RNA Polymerase and Associated Factors, Part D
RNA Polymerase and Associated Factors, Part D

Author: Sankar Adhya

Publisher: Elsevier

Published: 2003-12-18

Total Pages: 715

ISBN-13: 0080522602

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RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.* Models and methods for studying polymerase translocation* Assay for movements of RNA polymerase along DNA* Engineering of elongation complexes of bacterial and yeast RNA polymerases

RNA Polymerase and Associated Factors, Part C
RNA Polymerase and Associated Factors, Part C

Author: Sankar Adhya

Publisher: Elsevier

Published: 2003-12-18

Total Pages: 833

ISBN-13: 0080522599

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RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.* Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors

Liposomes, Part D
Liposomes, Part D

Author:

Publisher: Elsevier

Published: 2004-07-17

Total Pages: 379

ISBN-13: 0080522610

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Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part D is a continuation of previous Methods in Enzymology Liposome volumes A, B, and C. - Covers antibody or ligand targeted liposomes; environment sensitive liposomes; liposomal oligonucleotides; liposomes in vivo

Numerical Computer Methods, Part D
Numerical Computer Methods, Part D

Author:

Publisher: Elsevier

Published: 2004-05-25

Total Pages: 527

ISBN-13: 0080497217

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The aim of Numerical Computer Methods, Part D is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected. Selected Contents: Prediction of protein structure Modeling and studying proteins with molecular dynamics Statistical error in isothermal titration calorimetry Analysis of circular dichroism data Model comparison methods

Nitric Oxide, Part D
Nitric Oxide, Part D

Author:

Publisher: Elsevier

Published: 2002-12-09

Total Pages: 553

ISBN-13: 0080497039

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Presents information on nitrosothiols and nitric oxide in cell signaling - Details nitric oxide and mitochondrial functions - Explains nitric oxide synthases

Energetics of Biological Macromolecules, Part D
Energetics of Biological Macromolecules, Part D

Author: Jo M. Holt

Publisher: Elsevier

Published: 2004-04-02

Total Pages: 315

ISBN-13: 0080497179

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This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed.*Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis

Biothermodynamics, Part D
Biothermodynamics, Part D

Author:

Publisher: Academic Press

Published: 2011-02-16

Total Pages: 377

ISBN-13: 0123860040

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The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. - Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules - Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research - Offers step-by-step lab instructions, including necessary equipment, from a global research community

Regulators and Effectors of Small GTPases, Part D: Rho Family
Regulators and Effectors of Small GTPases, Part D: Rho Family

Author:

Publisher: Elsevier

Published: 2000-10-11

Total Pages: 583

ISBN-13: 0080496806

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This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Nuclear Magnetic Resonance of Biological Macromolecules, Part A
Nuclear Magnetic Resonance of Biological Macromolecules, Part A

Author: Thomas L. James

Publisher: Academic Press

Published: 2001

Total Pages: 528

ISBN-13:

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This volume and its companion, Volume 339, supplement Volumes 176, 177, 239, and 261. Chapters are written with a "hands-on" perspective. That is, practical applications with critical evaluations of methodologies and experimental considerations needed to design, execute, and interpret NMR experiments pertinent to biological molecules.