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Author: OECD
Publisher: OECD Publishing
Published: 2013-07-26
Total Pages: 22
ISBN-13: 9264203702
DOWNLOAD EBOOKThe test method described in this Test Guidelineis inteneded to determine the acute or letal toxicity of chemicals on embryonic stages of fish (Danio rerio). Newly fertilised zebrafish eggs are exposed to the test chemical for a period of 96 hrs ...
Author: OECD
Publisher: OECD Publishing
Published: 2019-06-18
Total Pages: 24
ISBN-13: 9264069968
DOWNLOAD EBOOKThe fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible. One or more species ...
Author: OECD
Publisher: OECD Publishing
Published: 2013-07-26
Total Pages: 24
ISBN-13: 9264203788
DOWNLOAD EBOOKThe test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to five concentrations of the test ...
Author: OECD
Publisher: OECD Publishing
Published: 1998-09-21
Total Pages: 20
ISBN-13: 9264070141
DOWNLOAD EBOOKIn this Short-term Toxicity Test on Fish Embryo and Sac-Fry Stages, the life stages from the newly fertilized egg to the end of the sac-fry stage are exposed. The embryo and sac-fry stages of fish are exposed to five concentrations of the test ...
Author: OECD
Publisher: OECD Publishing
Published: 1984-04-04
Total Pages: 9
ISBN-13: 9264069984
DOWNLOAD EBOOKThis Test Guideline presents guidance for measurement of lethal and other observed effects in fish exposed to test substances. It may be used in place of Test Guideline 203 if a longer observation period is considered useful and appropriate and the ...
Author: OECD
Publisher: OECD Publishing
Published: 2021-06-18
Total Pages: 51
ISBN-13: 9264714138
DOWNLOAD EBOOKThe RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases.
Author: OECD
Publisher: OECD Publishing
Published: 2010-07-23
Total Pages: 25
ISBN-13: 9264090894
DOWNLOAD EBOOKThis Test Guideline describes procedures designed to estimate the acute oral toxicity of substances to birds, and it provides three testing options: (1) limit dose test, (2) LD50-slope test, and (3) LD50-only test. The LD50-slope and LD50-only ...
Author: OECD
Publisher: OECD Publishing
Published: 2000-01-21
Total Pages: 16
ISBN-13: 9264070206
DOWNLOAD EBOOKThis Test Guideline is designed to assess the effects of prolonged exposure to chemicals on the growth of juvenile fish. Juvenile fish in exponential growth phase are placed, after being weighed, in test chambers and are normally exposed to five ...
Author: OECD
Publisher: OECD Publishing
Published: 2012-10-02
Total Pages: 40
ISBN-13: 9264185267
DOWNLOAD EBOOKThis Test Guideline describes an in vivo screening assay for fish reproduction where sexually mature male and spawning female fish are held together and exposed to a chemical during a limited part of their life-cycle (21 days). The short term ...
Author: OECD
Publisher: OECD Publishing
Published: 1984-04-04
Total Pages: 9
ISBN-13: 9264070044
DOWNLOAD EBOOKThis Test Guideline includes two methods: a paper contact toxicity test and an artificial soil test. The recommended specie is Eisenia foetida (Michaelsen). The initial screening test (filter paper contact test) involves exposing earthworms to test ...
