Soil enzymes are one of the vital key mediators involved in nutrient recycling and the decomposition of organic matter and thereby in maintaining soil quality and fertility. This Soil Biology volume covers the various facets of soil enzymes, such as their functions, biochemical and microbiological properties and the factors affecting their activities. Enzymes in the rhizosphere, in forest soils, and in volcanic ash-derived soils are described. Soil enzymes covered include phosphohydrolases, lignocellulose-degrading enzymes, phenol oxidases, fungal oxidoreductases, keratinases, pectinases, xylanases, lipases and pectinases. Several chapters treat the soil enzymatic activities in the bioremediation of soils contaminated with pesticides and pollutants such as oil, chlorinated compounds, synthetic dyes and aromatic hydrocarbons. The role of soil enzymes as bioindicators is a further important topic addressed.
Soil sampling for microbiological analysis; Statistical treatment of microbial data; Soil sterilization; Soil water potencial; Most probable number counts; Light microscopic methods for studying soil microorganisms; Viruses; Recovery and enumeration of viable bacteria; Coliform bacteria; Autotrophic nitrifying bacteria; Free-living dinitrogen-fixing bacteria; Legume nodule symbionts; Anaerobic bacteria and processes; Denitrifiers; Actiomycetes; Frankia and the actinorhizal symbiosis; Filamentous fungi; Vesicular-arbuscular mycorrhizal fungi; Isolation of microorganisms producting antibiotics; Microbiological procedures for biodegradation research; Algae and cyanobacteria; Marking soil bacteria with lacZY; Detection of specific DNA sequences in environmental sample via polymerase chaim reaction; Isolation and purification of bacterial DNA from soil; Microbial biomass; Soil enzymes; Carbon mineralization; Isotopic methods for the study of soil organic matter dynamics ; Practical considerations in the use of nitrogen tracers in agricultural and environmental research; Nitrogen availability; Nitrogen mineralization, immobilization, and nitrification; Dinitrogen fixation; Measuring denitrification in the field; Sulfur oxidation and reduction in soils; Iron and manganese oxidation and reduction.
Methods of Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Readers will especially benefit from the step-by-step explanation of the lab procedures, as well as background information for using these methods effectively and analyzing data. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background informaton, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. Written for the researcher, but welcoming to those new to soil enzymology, the introduction includes conceptual information to orient those who are not familiar with these methods but want to use them. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.
The need to understand the biological processes that are important for essential aquatic and terrestrial ecosystem function has prompted much research into the field of ecological enzymology. This book presents the two broad areas of application in a compilation of reviews by 21 international experts in their respective fields. The first explores enzymatic activities to assess the processes or mechanisms that operate in a given system, such as the rhizosphere, plant leaves and shoots, soil surfaces, and biofilms. The second considers enzymes or microbial cells as sensors to detect microbial activity and stresses due to pollution, management, or climatic change in both aquatic and terrestrial ecosystems.
Microbial enzymes play a vital role in maintaining soil health and removing pollutants from contaminated land. Soil microflora is closely associated with maintaining soil fertility, and the use of chemical pesticides, fertilizers and other volatile sprays in agriculture threatens the health ofthe microbial population in the soil. Every single particle of healthy soil contains millions of bacteria, which interact with the nutrients available, sustaining the nutrient cycle and making this microflora an essential component of life on earth. How do microbes help in the nutrient cycle? Either by intracellular digestion of macromolecules and converting these into smaller units in their metabolic pathways, or by secreting enzymes into the extracellular environment to facilitate the conversion of complex macromolecules into micro-molecules that can be easily absorbed by other living species. To meet demands for energy and food for the growing global population, it is important to protect agricultural land from contamination and maintain its productivity. Heavy metal ions from contaminated land canenter crops, fish or aquatic organismsvia contaminated water, and theseare then taken up by the human body, where they can accumulate and alter the normal microflora. The microbiological component of the soil is ahighly complex system and is still not fully understood. How do microbes survive in the changing physicochemical environment of soil?. This book helps readers understand the mechanism, various routes of microbialsoil remediation, the interactionsof different genera, and how microbial enzymes support the sustainable restoration of healthy soil.
This book addresses issues arising from discharge of effluents from sugar industry on to surrounding land or into a water body such as physicochemical properties of soil, changes in the micro flora, quantification of soil enzyme activities as influenced by effluents. Disposal of effluents without neutralization has become general practice. These effluents are chemically heterogeneous, contain organic and inorganic pollutants including, sugar baggage, molasses, carbonates, bicarbonates. The impact of sugar industry effluents on microbial activities in terrestrial ecosystem is scanty. There is also significant interest in the study of soil enzymes because such effect reflects the potential capacity of a soil to perform certain biological transformation of soil fertility.
Provides up-to-date reviews on the conditions that affect the quality of soil and on the methods to measure the effects of soil management and bioremediation--focusing on indigenous or introduced microorganisms with the capacity to remediate pollutants.
Learn from this integrated approach to the management and restoration of ecosystems edited by an international leader in the field The Handbook of Ecological and Ecosystem Engineering delivers a comprehensive overview of the latest research and practical developments in the rapidly evolving fields of ecological and ecosystem engineering. Beginning with an introduction to the theory and practice of ecological engineering and ecosystem services, the book addresses a wide variety of issues central to the restoration and remediation of ecological environments. The book contains fulsome analyses of the restoration, rehabilitation, conservation, sustainability, reconstruction, remediation, and reclamation of ecosystems using ecological engineering techniques. Case studies are used to highlight practical applications of the theory discussed within. The material in the Handbook of Ecological and Ecosystem Engineering is particularly relevant at a time when the human population is dramatically rising, and the exploitation of natural resources is putting increasing pressure on planetary ecosystems. The book demonstrates how modern scientific ecology can contribute to the greening of the environment through the inclusion of concrete examples of successful applied management. The book also includes: A thorough discussion of ecological engineering and ecosystem services theory and practice An exploration of ecological and ecosystem engineering economic and environmental revitalization An examination of the role of soil meso and macrofauna indicators for restoration assessment success in a rehabilitated mine site A treatment of the mitigation of urban environmental issues by applying ecological and ecosystem engineering A discussion of soil fertility restoration theory and practice Perfect for academic researchers, industry scientists, and environmental engineers working in the fields of ecological engineering, environmental science, and biotechnology, the Handbook of Ecological and Ecosystem Engineering also belongs on the bookshelves of environmental regulators and consultants, policy makers, and employees of non-governmental organizations working on sustainable development.
The disturbance of soils, like other phenomena of environmental pollution, encountered in so many areas all over the world, has become a subject of extensive concern and has led to a vast amount of literature in the field of enzymology, too.The book is the first comprehensive and updated review of the enzymological literature on three great categories of disturbed soils, including their remediation. The volume consists of three sections: I. Enzymology of oil-contaminated soils; II. Enzymology of soils affected by industrial emissions; and III, enzymology of technogenic soils. Within each section the reviewed studies are grouped by country.The volume will be of great value to soil scientists, environmental scientists, conservationists, and many other scientists in these and related fields.
