Subcellular Components
Subcellular Components

Author: G. D. Birnie

Publisher: Elsevier

Published: 2014-05-20

Total Pages: 333

ISBN-13: 1483162842

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Subcellular Components: Preparation and Fractionation talks about cells and particles' components, including their preparation and fractionation. The book includes theories and answers to questions that are relevant to the study. The first chapter of the book details various facts about homogenization of mammalian cells. This chapter presents the results of studies on solid tissues and single-cell suspensions; the author then offers his conclusion of the study. The next two chapters highlight the methods on isolating nuclei, including the guides for standard assessment and the procedure of isolation, along with analysis of nuclei biochemical properties. The main topics in Chapter 4 are mitochondria from animal tissues and yeasts; this chapter also discusses the preparation for a rat-liver, blowfly flight-muscle, yeast, and brain mitochondria. The chapter that follows widely talks about lysosomes, including its historical background, centrifugal method, and related topics. In the next several chapters, the topics covered include purification, isolation, preparation, and separation of cells including plasma-membrane, polysomes, ribosomes, microsomes, and microvilli. The book serves as a great reference for undergraduates and postgraduates in the field, as it contains a thorough discussion of various relevant studies.

Subcellular Fractionation
Subcellular Fractionation

Author: Paul R. Pryor

Publisher:

Published: 2015

Total Pages: 0

ISBN-13: 9781621820420

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Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.

Subcellular Fractionation
Subcellular Fractionation

Author: J. M. Graham

Publisher: IRL Press

Published: 1997-01-30

Total Pages: 362

ISBN-13: 0191591610

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Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.

Subnuclear Components
Subnuclear Components

Author: G. D. Birnie

Publisher: Elsevier

Published: 2016-01-21

Total Pages: 349

ISBN-13: 1483103404

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Subnuclear Components: Preparation and Fractionation focuses on the isolation of subnuclear components of eukaryotic cells. The book first discusses the isolation of nuclear envelopes from whole tissues. Topics include nuclear envelope in situ; general consideration and evaluation of isolation methods; and nuclear envelope isolation methods. The text describes the isolation of nucleoli, including the isolation of nucleoli from oocytes, nuclei, and physarum, and isolation of plant nucleoli. The book focuses on the preparation, characterization, and fractionation of chromatin. Emphasis is on the preparation of chromatin from interphase and metaphase cells; contaminants of chromatin; characteristics of isolated chromatin; and dissociation and reassociation of chromatin. The text also discusses fractionation and isolation of histones, including the preparation of individual histone fractions and isolation and purification of the five main histone fractions of calf thymus during one preparation. The book also looks at the preparation and properties of chromatin non-histone proteins, including isolation of nuclear RNA and isolation of DNA from eukaryotic cells. The selection is a good source of data for readers interested in the isolation of subnuclear constituents of eukaryotic cells.