Single Molecule and Single Cell Sequencing
Single Molecule and Single Cell Sequencing

Author: Yutaka Suzuki

Publisher: Springer

Published: 2019-04-09

Total Pages: 150

ISBN-13: 9811360375

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This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians.

Single Cell Analysis
Single Cell Analysis

Author: Dario Anselmetti

Publisher: John Wiley & Sons

Published: 2009-03-09

Total Pages: 284

ISBN-13: 3527626654

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The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.

Microfluidics for Single-Cell Analysis
Microfluidics for Single-Cell Analysis

Author: Jin-Ming Lin

Publisher: Springer Nature

Published: 2019-08-28

Total Pages: 261

ISBN-13: 9813297298

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This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Single Molecule Analysis
Single Molecule Analysis

Author: Iddo Heller

Publisher: Springer Nature

Published: 2023-11-13

Total Pages: 511

ISBN-13: 1071633775

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This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Single Molecule Biology
Single Molecule Biology

Author: Alexander E. Knight

Publisher: Academic Press

Published: 2009-02-26

Total Pages: 369

ISBN-13: 008092123X

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Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective.The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. Organized by biological system or molecule Each chapter discusses insights into mechanism of action, structure, and function Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc Includes an introduction to key principles and an historical overview Discusses applications in molecular diagnostics and drug discovery Provides an expert's perspective on future development

Advances in Single Molecule, Real-Time (SMRT) Sequencing
Advances in Single Molecule, Real-Time (SMRT) Sequencing

Author: Adam Ameur

Publisher: MDPI

Published: 2019-11-18

Total Pages: 128

ISBN-13: 3039217003

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PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.

Single Cell Metabolism
Single Cell Metabolism

Author: Bindesh Shrestha

Publisher: Humana

Published: 2019-09-30

Total Pages: 0

ISBN-13: 9781493998296

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This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field.

Precision Medicine for Investigators, Practitioners and Providers
Precision Medicine for Investigators, Practitioners and Providers

Author: Joel Faintuch

Publisher: Academic Press

Published: 2019-11-16

Total Pages: 640

ISBN-13: 0128191791

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Precision Medicine for Investigators, Practitioners and Providers addresses the needs of investigators by covering the topic as an umbrella concept, from new drug trials to wearable diagnostic devices, and from pediatrics to psychiatry in a manner that is up-to-date and authoritative. Sections include broad coverage of concerning disease groups and ancillary information about techniques, resources and consequences. Moreover, each chapter follows a structured blueprint, so that multiple, essential items are not overlooked. Instead of simply concentrating on a limited number of extensive and pedantic coverages, scholarly diagrams are also included. Provides a three-pronged approach to precision medicine that is focused on investigators, practitioners and healthcare providers Covers disease groups and ancillary information about techniques, resources and consequences Follows a structured blueprint, ensuring essential chapters items are not overlooked

Highly Multiplexed Single Cell in Situ Transcriptomic Analysis
Highly Multiplexed Single Cell in Situ Transcriptomic Analysis

Author: Lu Xiao (Ph.D.)

Publisher:

Published: 2019

Total Pages: 104

ISBN-13:

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Spatial resolved detection and quantification of ribonucleic acid (RNA) molecules in single cell is crucial for the understanding of inherent biological issues, like mechanism of gene regulation or the development and maintenance of cell fate. Conventional methods for single cell RNA profiling, like single-cell RNA sequencing (scRNA-seq) or single-molecule fluorescent in situ hybridization (smFISH), suffer either from the loss of spatial information or the low detection throughput. In order to advance single-cell analysis, new approaches need to be developed with the ability to perform high-throughput detection while preserving spatial information of the subcellular location of target RNA molecules. Novel approaches for highly multiplexed single cell in situ transcriptomic analysis were developed by our group to enable single-cell comprehensive RNA profiling in their native spatial contexts. Reiterative FISH was demonstrated to be able to detect >100 RNA species in single cell in situ, while more sophisticated approaches, consecutive FISH (C-FISH) and switchable fluorescent oligonucleotide based FISH (SFO-FISH), have the potential for whole transcriptome profiling at the single molecule sensitivity. The introduction of a cleavable fluorescent tyramide even enables sensitive RNA profiling in intact tissues with high throughput. These approaches will have wide applications in studies of systems biology, molecular diagnosis and targeted therapies.