Authored by an integrated committee of plant and animal scientists, this review of newer molecular genetic techniques and traditional research methods is presented as a compilation of high-reward opportunities for agricultural research. Directed to the Agricultural Research Service and the agricultural research community at large, the volume discusses biosciences research in genetic engineering, animal science, plant science, and plant diseases and insect pests. An optimal climate for productive research is discussed.
This important reference/text provides technologists with the basic informationnecessary to interact scientifically with molecular biologists and get involved in scalinguplaboratory procedures and designing and constructing commercial plants.Requiring no previous training or experience in biology, Genetic EngineeringFundamentals explains the biological and chemical principles of recombinant DNAtechnology ... emphasizes techniques used to isolate and clone specific genes frombacteria, plants, and animals, and methods of scaling-up the formation of the geneproduct for commercial applications ... analyzes problems encountered in scaling-upthe microprocessing of biochemical procedures . .. includes an extensive glossary andnumerous illustrations ... identifies other resource materials in the field ... and more.Presenting the fundamentals of biochemistry and molecular biology to workers andstudents in other fields, this state-of-the-art reference/text is essentiai reading fortechnologists in chemistry and engineering; biomedical, chemical, electrical andelectronics, industrial, mechanical, manufacturing, design, plant, control, civil, genetic,and environmental engineers; chemists, botanists, and zoologists; and advancedundergraduate and graduate courses in engineering, biotechnology, and industrialmicrobiology.
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.
Provides concise yet complete knowledge on the many aspects of the most useful yet the most controversial scientific techniques developed under the umbrella of genetic engineering, including detection, isolation and quantitation of DNA and RNA; enzymes of recombinant DNA technology and genome editing; cloning vectors; DNA cloning; DNA libraries; identification of gene of interest; analysis of cloned gene; plant marker gene systems; gene transfer through natural means; vectorless methods of gene transfer; gene transfer methods in plants; gene transfer methods in animals; assessment of gene transfer methods; RNA interference technology in designing transgenic crops; chloroplast genetic engineering; mitochondrial engineering; metabolic engineering; clean gene technology; genetic use restriction technology; mapping and cloning of genetic determinants of QTLs; applications and benefits of genetic engineering; Issues and disadvantages of genetic engineering; risk assessment of genetically engineered organisms; and some recent approaches, namely, genome engineering and genomically recoded organisms, currently being developed for production of safer genetically modified organisms. This book is primarily designed as a text book for undergraduate and graduate students studying genetic engineering in agricultural, veterinary and medicinal universities. Teachers and researchers in any discipline of life sciences, agricultural sciences, medicine, and biotechnology in all the conventional and agricultural universities, research institutes, molecular genetics and biotechnology companies/colleges/schools all over the world will also find it useful as a reference book.
An introductory text which provides coverage of biomolecular structure, function, metabolism, and molecular biology with major emphasis on three-dimensional biochemistry. Computer-generated stereo views depict the conformation of biomolecules; a free stere