In recent years there has been a growing awareness of the importance of reproductive biology to crop production and there has been a tremendous increase in research on reproductive structures of higher plants. Presented here is a wide information of different aspects of micro- and macrosporogenesis, pollen-stigma interaction and recognition, pollen tube growth, cytoskeleton, in vitro and in vivo gamete fusion, and incompatibility. The most advanced techniques employed in studies on reproductive biology of higher plants are described in detail.
The subject of this volume is the reproductive biology of plants. A steadily growing interest in this field is the result of at least two factors, as pointed out with great foresight by one of the driving forces in the field, H.E Linskens (Linskens 1964): most of the food consumed by humans takes the form of plant reproductive parts, and molecular biology now provides pow erful tools for investigating and manipulating plant reproductive systems. Molecular biology and the allied discipline of biotechnology are solidly represented in the papers in this book. The editors of Angiosperm Pollen and Ovules believe that the chapters herein contain some of the most excit ing findings of contemporary biology, and hope that the readers of this book will share their enthusiasm. The editors express sincere and grateful thanks for help from Carla Frova, Enrico Pe, and especially to Giorgio Binelli, all of the University of Milan. Without these three tireless and enthusiastic individuals it would not have been possible to maintain the apparently effortless proceeding of this congress. We thank also the organizing committee and the organizations that generously provided financial support (both listed below). Finally, we extend thanks to M. Cresti, D. Charlesworth, D. Hess, E Hoekstra, R. Bruce Knox, J.P. Mascarenhas, M.E. Nasrallah, P.L. Pfahler, A. Snow, and M.T.M. Willemse for chairing sessions.
Biotechnological methods are opening new ways in plant breeding. They allow novel strategies for improving crop productivity and quality, especially in the agrofood sector. The molecular mechanisms underlying these biotechnological approaches are presented here. Topics included are: pollen development, pollen tube growth, macrosporogenesis and fertilization and the effects of pesticides on sexual plant reproduction. Fertilization in higher plants is a complex process consisting of two events, the fusion of the egg with one sperm cell resulting in the diploid zygote, and the fusion of embryosac nuclei with another sperm cell, leading to a triploid endosperm. This "double fertilization" is preceded by the pollination process and a long lasting interaction between the dipoid pistil and the haploid pollen tube (progamic phase). Fertilization of flowering plants results in the formation of seeds and fruits, our basic food supply.
Plant cell walls are complex, dynamic cellular structures essential for plant growth, development, physiology and adaptation. Plant Cell Walls provides an in depth and diverse view of the microanatomy, biosynthesis and molecular physiology of these cellular structures, both in the life of the plant and in their use for bioproducts and biofuels. Plant Cell Walls is a textbook for upper-level undergraduates and graduate students, as well as a professional-level reference book. Over 400 drawings, micrographs, and photographs provide visual insight into the latest research, as well as the uses of plant cell walls in everyday life, and their applications in biotechnology. Illustrated panels concisely review research methods and tools; a list of key terms is given at the end of each chapter; and extensive references organized by concept headings provide readers with guidance for entry into plant cell wall literature. Cell wall material is of considerable importance to the biofuel, food, timber, and pulp and paper industries as well as being a major focus of research in plant growth and sustainability that are of central interest in present day agriculture and biotechnology. The production and use of plants for biofuel and bioproducts in a time of need for responsible global carbon use requires a deep understanding of the fundamental biology of plants and their cell walls. Such an understanding will lead to improved plant processes and materials, and help provide a sustainable resource for meeting the future bioenergy and bioproduct needs of humankind.
New data on the pollen tube reflects its qualities as a biological model beyond its function as a carrier of sperm cells in plant reproduction. This book shows that pollen tubes are excellent models for plant cell research, suitable for investigations on cell tip growth and polarization, signal transduction, channel and ion flux activity, gene expression, cytoskeleton and wall structure, membrane dynamics and even cell–to-cell communication.
Plants are the basic source of food for both humans and animals. Most of the food is made of fruits and seeds. For these to be formed, pollination must first take place. This process is the transfer of pollen grains from the anther, which is the male structure of the flower, to the sigma on the female structure of the flower. The transfer process requires agents to be carried out. The agents can be either biotic or abiotic. Nature perfected this arrangement between the pollination agents and the plants. As ecosystems and agricultural systems are changing, this balanced arrangement becomes disturbed. This makes it necessary that pollination systems be studied so that necessary measures can be undertaken to ensure productivity. The chapters of this book present results in research undertaken to improve productivity in crops such as Actinidia chinensis (the kiwifruit), Theobroma cacao (cocoa), and Manicaria saccifera (a tropical forest palm). Some results are presented on tests to check the viability of pollen grains and the delivery of sperm cells through pollen tubes to the embryo sac. These results can serve as guidelines to any person seeking to improve pollination and productivity or to check the efficiency on pollination in ecosystems or agricultural production systems.
The establishment of polarity is a fundamental feature in eukaryotic development. Polarity in Plants provides an account of current research into the mechanisms by which polarity is generated at the level of the cell, organ and organism in plants, drawing especially on recent work with model organisms. The emphasis is on the use of the techniques of molecular genetics to dissect molecular mechanisms. This is the first volume to bring together the diverse aspects of polarity in plant development.
Endocytosis is a fundamental cellular process by means of which cells internalize extracellular and plasma membrane cargos for recycling or degradation. It is important for the establishment and maintenance of cell polarity, subcellular signaling and uptake of nutrients into specialized cells, but also for plant cell interactions with pathogenic and symbiotic microbes. Endocytosis starts by vesicle formation at the plasma membrane and progresses through early and late endosomal compartments. In these endosomes cargo is sorted and it is either recycled back to the plasma membrane, or degraded in the lytic vacuole. This book presents an overview of our current knowledge of endocytosis in plants with a main focus on the key molecules undergoing and regulating endocytosis. It also provides up to date methodological approaches as well as principles of protein, structural lipid, sugar and microbe internalization in plant cells. The individual chapters describe clathrin-mediated and fluid-phase endocytosis, as well as flotillin-mediated endocytosis and internalization of microbes. The book was written for a broad spectrum of readers including students, teachers and researchers.
This book contains the proceedings of the International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants, where many plant and animal reproductive biologists gathered to discuss their recent progress in investigating the shared mechanisms and factors involved in sexual reproduction. This now is the first book that reviews recent progress in almost all fields of plant and animal fertilization. It was recently reported that the self-sterile mechanism of a hermaphroditic marine invertebrate (ascidian) is very similar to the self-incompatibility system in flowering plants. It was also found that a male factor expressed in the sperm cells of flowering plants is involved in gamete fusion not only of plants but also of animals and parasites. These discoveries have led to the consideration that the core mechanisms or factors involved in sexual reproduction may be shared by animals, plants and unicellular organisms. This valuable book is highly useful for reproductive biologists as well as for biological scientists outside this field in understanding the current progress of reproductive biology.
