Practice of Thin Layer Chromatography

Practice of Thin Layer Chromatography

Author: Joseph C. Touchstone

Publisher:

Published: 1983-03

Total Pages: 430

ISBN-13:

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A practical how-to guide to all the basic techniques needed to practice thin layer chromatography in biochemical/pharmaceutical research and quality control. This updated edition presents the most current techniques as well as the hows and whys of TLC. Provides step-by-step methods for performing the separations as well as doing related tasks, such as applying the sample, selecting the mobile phase, and quantitation. Includes a special chapter on how to select solvents for the development of a chromatogram to separate specific individual components of a mixture.


Applied Thin-Layer Chromatography

Applied Thin-Layer Chromatography

Author: Elke Hahn-Deinstrop

Publisher: John Wiley & Sons

Published: 2007-02-27

Total Pages: 330

ISBN-13: 3527609857

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Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition of the practical TLC guide features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced. Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures. How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.


Practice of Thin Layer Chromatography

Practice of Thin Layer Chromatography

Author: Joseph C. Touchstone

Publisher: John Wiley & Sons

Published: 1992-08-04

Total Pages: 406

ISBN-13: 9780471612223

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This Third Edition provides all the basic applications needed to practice thin layer chromatography (TLC). New material includes: the latest techniques on sample preparation and zone detection, the hybridization of TLC with high performance liquid chromatography (HPLC) as it's been developing in the last few years, emphasis on numerous applications of HPTLC involving pharmaceuticals and drugs, plus the fundamental studies of mechanisms, theories and the optimization of TLC.


Practical Thin-Layer Chromatography

Practical Thin-Layer Chromatography

Author: Bernard Fried

Publisher: CRC Press

Published: 2017-11-22

Total Pages: 294

ISBN-13: 1351422413

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Practical Thin-Layer Chromatography provides thorough coverage of the principles, practices, and applications of thin-layer chromatography (TLC) for important sample and compound types. This information is directed specifically at workers in the most active scientific fields.


Instrumental Thin-Layer Chromatography

Instrumental Thin-Layer Chromatography

Author: Colin Poole

Publisher: Elsevier

Published: 2023-01-14

Total Pages: 692

ISBN-13: 0323983014

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Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques. This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography. - Features individual chapters written by recognized authoritative and visionary experts in the field - Provides an overview and focused treatment of a single topic - Provides tables and diagrams with commonly used data to facilitate practical work, comparison of results, and decision-making - Places modern developments in the research literature into a general context not always apparent to inexperienced users of the technique - Offers comprehensive updates to all chapters - Includes new chapters on instrument platforms, effects-directed detection, data analysis tools, small-scale and microfluidic planar separation systems, and applications to the separation of amino acids and peptides, the analysis of saccharides and lipids, and forensic analysis


Thin-Layer Chromatography

Thin-Layer Chromatography

Author: Peter E Wall

Publisher: Royal Society of Chemistry

Published: 2007-10-31

Total Pages: 195

ISBN-13: 1847552463

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Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures and pitfalls on the practical application of TLC. Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.


Handbook of Thin-Layer Chromatography

Handbook of Thin-Layer Chromatography

Author: Joseph Sherma

Publisher: CRC Press

Published: 2003-04-18

Total Pages: 1356

ISBN-13: 0824748662

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In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.


High-Performance Thin-Layer Chromatography (HPTLC)

High-Performance Thin-Layer Chromatography (HPTLC)

Author: ManMohan Srivastava

Publisher: Springer Science & Business Media

Published: 2010-11-15

Total Pages: 401

ISBN-13: 3642140254

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The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.


Quantitative Thin-Layer Chromatography

Quantitative Thin-Layer Chromatography

Author: Bernd Spangenberg

Publisher: Springer Science & Business Media

Published: 2011-01-03

Total Pages: 399

ISBN-13: 364210729X

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Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.


Techniques and Practice of Chromatography

Techniques and Practice of Chromatography

Author: Raymond P.W. Scott

Publisher: CRC Press

Published: 2020-01-29

Total Pages: 426

ISBN-13: 1000723615

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This work introduces scientists of all disciplines to the chromatographic process and how it functions. The basic principles of chromatographic separation and specific chromatographic procedures, including gas, liquid and thin-layer chromatography, are covered. For each separation method the book details its characteristics, the instrumentation required, the procedures necessary for effective use, areas of application and examples of its use.;This work is intended for analytical chemists, laboratory technicians, and upper-level undergraduate and graduate students in analytical chemistry or separation science courses.