Reduction in the Numbers of Antibody-Producing Cells in Rainbow Trout, Oncorhynchus Mykiss, Exposed to Sublethal Doses of Phenol Before Bath Immunization
Author: DP. Anderson
Publisher:
Published: 1990
Total Pages: 7
ISBN-13:
DOWNLOAD EBOOKThe numbers of splenic antibody-producing cells were reduced in groups of fish given a 2, 5, or 30 min bath exposure to 10 ppm phenol before immunization by bath with the bacterin, Yersinia ruckeri O-antigen. The lethal time of 50% (LT50) for the 10 ppm phenol bath was 45 min. The passive hemolytic plaque assay was used to monitor the numbers of antibody-producing cells in the phenol-pretreated and sham-treated fish 14 or 21 days after contaminant exposure and 5 min bath immunizations at 10 or 100 ?g/mL. These results, documenting a suppression of the immune response to bath immunization following a brief exposure to a toxicant, may help to explain delayed mortalities where pollution is implicated.