Purification and Characterization of Therapeutic Protein
Purification and Characterization of Therapeutic Protein

Author: L. Premraj

Publisher: Independent Author

Published: 2023-04-10

Total Pages: 0

ISBN-13: 9781805281573

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The research paper titled "Purification and Characterization of Therapeutic Protein" authored by L. Premraj explores the purification and structural characterization of a therapeutic protein obtained from the haemolymph of the marine crab Grapsus albolineatus Milbert 1812. The paper highlights the importance of protein purification and structural characterization in the development of biotherapeutics and biopharmaceuticals. Protein purification is a critical step in the development of biotherapeutics and biopharmaceuticals. In this study, the authors have utilized various techniques such as chromatography, electrophoresis, and mass spectrometry for the purification and structural characterization of the therapeutic protein obtained from the haemolymph of the marine crab Grapsus albolineatus. The purified protein was characterized using various techniques, including amino acid sequencing, post-translational modification analysis, and biophysical and biochemical analyses. The molecular weight, glycosylation, protein folding, and conformational analysis of the protein were also studied. The results of the characterization showed that the protein has high biological activity and binding affinity, making it suitable for drug development. The study also highlights the importance of formulation development, quality control, batch-to-batch consistency, and stability analysis in the development of biotherapeutics and biopharmaceuticals. The therapeutic efficacy, pharmacokinetics, pharmacodynamics, immunogenicity, and toxicity evaluation of the protein were also studied in preclinical and clinical trials. Moreover, the research emphasizes the importance of regulatory compliance and good manufacturing practices in the bioprocessing and downstream processing of the protein. The study also explores the use of protein engineering and recombinant protein technology for the development of biotherapeutics and biopharmaceuticals. In conclusion, this research paper provides valuable insights into the purification and characterization of a therapeutic protein obtained from the haemolymph of the marine crab Grapsus albolineatus. The study highlights the importance of protein purification and structural characterization in the development of biotherapeutics and biopharmaceuticals. The findings of this study will contribute to the development of novel and effective drugs for the benefit of society

Therapeutic Proteins
Therapeutic Proteins

Author: C. Mark Smales

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 481

ISBN-13: 1592599222

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With the recent completion of the sequencing of the human genome, it is widely anticipated that the number of potential new protein drugs and targets will escalate at an even greater rate than that observed in recent years. However, identification of a potential target is only part of the process in developing these new next generation protein-based “drugs” that are increasingly being used to treat human disease. Once a potential protein drug has been identified, the next rate-limiting step on the road to development is the production of sufficient authentic material for testing, charact- ization, clinical trials, and so on. If a protein drug does actually make it through this lengthy and costly process, methodology that allows the production of the protein on a scale large enough to meet demand must be implemented. Furthermore, large-scale production must not compromise the authenticity of the final product. It is also nec- sary to have robust methods for the purification, characterization, viral inactivation and continued testing of the authenticity of the final protein product and to be able to formulate it in a manner that retains both its biological activity and lends itself to easy administration. Therapeutic Proteins: Methods and Protocols covers all aspects of protein drug production downstream of the discovery stage. This volume contains contributions from leaders in the field of therapeutic protein expression, purification, characterization, f- mulation, and viral inactivation.

Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics
Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics

Author: Allan Matte

Publisher: Elsevier

Published: 2020-09-07

Total Pages: 222

ISBN-13: 0081030193

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Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography

Principles and Reactions of Protein Extraction, Purification, and Characterization
Principles and Reactions of Protein Extraction, Purification, and Characterization

Author: Hafiz Ahmed

Publisher: CRC Press

Published: 2017-07-27

Total Pages: 408

ISBN-13: 0203507436

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Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different

Therapeutic Proteins
Therapeutic Proteins

Author: Vladimir Voynov

Publisher: Humana Press

Published: 2016-05-01

Total Pages: 516

ISBN-13: 9781493959495

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Emphasizing the newest developments in the field, this volume presents detailed methodswith added emphasison therapeutic protein discovery. It features key tips and valuable implementation advice to ensure successful results."

Purification and Analysis of Recombinant Proteins
Purification and Analysis of Recombinant Proteins

Author: Ramnath Seetharam

Publisher: CRC Press

Published: 1991-01-07

Total Pages: 352

ISBN-13: 9780824782771

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Covering both new and traditional topics in the purification and analysis of recombinant proteins, this volume demonstrates how to overcome problems in protein research and presents practical methods used in protein work, explaining their theoretical bases. The collection also explores innovative co

Analytical Characterization of Biotherapeutics
Analytical Characterization of Biotherapeutics

Author: Jennie R. Lill

Publisher: John Wiley & Sons

Published: 2017-08-14

Total Pages: 378

ISBN-13: 1119053102

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The definitive guide to the myriad analytical techniques available to scientists involved in biotherapeutics research Analytical Characterization of Biotherapeutics covers all current and emerging analytical tools and techniques used for the characterization of therapeutic proteins and antigen reagents. From basic recombinant antigen and antibody characterization, to complex analyses for increasingly complex molecular designs, the book explores the history of the analysis techniques and offers valuable insights into the most important emerging analytical solutions. In addition, it frames critical questions warranting attention in the design and delivery of a therapeutic protein, exposes analytical challenges that may occur when characterizing these molecules, and presents a number of tested solutions. The first single-volume guide of its kind, Analytical Characterization of Biotherapeutics brings together contributions from scientists at the leading edge of biotherapeutics research and manufacturing. Key topics covered in-depth include the structural characterization of recombinant proteins and antibodies, antibody de novo sequencing, characterization of antibody drug conjugates, characterization of bi-specific or other hybrid molecules, characterization of manufacturing host-cell contaminant proteins, analytical tools for biologics molecular assessment, and more. Each chapter is written by a recognized expert or experts in their field who discuss current and cutting edge approaches to fully characterizing biotherapeutic proteins and antigen reagents Covers the full range of characterization strategies for large molecule based therapeutics Provides an up-to-date account of the latest approaches used for large molecule characterization Chapters cover the background needed to understand the challenges at hand, solutions to characterize these large molecules, and a summary of emerging options for analytical characterization Analytical Characterization of Biotherapeutics is an up-to-date resource for analytical scientists, biologists, and mass spectrometrists involved in the analysis of biomolecules, as well as scientists employed in the pharmaceuticals and biotechnology industries. Graduate students in biology and analytical science, and their instructors will find it to be fascinating and instructive supplementary reading.

PEGylated Protein Drugs: Basic Science and Clinical Applications
PEGylated Protein Drugs: Basic Science and Clinical Applications

Author: Francesco M. Veronese

Publisher: Springer Science & Business Media

Published: 2009-12-30

Total Pages: 289

ISBN-13: 3764386797

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PEGylation technology and key applications are introduced by this topical volume. Basic physical and chemical properties of PEG as basis for altering/improving in vivo behaviour of PEG-conjugates such as increased stability, improved PK/PD, and decreased immunogenicity, are discussed. Furthermore, chemical and enzymatic strategies for the coupling and the conjugate characterization are reported. Following chapters describe approved and marketed PEG-proteins and PEG-oligonucleotides as well as conjugates in various stages of clinical development.

Protein Analysis and Purification
Protein Analysis and Purification

Author: I.M. Rosenberg

Publisher: Springer Science & Business Media

Published: 2013-03-14

Total Pages: 453

ISBN-13: 1475711085

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This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip ment other than encouragement to read the manual, if you can find it. Through out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.