Rice Protocols

Rice Protocols

Author: Yinong Yang

Publisher: Humana Press

Published: 2012-11-08

Total Pages: 0

ISBN-13: 9781627031936

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With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of “omics” techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.


Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

Author: Fatma Sarsu

Publisher: Springer

Published: 2018-08-09

Total Pages: 51

ISBN-13: 3319773380

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This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance.


Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley

Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley

Author: Souleymane Bado

Publisher: Springer

Published: 2016-02-22

Total Pages: 44

ISBN-13: 3319265903

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This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and barley seedlings, which uses hydroponics. The protocols are devised for use by plant breeders and can be easily accommodated into breeding practice.


Rice Genome Engineering and Gene Editing

Rice Genome Engineering and Gene Editing

Author: Anindya Bandyopadhyay

Publisher:

Published: 2021

Total Pages:

ISBN-13: 9781071610688

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This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.


Rice Grain Quality

Rice Grain Quality

Author: Nese Sreenivasulu

Publisher: Humana

Published: 2019-12-10

Total Pages: 338

ISBN-13: 9781493994106

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This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germplasm. The chapters in this book cover topics such as seed development stages; panicle architectural traits to understand yield components; starch structure and protein properties; and genome editing technologies to reveal grain quality and nutritional traits. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rice Grain Quality: Methods and Protocols is a valuable resource for all researchers interested in expanding their knowledge in this evolving field.


Agrobacterium Protocols

Agrobacterium Protocols

Author: Kan Wang

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 474

ISBN-13: 1597451304

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Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.


Phenotyping protocols for abiotic stress tolerance in rice

Phenotyping protocols for abiotic stress tolerance in rice

Author: IRRI

Publisher: IRRI

Published:

Total Pages: 72

ISBN-13:

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In this manual, we provide detailed protocols for screening rice under the major stresses affecting both rainfed and irrigated rice: submergence, salinity, drought, and the soil problems – excess iron and aluminum. We also include protocols for a new area of abiotic stress research since it is increasingly occurring in farmers’ field due to climate change: combined stresses. Results from these protocols on combined drought + salinity and submergence + salinity indicate that the effects on the rice plant are sometimes different than under individual stresses. The protocols in this manual have been developed and optimized over decades at IRRI. Numerous scientists, technical staff and students have been involved in the development and use of these protocols, and it is often their experiences that have led to improvements, all of which are highly acknowledged. Our objective with this compilation is to provide a resource for researchers around the globe who aim to improve the abiotic stress tolerance of rice as part of the effort to benefit rice farmers in both rainfed and irrigated areas affected by abiotic stresses


Doubled Haploid Production in Crop Plants

Doubled Haploid Production in Crop Plants

Author: M. Maluszynski

Publisher: Springer Science & Business Media

Published: 2013-06-29

Total Pages: 451

ISBN-13: 940171293X

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The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes. This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for many crops including some woody tree species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations. For many years, both the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) have supported and coordinated research that focuses on development of more efficient doubled haploid production methods and their applications in breeding of new varieties and basic research through their Plant Breeding and Genetics Section of the Joint F AO/IAEA Division of Nuclear Techniques in Food and Agriculture. The first F AO/IAEA scientific network (Coordinated Research Programme - CRP) dealing with doubled haploids was initiated by the Plant Breeding and Genetics Section in 1986.


Iced!

Iced!

Author: Gary R. Reinl

Publisher:

Published: 2013-10-14

Total Pages: 165

ISBN-13: 9780989831918

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The Rice Diet Solution

The Rice Diet Solution

Author: Kitty Gurkin Rosati

Publisher: Simon and Schuster

Published: 2006-06-06

Total Pages: 369

ISBN-13: 1416537899

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Can you really lose twenty pounds in a month? Will you really keep it off this time? With The Rice Diet Solution, you will! The Rice Diet Program has been helping dieters successfully lose weight since 1939. Now in book form, this world-renowned weight-loss method can help you change the way you eat forever. The Rice Diet Program in Durham, North Carolina, was one of the first medical facilities in America to use diet as the primary way to treat disease. On this high-complex-carb, low-fat, and low-sodium whole-foods diet, “Ricers” lose weight faster, more safely, and more effectively than people on any other diet. Men lose on average twenty-eight to thirty pounds and women on average nineteen to twenty pounds per month! The Rice Diet also detoxes your body, ridding it of excess water weight and toxins from processed foods and the environment. The program's results have been documented by extensive studies and confirmed by thousands of people who report amazing weight loss, as well as immediate improvement in such conditions as heart disease, diabetes, and hypertension. Here’s how it works: The Rice Diet strictly limits salt and sodium-rich ingredients. Salt, like refined sugar, is an appetite stimulant, so when you reduce salt intake, you lose water weight and are less inclined to overeat. The Rice Diet also limits saturated fats and instead relies on carbohydrates (fruits, vegetables, grains, and beans) as the main source of nutrition. The fiber cleanses your system and satisfies you so you feel full quickly. The Rice Diet makes it easy to limit calories; when you’re eating foods that truly satisfy your hunger, it’s a challenge to eat 1,500 calories per day! To make it easy to follow the program, The Rice Diet Solution includes hundreds of tasty, filling, easy-to-prepare recipes—some from the Rice House kitchen, others inspired by major chefs and adapted to Rice Diet standards.