Techniques in Molecular Medicine
Techniques in Molecular Medicine

Author: Friedhelm Hildebrandt

Publisher: Springer Science & Business Media

Published: 2013-11-11

Total Pages: 394

ISBN-13: 3642598110

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This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual

Methods in Molecular Medicine
Methods in Molecular Medicine

Author: Yusuf Tutar

Publisher: BoD – Books on Demand

Published: 2021-01-20

Total Pages: 166

ISBN-13: 1839627263

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Treatment risk and response to therapy prediction can be forecasted through early diagnosis, which improves prognosis reliability and effectiveness of therapies. This book covers contemporary advances in molecular markers, disease-causing variants, retroelements, and the basis of distinct diseases.

Molecular Biology Techniques
Molecular Biology Techniques

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Modern Clinical Molecular Techniques
Modern Clinical Molecular Techniques

Author: Peter Hu

Publisher: Springer Science & Business Media

Published: 2012-05-11

Total Pages: 428

ISBN-13: 1461421705

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This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice. Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.

Molecular Biology Techniques
Molecular Biology Techniques

Author: Walt Ream

Publisher: Academic Press

Published: 1998-11-17

Total Pages: 245

ISBN-13: 0080536824

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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. - Provides well-tested experimental protocols for each technique - Lists the reagents and preparation of each experiment separately - Contains a complete schedule of experiments and the preparation required - Includes study questions at the end of each chapter

Molecular Diagnostics
Molecular Diagnostics

Author: Wayne W. Grody

Publisher: Academic Press

Published: 2009-11-06

Total Pages: 518

ISBN-13: 0080919049

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Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. Molecular Diagnostics provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting. - Serves as the definitivereference for molecular pathologists worldwide - Covers a variety of molecular techniques including next generation sequencing, tumor somatic cell genotyping, infectious and genetic disease tecting, and pharmacogenetics - Discusses in the detail issues concerning quality assurance, regulation, ethics, and future directions for the science

Oral Biology
Oral Biology

Author: Gregory J. Seymour

Publisher: Springer Nature

Published: 2022-11-23

Total Pages: 523

ISBN-13: 1071627805

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This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

Author: Andreas Hofmann

Publisher: Cambridge University Press

Published: 2018-04-19

Total Pages: 962

ISBN-13: 1108365256

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Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.

Molecular Medicine for Clinicians
Molecular Medicine for Clinicians

Author: Barry Mendelow

Publisher: Wits University Press

Published: 2008-01-01

Total Pages: 526

ISBN-13: 1868144658

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The insights following the wake of the Human Genome project are radically influencing our understanding of the molecular basis of life, health and disease. The improved accuracy and precision of clinical diagnostics is also beginning to have an impact on therapeutics in a fundamental way. This book is suitable for undergraduate medical students, as part of their basic sciences training, but is also relevant to interested under- and postgraduate science and engineering students. It serves as an introductory text for medical registrars in virtually all specialties, and is also of value to the General Practitioner wishing to keep up to date, especially in view of the growing, internet-assisted public knowledge of the field. There is a special focus on the application of molecular medicine in Africa and in developing countries elsewhere.