control management in farm and field conditions. The conventional techniques employed to diagnose pathogens in livestock species are time-consuming and sometimes give inconclusive results. On the contrary, molecular techniques have the potential to diag-nose known pathogens/conditions quickly, reliably, and unequivocally as well as for novel pathogen detection. New advances in diagnostics and vaccine design using genomics have developed powerful new methods that have also set the stage for the enhanced diagnosis, surveillance, and control of infectious diseases. High-throughput sequencing (HTS), for ex-ample, uses the latest DNA sequencing platforms in the detection, identification, and detailed analysis of both pathogen and host genomes. This book will explore some key opportunities in the context of animal health, such as the detection of new microorganisms and the development of improved diagnosis of emerging or re-emerging diseases and other clinical conditions, viz. biosensors, nanotools, and omics technologies. Features • Details comprehensive knowledge on the latest molecular techniques for animal disease diagnosis and management • Examines how DNA-based diagnostic techniques will assist international efforts to control the introduction of exotic diseases into new geographic areas • Describes the latest molecular assays for the rapid and accurate detection of pathogens • Helps in working towards meeting the global challenge for sustainable food production and the eradication of poverty • With new biotechnological developments, this fully updated book is a treasure trove of the latest information in animal and medical science