Novel insights in RNA modifications: From basic to translational research
Author: Huilin Huang
Publisher: Frontiers Media SA
Published: 2023-04-06
Total Pages: 279
ISBN-13: 2832518532
DOWNLOAD EBOOKRead and Download eBook Full
Author: Huilin Huang
Publisher: Frontiers Media SA
Published: 2023-04-06
Total Pages: 279
ISBN-13: 2832518532
DOWNLOAD EBOOKAuthor: Jian Ma
Publisher: Frontiers Media SA
Published: 2023-03-30
Total Pages: 136
ISBN-13: 283251927X
DOWNLOAD EBOOKAuthor: Kun Li
Publisher: Frontiers Media SA
Published: 2023-10-25
Total Pages: 169
ISBN-13: 2832536298
DOWNLOAD EBOOKAuthor: Alessandro Vitale
Publisher: Frontiers Media SA
Published: 2022-05-03
Total Pages: 447
ISBN-13: 2889760510
DOWNLOAD EBOOKAuthor: Leonardo Freire-de-Lima
Publisher: Frontiers Media SA
Published: 2023-04-05
Total Pages: 226
ISBN-13: 2832518486
DOWNLOAD EBOOKAuthor: Guanghua Lei
Publisher: Frontiers Media SA
Published: 2022-11-01
Total Pages: 238
ISBN-13: 288976169X
DOWNLOAD EBOOKAuthor: Morteza Jalali
Publisher: Academic Press
Published: 2017-03-31
Total Pages: 383
ISBN-13: 012803078X
DOWNLOAD EBOOKBasic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Author: Quan Cheng
Publisher: Frontiers Media SA
Published: 2023-06-08
Total Pages: 418
ISBN-13: 2832525652
DOWNLOAD EBOOKAuthor: Robert E. Rhoads
Publisher: Humana
Published: 2016-05-29
Total Pages: 0
ISBN-13: 9781493936236
DOWNLOAD EBOOKThis volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology.
Author: Institute of Medicine
Publisher: National Academies Press
Published: 2012-09-13
Total Pages: 354
ISBN-13: 0309224187
DOWNLOAD EBOOKTechnologies collectively called omics enable simultaneous measurement of an enormous number of biomolecules; for example, genomics investigates thousands of DNA sequences, and proteomics examines large numbers of proteins. Scientists are using these technologies to develop innovative tests to detect disease and to predict a patient's likelihood of responding to specific drugs. Following a recent case involving premature use of omics-based tests in cancer clinical trials at Duke University, the NCI requested that the IOM establish a committee to recommend ways to strengthen omics-based test development and evaluation. This report identifies best practices to enhance development, evaluation, and translation of omics-based tests while simultaneously reinforcing steps to ensure that these tests are appropriately assessed for scientific validity before they are used to guide patient treatment in clinical trials.