Protein Trafficking Along the Exocytotic Pathway
Author: Wanjin Hong
Publisher:
Published: 1996
Total Pages: 197
ISBN-13: 9781570593499
DOWNLOAD EBOOKRead and Download eBook Full
Author: Wanjin Hong
Publisher:
Published: 1996
Total Pages: 197
ISBN-13: 9781570593499
DOWNLOAD EBOOKAuthor: Nava Segev
Publisher: Springer Science & Business Media
Published: 2010-05-30
Total Pages: 459
ISBN-13: 038793877X
DOWNLOAD EBOOKThis book covers the past, present and future of the intra-cellular trafficking field, which has made a quantum leap in the last few decades. It details how the field has developed and evolved as well as examines future directions.
Author: Anne Müsch
Publisher: Biota Publishing
Published: 2014-09-01
Total Pages: 136
ISBN-13: 1615046593
DOWNLOAD EBOOKThe concept of vesicular traffic as a means of protein transport in and out of cells and between membrane compartments has been established since the 1960s. Its basic principles are beautifully simple, yet the details of protein transport are complex enough to find novel classes of transport vesicles, and trafficking itineraries still being described to date. In this treatise, the reader will be introduced to mechanisms of vesicle sculpting, cargo selection, vesicle targeting, and vesicle consumption that have emerged as common characteristics of multiple transport steps in the exocytic and endocytic pathways. These fundamentals also reveal the basis for the specificity and selectivity of individual transport steps. We will further discuss how protein transport might lead to the establishment and maintenance of the endomembrane system of eukaryotic cells. The concepts for these mechanisms are based on experimental evidence combined with mathematical modeling that can disclose the minimal requirements for the generation of the distinct membrane compartments. Table of Contents: Compartmentalization Is a Hallmark of Eukaryotic Cells / Organelles Can Be Classified Based on Their Origin / There Are Multiple Entry Points Into the Endomembrane System and a Network of Connecting Trafficking Routes / There Are Exceptions and Extensions to the Classic Trafficking Itineraries / A Mathematical Model Can Explain How Organelle Size Is Established and Maintained / Coats and SNAREs Constitute the Core Machinery for Vesicle Budding and Fusion / A Mathematical Model Explains How Coats and SNAREs Are Sufficient to Generate Organelle Identity / How Vesicle Formation Is Linked to Cargo Incorporation / RabGTPases Are the Master Regulators of Vesicular Traffic / The Mechanisms and Physiological Roles of Endocytosis / Models for Protein Transport in the Golgi Apparatus / Summary / Acknowledgments / References / Author Biography
Author:
Publisher:
Published: 2002
Total Pages: 0
ISBN-13: 9780815332183
DOWNLOAD EBOOKAuthor: Andrei I. Ivanov
Publisher: Springer Science & Business Media
Published: 2008
Total Pages: 407
ISBN-13: 1588298655
DOWNLOAD EBOOKIn this book, skilled experts provide the most up-to-date, step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. The book is insightful to both newcomers and seasoned professionals. It offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.
Author: Jingshi Shen
Publisher: Springer Nature
Published: 2022-07-12
Total Pages: 408
ISBN-13: 1071622099
DOWNLOAD EBOOKThis detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Trafficking: Methods and Protocols provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.
Author: Richard A. Kahn
Publisher: Springer Science & Business Media
Published: 2004-02-29
Total Pages: 368
ISBN-13: 1402017197
DOWNLOAD EBOOKFor the first time experts in the area of signalling research with a focus on the ARF family have contributed to the production of a title devoted to ARF biology. A comprehensive phylogenetic analysis of the ARF family, tables of the ARF GEFs and ARF GAPs, and more than a dozen chapters describing them in detail are provided. The impact of the ARF proteins on widely diverse aspects of cell biology and cell signalling can be clearly seen from the activities described; including membrane traffic, lipid metabolism, receptor desensitization, mouse development, microtubule dynamics, and bacterial pathogenesis. Anyone interested in understanding the complexities of cell signalling and the integration of signalling networks will benefit from this volume.
Author: Wanjin Hong
Publisher:
Published: 1996
Total Pages: 216
ISBN-13:
DOWNLOAD EBOOKAuthor: An Rykx
Publisher:
Published: 2007-05
Total Pages: 0
ISBN-13: 9789058676061
DOWNLOAD EBOOKAuthor: Martin Chalfie
Publisher: John Wiley & Sons
Published: 2005-11-18
Total Pages: 486
ISBN-13: 0471739480
DOWNLOAD EBOOKSince the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.