Single Molecule Investigations Into DNA Replication, Recombination and Repair
Author:
Publisher:
Published: 2014
Total Pages:
ISBN-13:
DOWNLOAD EBOOKPosts
The DNA Replication-Repair Interface
Author:
Publisher: Academic Press
Published: 2021-11-12
Total Pages: 454
ISBN-13: 0323907342
DOWNLOAD EBOOKReplication-Coupled Repair, Volume 661 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including the Repair of replication-born DNA breaks by sister chromatid recombination, High resolution and high throughput DNA cyclization measurements to interrogate DNA bendability, A programmable detection method for genomic signatures: from disease diagnosis to genome editing, Characterization of the telomerase modulating activities of yeast DNA helicases, Eukaryotic DNA replication with purified budding yeast proteins, Single molecule studies of yeast Rad51 paralogs, Light activation and deactivation of Cas9 for DNA repair studies, and more. Other chapters explore MIDAS: Direct sequencing to map mitotic DNA synthesis and common fragile sites at high precision, Studying the DNA damage response in embryonic systems, GLASS-ChIP to map Mre11 cleavage sites in the human genome, New chemical biology approaches to trap reaction intermediates in living cells, Single-molecule imaging approaches for monitoring replication fork conflicts at genomic DNA G4 structures and R-loops in human cells, Monitoring the replication of structured DNA through heritable epigenetic change, Visualizing replication fork encounters with DNA interstrand crosslinks, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Includes the latest information on replication-coupled repair
DNA Replication, Recombination, and Repair
Author: Fumio Hanaoka
Publisher: Springer
Published: 2016-01-22
Total Pages: 548
ISBN-13: 443155873X
DOWNLOAD EBOOKThis book is a comprehensive review of the detailed molecular mechanisms of and functional crosstalk among the replication, recombination, and repair of DNA (collectively called the "3Rs") and the related processes, with special consciousness of their biological and clinical consequences. The 3Rs are fundamental molecular mechanisms for organisms to maintain and sometimes intentionally alter genetic information. DNA replication, recombination, and repair, individually, have been important subjects of molecular biology since its emergence, but we have recently become aware that the 3Rs are actually much more intimately related to one another than we used to realize. Furthermore, the 3R research fields have been growing even more interdisciplinary, with better understanding of molecular mechanisms underlying other important processes, such as chromosome structures and functions, cell cycle and checkpoints, transcriptional and epigenetic regulation, and so on. This book comprises 7 parts and 21 chapters: Part 1 (Chapters 1–3), DNA Replication; Part 2 (Chapters 4–6), DNA Recombination; Part 3 (Chapters 7–9), DNA Repair; Part 4 (Chapters 10–13), Genome Instability and Mutagenesis; Part 5 (Chapters 14–15), Chromosome Dynamics and Functions; Part 6 (Chapters 16–18), Cell Cycle and Checkpoints; Part 7 (Chapters 19–21), Interplay with Transcription and Epigenetic Regulation. This volume should attract the great interest of graduate students, postdoctoral fellows, and senior scientists in broad research fields of basic molecular biology, not only the core 3Rs, but also the various related fields (chromosome, cell cycle, transcription, epigenetics, and similar areas). Additionally, researchers in neurological sciences, developmental biology, immunology, evolutionary biology, and many other fields will find this book valuable.
Grab, Manipulate and Watch Single DNA Molecule Replication
Author:
Publisher:
Published: 2023
Total Pages: 0
ISBN-13: 9789464199550
DOWNLOAD EBOOKDNA replication is a critical process intrinsic to the sustenance and propagation of life, involving a symphony of enzymes including helicases, polymerases, and single-stranded DNA binding proteins (SSBs). These enzymes collaboratively ensure the precise duplication of genetic information, a process that, despite significant research, has elements yet to be fully elucidated, especially intermediary steps and the dynamic engagements between replicative proteins. Single-molecule techniques have recently blossomed, offering enhanced insights into the real-time dynamics and interactions of individual molecules in their natural settings, unveiling obscured intermediate steps and enzyme conformational changes. Chapter 1 outlines the thesis's primary aim, elucidating DNA replication mechanisms. Starting with fundamental biological notions, this chapter transitions to discuss the non-equilibrium nature of living systems, emphasizing the role of single-molecule investigations. Such studies have enhanced our understanding of non-equilibrium systems, revealing cellular mechanisms and influencing factors. SSBs are crucial for maintaining genome integrity as they bind to ssDNA and coordinate with various proteins involved in DNA replication, recombination, and repair. Chapter 2 offers a comprehensive overview of recent advances in our understanding of SSBs, as elucidated by single-molecule assays such as optical tweezers, magnetic tweezers, Förster resonance energy transfer, and their combinations. These techniques have provided novel insights into the dynamics of SSB binding to ssDNA and its interactions with other proteins, emphasizing the central role of SSB in modulating the activities of other proteins. Chapter 3 presents the single-molecule observations of the T7 bacteriophage single-stranded DNA-binding protein (gp2.5) binding to ssDNA. Our experiments demonstrate the significant influence of the base sequence, ssDNA conformation, and the acidic terminal domain of T7 gp2.
Molecular Themes in DNA Replication
Author: Lynne Suzanne Cox
Publisher: Royal Society of Chemistry
Published: 2009
Total Pages: 467
ISBN-13: 0854041648
DOWNLOAD EBOOKWritten by leading experts, this learned but accessible book highlights the latest work on eukaryotic DNA replication.
Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods
Author:
Publisher: Academic Press
Published: 2016-10-28
Total Pages: 618
ISBN-13: 0128095474
DOWNLOAD EBOOKSingle-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion
Single Molecule Analysis
Author: Erwin J. G. Peterman
Publisher: Humana Press
Published: 2011-09-15
Total Pages: 317
ISBN-13: 9781617792816
DOWNLOAD EBOOKLife scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.
Molecular Biology of the Cell
Author:
Publisher:
Published: 2002
Total Pages: 0
ISBN-13: 9780815332183
DOWNLOAD EBOOKSingle-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods
Author:
Publisher: Academic Press
Published: 2017-01-03
Total Pages: 486
ISBN-13: 0128095032
DOWNLOAD EBOOKSingle-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion
mechanistic studies of DNA replication and genetic recombination
Author: Bruce Alberts
Publisher: Elsevier
Published: 2012-12-02
Total Pages: 1034
ISBN-13: 032314179X
DOWNLOAD EBOOKMechanistic Studies of DNA Replication and Genetic Recombination emerged from a symposium on DNA replication and genetic recombination held from March 16-21, 1980 in Keystone, Colorado. The event featured 30 plenary session talks, 13 workshop discussion groups, and the 210 poster sessions. The studies described in this book are paving the way for the elucidation of other basic genetic mechanisms, including ""new"" areas in molecular genetics such as those of eukaryotic gene expression and the transposition of mobile genetic elements. This book is divided into 10 parts: summaries of workshop discussion groups (Part I); studies on eukaryotic model systems for DNA replication (Part II); studies on bacterial replication origins (Part III); studies on replication origins of bacterial phages and plasmids (Part IV); studies on eukaryotic replication origins (Part V); studies on prokaryotic replication enzymology (Part VI); studies on eukaryotic replication enzymology (Part VII); studies on the fidelity of DNA replication (Part VIII); studies on DNA topoisomerases (Part IX); and studies of genetic recombination mechanisms (Part X).
