Small Angle Scattering Part A: Methods for Structural Investigation
Small Angle Scattering Part A: Methods for Structural Investigation

Author:

Publisher: Academic Press

Published: 2022-11-19

Total Pages: 578

ISBN-13: 0323991807

DOWNLOAD EBOOK

Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on Small Angle Scattering: Methods for Structural Investigation

Structure Analysis by Small-Angle X-Ray and Neutron Scattering
Structure Analysis by Small-Angle X-Ray and Neutron Scattering

Author: L.A. Feigin

Publisher: Springer Science & Business Media

Published: 2013-11-11

Total Pages: 339

ISBN-13: 1475766246

DOWNLOAD EBOOK

Small-angle scattering of X rays and neutrons is a widely used diffraction method for studying the structure of matter. This method of elastic scattering is used in various branches of science and technology, includ ing condensed matter physics, molecular biology and biophysics, polymer science, and metallurgy. Many small-angle scattering studies are of value for pure science and practical applications. It is well known that the most general and informative method for investigating the spatial structure of matter is based on wave-diffraction phenomena. In diffraction experiments a primary beam of radiation influences a studied object, and the scattering pattern is analyzed. In principle, this analysis allows one to obtain information on the structure of a substance with a spatial resolution determined by the wavelength of the radiation. Diffraction methods are used for studying matter on all scales, from elementary particles to macro-objects. The use of X rays, neutrons, and electron beams, with wavelengths of about 1 A, permits the study of the condensed state of matter, solids and liquids, down to atomic resolution. Determination of the atomic structure of crystals, i.e., the arrangement of atoms in a unit cell, is an important example of this line of investigation.

Biological Small Angle Scattering: Techniques, Strategies and Tips
Biological Small Angle Scattering: Techniques, Strategies and Tips

Author: Barnali Chaudhuri

Publisher: Springer

Published: 2017-12-07

Total Pages: 269

ISBN-13: 981106038X

DOWNLOAD EBOOK

This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.

Small Angle Scattering Part A: Methods for Structural Investigation
Small Angle Scattering Part A: Methods for Structural Investigation

Author:

Publisher: Elsevier

Published: 2022-10-15

Total Pages: 576

ISBN-13: 0323991793

DOWNLOAD EBOOK

Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Small Angle Scattering: Methods for Structural Investigation

Small-Angle Scattering (Neutrons, X-Rays, Light) from Complex Systems
Small-Angle Scattering (Neutrons, X-Rays, Light) from Complex Systems

Author: Eugen Mircea Anitas

Publisher: Springer

Published: 2019-08-08

Total Pages: 121

ISBN-13: 3030266125

DOWNLOAD EBOOK

This book addresses the basic physical phenomenon of small-angle scattering (SAS) of neutrons, x-rays or light from complex hierarchical nano- and micro-structures. The emphasis is on developing theoretical models for the material structure containing self-similar or fractal clusters. Within the suggested framework, key approaches for extracting structural information from experimental scattering data are investigated and presented in detail. The range of parameters which can be obtained pave the road towards a better understanding of the correlations between geometrical and various physical properties (electrical, magnetic, mechanical, optical, dynamical, transport etc.) in fractal nano- and micro-materials.

Soft-Matter Characterization
Soft-Matter Characterization

Author: Redouane Borsali

Publisher: Springer Science & Business Media

Published: 2008-07-28

Total Pages: 1490

ISBN-13: 140204464X

DOWNLOAD EBOOK

This 2-volume set includes extensive discussions of scattering techniques (light, neutron and X-ray) and related fluctuation and grating techniques that are at the forefront of this field. Most of the scattering techniques are Fourier space techniques. Recent advances have seen the development of powerful direct imaging methods such as atomic force microscopy and scanning probe microscopy. In addition, techniques that can be used to manipulate soft matter on the nanometer scale are also in rapid development. These include the scanning probe microscopy technique mentioned above as well as optical and magnetic tweezers.

Neutron Scattering in Biology
Neutron Scattering in Biology

Author: Jörg Fitter

Publisher: Springer Science & Business Media

Published: 2006-05-01

Total Pages: 571

ISBN-13: 3540291113

DOWNLOAD EBOOK

The advent of new neutron facilities and the improvement of existing sources and instruments world wide supply the biological community with many new opportunities in the areas of structural biology and biological physics. The present volume offers a clear description of the various neutron-scattering techniques currently being used to answer biologically relevant questions. Their utility is illustrated through examples by some of the leading researchers in the field of neutron scattering. This volume will be a reference for researchers and a step-by-step guide for young scientists entering the field and the advanced graduate student.

Scattering Methods in Structural Biology Part B
Scattering Methods in Structural Biology Part B

Author:

Publisher: Elsevier

Published: 2023-01-12

Total Pages: 462

ISBN-13: 0323991823

DOWNLOAD EBOOK

Scattering Methods in Structural Biology, Part B, Volume 676 in the Methods in Enzymology serial, highlights advances in the field, presenting chapters on Quality controls, Refining biomolecular structures and ensembles by SAXS-driven molecular dynamics simulations, Data analysis and modelling of small-angle scattering data with contrast variation, Observing protein degradation in solution by the PAN-20S proteasome complex: state-of-the-art and future perspectives of TR-SANS as a complementary tool to NMR, crystallography and Cryo-EM, Extracting structural insights from chemically-specific soft X-ray scattering, Reconstruction of 3D density of biological macromolecules from solution scattering, ATSAS- present state and new developments in computational methods, and much more. Additional chapters cover Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles (FoXSDock and MultiFoXS), Validation of macromolecular flexibility in solution by SAXS, Combining NMR, SAXS and SANS to characterize the structure and dynamics of protein complexes, Application of Molecular Simulation Methods to Analyze SAS Data, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology serial - Updated release includes the latest information on Small Angle Scattering Methods for Structural Interpretation

Scattering Techniques Applied to Supramolecular and Nonequilibrium Systems
Scattering Techniques Applied to Supramolecular and Nonequilibrium Systems

Author: Sow Hsin Chen

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 909

ISBN-13: 1468440616

DOWNLOAD EBOOK

This Advanced Study Institute was held at \-lellesley College, Wellesley, MA. , from 3 to 12 August 1980. It followed by four years the second "Capri ~,chool on Photon Correlation Spectroscopy". During the intervening period there had been many new applications of dynamic light scattering techniques to the study of systems whose properties depend either on collective molecular interactions or on the formation or activity of supramo1ecu1ar structures. Con sequently, emphasis at this conference was on light scattering studies of subjects such as dynamical correlations in dense polymer solutions, phase transitions in gels, spinodal decomposition of binary fluids, Benard instabilities in nonequilibrium fluids, the formation of micelles and phospholipid vesicles, and movements of the molecular assemblies of muscle tissue. The instructional pro gramme also included tutorial lectures on two complementary spec troscopic techniques which have benefited from dramatic advances in instrumentation, these being small angle X-ray (SAXS) and small angle neutron (SANS) scattering. Strong cold neutron and synchro tron X-ray sources have become available, and data now can be acquired rapidly with newly developed position-sensitive detectors. Several reviews of recent applications of SAXS and SANS were also provided. The organizers of the ASI hoped to provide a forum for theoreticians and experimentalists to assess advances in fields which, although related, were sufficiently different that a great deal of unfamiliar information could be communicated. The order ing of the papers in this volume closely approximates that of the talks presented at the Advanced Study Institute.