Subnuclear Components
Subnuclear Components

Author: G. D. Birnie

Publisher: Elsevier

Published: 2016-01-21

Total Pages: 349

ISBN-13: 1483103404

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Subnuclear Components: Preparation and Fractionation focuses on the isolation of subnuclear components of eukaryotic cells. The book first discusses the isolation of nuclear envelopes from whole tissues. Topics include nuclear envelope in situ; general consideration and evaluation of isolation methods; and nuclear envelope isolation methods. The text describes the isolation of nucleoli, including the isolation of nucleoli from oocytes, nuclei, and physarum, and isolation of plant nucleoli. The book focuses on the preparation, characterization, and fractionation of chromatin. Emphasis is on the preparation of chromatin from interphase and metaphase cells; contaminants of chromatin; characteristics of isolated chromatin; and dissociation and reassociation of chromatin. The text also discusses fractionation and isolation of histones, including the preparation of individual histone fractions and isolation and purification of the five main histone fractions of calf thymus during one preparation. The book also looks at the preparation and properties of chromatin non-histone proteins, including isolation of nuclear RNA and isolation of DNA from eukaryotic cells. The selection is a good source of data for readers interested in the isolation of subnuclear constituents of eukaryotic cells.

The Nucleus
The Nucleus

Author: Ronald Hancock

Publisher: Humana Press

Published: 2014-10-14

Total Pages: 0

ISBN-13: 9781493916795

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This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.

Current Catalog
Current Catalog

Author: National Library of Medicine (U.S.)

Publisher:

Published: 1979

Total Pages:

ISBN-13:

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First multi-year cumulation covers six years: 1965-70.

Spectral Techniques In Proteomics
Spectral Techniques In Proteomics

Author: Daniel S. Sem

Publisher: CRC Press

Published: 2007-03-30

Total Pages: 464

ISBN-13: 1420017098

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Facilitating the innovation, development, and application of new spectroscopic methods in proteomics, Spectral Techniques in Proteomics provides a broad overview of the spectroscopic toolbox that can be used, either with proteome or sub-proteome mixtures or with individual/purified proteins studied in parallel. It gives a modest overview of

Plant Biochemistry
Plant Biochemistry

Author: James Bonner

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 944

ISBN-13: 032313954X

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Plant Biochemistry, Third Edition examines the fundamental aspects of plant biochemistry and biology, including taxonomy, morphology, ecology, horticulture, agronomy, and phytopathology. It discusses the substructures and subfunctions of plant cells, the basic metabolism of plants, and the mechanism and regulation as well as physiological significance of various pathways of photosynthetic carbon dioxide assimilation. Comprised of 26 chapters, this edition begins with an overview of the subcellular components of the plant cell, the overall logic or strategy that the cell uses, and the operation of individual subcellular systems. It discusses the plant ribosomes and nuclei, biosynthesis and assembly of cell membranes in plants, distribution and functional roles of microbodies in plants, photosynthesis and the general biology of chloroplasts, and plant microtubules. The remaining chapters focus on the biochemistry and functions of vacuoles, the primary cell wall and its biogenesis, the regulation of enzyme activity in metabolic pathways, the monosaccharides and oligosaccharides, and the lipid metabolism. The book concludes with a chapter on biological nitrogen fixation and its practical applications in agriculture. This book is a valuable resource for biochemists and plant biologists as well as advanced students or professional workers in plant sciences.

The Nucleus
The Nucleus

Author: Ronald Hancock

Publisher: Humana Press

Published: 2008-08-28

Total Pages: 360

ISBN-13: 9781588299772

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Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities.

Nuclear Structures
Nuclear Structures

Author: A. J. MacGillivray

Publisher: Elsevier

Published: 2013-10-22

Total Pages: 223

ISBN-13: 1483103382

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Nuclear Structures: Isolation and Characterization focuses on techniques for the isolation of molecular and macromolecular components of cell nuclei. The book first discusses nuclear matrices, including isolation of nuclear matrices and notes on the method. The text examines nucleoids. Nucleoid preparation and morphology, characterization of nucleoids, experimental uses of nucleoids, and criticism of nucleoid preparation are underscored. The book also discusses nuclear envelopes, polynucleosomes and monomer nucleosomes, and metaphase chromosomes. The text also examines DNA tight-binding proteins, including the definition and preparation of DNA tight-binding proteins; immunological characterization of tight-binding proteins; and DNA binding characteristics. The book also examines nucleoli and preribosomal ribonucleoprotein particles. Methods for the isolation of nucleoli; assessment of nucleolar preparations; and isolation of preribosomal ribonucleoprotein particles are discussed. The text also covers ribonucleoprotein particles containing heterogeneous nuclear RNA and nuclear RNP particles containing small RNAs. The selection is a good reference for readers wanting to study the isolation of molecular and macromolecular components of cell nuclei.

Comprehensive Biophysics
Comprehensive Biophysics

Author:

Publisher: Academic Press

Published: 2012-04-12

Total Pages: 3533

ISBN-13: 0080957188

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Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource