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Author: Mark C. Leake
Publisher: Cambridge University Press
Published: 2013-01-31
Total Pages: 291
ISBN-13: 1107005833
DOWNLOAD EBOOKIndispensable textbook for undergraduate students in the physical and life sciences, unravelling the inner workings of the cell.
Author: Krishnarao Appasani
Publisher: Cambridge University Press
Published: 2022-05-26
Total Pages: 171
ISBN-13: 1108423361
DOWNLOAD EBOOKA comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.
Author: Astrid Graslund
Publisher: Springer Science & Business Media
Published: 2010-04-17
Total Pages: 570
ISBN-13: 3642025986
DOWNLOAD EBOOKWritten by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
Author: Paul R. Selvin
Publisher: CSHL Press
Published: 2008
Total Pages: 511
ISBN-13: 087969775X
DOWNLOAD EBOOKGeared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.
Author: Gregory I. Mashanov
Publisher: Humana Press
Published: 2011-08-04
Total Pages: 294
ISBN-13: 9781617792601
DOWNLOAD EBOOKThe last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.
Author:
Publisher:
Published: 2002
Total Pages: 0
ISBN-13: 9780815332183
DOWNLOAD EBOOKAuthor: Alexander E. Knight
Publisher: Academic Press
Published: 2009-02-26
Total Pages: 369
ISBN-13: 008092123X
DOWNLOAD EBOOKSingle molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. - Organized by biological system or molecule - Each chapter discusses insights into mechanism of action, structure, and function - Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc - Includes an introduction to key principles and an historical overview - Discusses applications in molecular diagnostics and drug discovery - Provides an expert's perspective on future development
Author: Nathan R. Zaccai
Publisher: Cambridge University Press
Published: 2017-05-18
Total Pages: 710
ISBN-13: 1108508804
DOWNLOAD EBOOKCurrent techniques for studying biological macromolecules and their interactions are based on the application of physical methods, ranging from classical thermodynamics to more recently developed techniques for the detection and manipulation of single molecules. Reflecting the advances made in biophysics research over the past decade, and now including a new section on medical imaging, this new edition describes the physical methods used in modern biology. All key techniques are covered, including mass spectrometry, hydrodynamics, microscopy and imaging, diffraction and spectroscopy, electron microscopy, molecular dynamics simulations and nuclear magnetic resonance. Each method is explained in detail using examples of real-world applications. Short asides are provided throughout to ensure that explanations are accessible to life scientists, physicists and those with medical backgrounds. The book remains an unparalleled and comprehensive resource for graduate students of biophysics and medical physics in science and medical schools, as well as for research scientists looking for an introduction to techniques from across this interdisciplinary field.
Author: R. Rigler
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 503
ISBN-13: 3642595421
DOWNLOAD EBOOKThis is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.
Author: R. Rigler
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 375
ISBN-13: 3642565441
DOWNLOAD EBOOKThe topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
