Regulation of Accessibility of the Variable Gene Segments of the Mouse Immunoglobulin Kappa Light Chain Gene Locus
Author: Katherine Meyers Brekke
Publisher:
Published: 2005
Total Pages:
ISBN-13:
DOWNLOAD EBOOKRead and Download eBook Full
Author: Katherine Meyers Brekke
Publisher:
Published: 2005
Total Pages:
ISBN-13:
DOWNLOAD EBOOKAuthor: Kenneth Murphy
Publisher: Garland Science
Published: 2010-06-22
Total Pages:
ISBN-13: 9780815344575
DOWNLOAD EBOOKThe Janeway's Immunobiology CD-ROM, Immunobiology Interactive, is included with each book, and can be purchased separately. It contains animations and videos with voiceover narration, as well as the figures from the text for presentation purposes.
Author: Ruth Epstein
Publisher:
Published: 1984
Total Pages: 420
ISBN-13:
DOWNLOAD EBOOKAuthor: Alan Murray Shapiro
Publisher:
Published: 1993
Total Pages: 208
ISBN-13:
DOWNLOAD EBOOKAuthor: Claire Ward
Publisher:
Published: 2017
Total Pages:
ISBN-13:
DOWNLOAD EBOOKImmunoglobulins are receptors expressed on the outside of a B cell that can specifically bind pathogens and toxic substances within a host. These receptors are heterodimers of two chains: heavy and light, which are encoded at separate loci. Enzymatic splicing of gene segments at heavy and light chain loci within the genomic DNA in every B cell results in a highly diversified and specific repertoire of immunoglobulins in a single host. Spaceflight is known to affect reduce splenic B cell populations and B cell progenitors within the bone marrow, potentially restricting the diversity of the immunoglobulin repertoire (Ig-Rep). The objective of this thesis project was to characterize the impact of spaceflight on the kappa light-chain Ig-Rep of the C57BL/6 mouse. High-throughput sequencing (HTS) technologies have enabled the rapid characterization of Ig-Reps, however, standard Ig-Rep workflows often rely the amplification of immunoglobulin sequences to ensure the capture immunoglobulin sequences from rare B cell clones. Additionally, the Ig-Rep is often assessed in sorted B cell populations. Opportunities for spaceflight experiments are limited and costly, and the exclusive amplification of immunoglobulin sequences prior to HTS results in a dataset that cannot be mined for additional information. Furthermore, due to the difficulties of tissue collection in spaceflight, HTS of sorted B cell populations is not feasible. We optimized a protocol in which the Ig-Rep was assessed from unamplified whole tissue immunoglobulin transcripts. The Ig-Rep was characterized by gene segment usage, gene segment combinations and the region in which gene segments are joined. HTS datasets of ground control animals and animals flown aboard the International Space Station were compared to explore the impact of spaceflight on the unimmunized murine Ig-Rep.
Author: Xiangdong Liu
Publisher:
Published: 1998
Total Pages: 346
ISBN-13:
DOWNLOAD EBOOKAuthor: Matthew Arthur Kalani Inlay
Publisher:
Published: 2003
Total Pages: 346
ISBN-13:
DOWNLOAD EBOOKAuthor: Michael Charles Komaromy
Publisher:
Published: 1983
Total Pages: 240
ISBN-13:
DOWNLOAD EBOOKAuthor: LARRY WAYNE TJOELKER
Publisher:
Published: 1991
Total Pages: 350
ISBN-13:
DOWNLOAD EBOOKsegments. Both processes are activated before exposure of the duck to exogeneous antigen and therefore contribute to the primary IgL repertoire. The Muscovy duck IgL locus has provided new insight into the molecular mechanisms and regulation of V-J recombination and gene conversion.