Seafaring Scientist

Seafaring Scientist

Author: Lester D. Stephens

Publisher: Univ of South Carolina Press

Published: 2006

Total Pages: 264

ISBN-13: 9781570036422

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Infused with a sense of adventure and zeal for discovery, Seafaring Scientist recounts the achievements of a giant in the field of marine biology. Alfred Goldsborough Mayor (18681922), a Harvard-trained marine biologist and close associate of Alexander Agassiz, founded and directed on behalf of the Carnegie Institution the first tropical marine biological laboratory in the Western hemisphere. Located on Loggerhead Key in the Gulf of Mexico, the Tortugas Laboratory attracted some of America's most brilliant scientists. Mayor himself achieved international prominence in the field of biology for his authoritative work on jellyfishes and coral reefs.


Green Fluorescent Protein

Green Fluorescent Protein

Author: Martin Chalfie

Publisher: John Wiley & Sons

Published: 2005-11-18

Total Pages: 486

ISBN-13: 0471739480

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Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.