Stable Isotope Probing
Stable Isotope Probing

Author: Marc G. Dumont

Publisher: Humana

Published: 2020-08-13

Total Pages: 247

ISBN-13: 9781493997237

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This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.

Stable Isotopes in Ecology and Environmental Science
Stable Isotopes in Ecology and Environmental Science

Author: Robert Michener

Publisher: John Wiley & Sons

Published: 2008-04-15

Total Pages: 592

ISBN-13: 0470691174

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This book highlights new and emerging uses of stable isotope analysis in a variety of ecological disciplines. While the use of natural abundance isotopes in ecological research is now relatively standard, new techniques and ways of interpreting patterns are developing rapidly. The second edition of this book provides a thorough, up-to-date examination of these methods of research. As part of the Ecological Methods and Concepts series which provides the latest information on experimental techniques in ecology, this book looks at a wide range of techniques that use natural abundance isotopes to: follow whole ecosystem element cycling understand processes of soil organic matter formation follow the movement of water in whole watersheds understand the effects of pollution in both terrestrial and aquatic environments study extreme systems such as hydrothermal vents follow migrating organisms In each case, the book explains the background to the methodology, looks at the underlying principles and assumptions, and outlines the potential limitations and pitfalls. Stable Isotopes in Ecology and Environmental Science is an ideal resource for both ecologists who are new to isotopic analysis, and more experienced isotope ecologists interested in innovative techniques and pioneering new uses.

Nonisotopic DNA Probe Techniques
Nonisotopic DNA Probe Techniques

Author: Larry J. Kricka

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 373

ISBN-13: 0323137717

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Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided.This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection.Gives extensive practical informationCovers major types of nonisotopic labels and proceduresPresents background information for each methodProvides strategies and detailed experimental procedures for labeling and detecting DNA sequences byFluorescenceChemiluminescenceBioluminescenceColorimetry

Molecular Biology in Cellular Pathology
Molecular Biology in Cellular Pathology

Author: John Crocker

Publisher: John Wiley & Sons

Published: 2003-06-02

Total Pages: 428

ISBN-13: 9780470844755

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"Molecular Biology in Cellular Pathology" - Jetzt erscheint dieser erfolgreiche Klassiker in der 2. aktualisierten Auflage. Der Band beschreibt die wichtigsten Fortschritte in der Zell- und Molekularbiologie. In den letzten Jahren haben neue Erkenntnisse über den molekularen Aufbau der Zelle zur Entwicklung neuer, leistungsstarker Analysetechniken geführt, die eine größere Genauigkeit bei der Diagnose, Prognose und Behandlung von Krankheiten sowie bei der Untersuchung pathologischer Stadien ermöglichen. Insbesondere werden neue Verfahren diskutiert, die fluoreszierende Farbstoffe, DNA Microarrays, Proteinchemie und Massenspektrometrie einsetzen. Darüber hinaus enthält das Buch Informationen zum Human-Genom-Projekt und zu den neuen Disziplinen der Genomik und Proteomik, soweit diese für die Pathologie relevant sind. Neu an dieser 2. Auflage sind die Farbtafeln, die die enormen Fortschritte in der Fluoreszenz-Markierung von Zellmaterial anschaulich belegen.

Nonisotopic Probing, Blotting, and Sequencing
Nonisotopic Probing, Blotting, and Sequencing

Author: Larry J. Kricka

Publisher: Elsevier

Published: 1995-06-01

Total Pages: 539

ISBN-13: 0080537669

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Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection

PCR 3
PCR 3

Author: C. Simon Herrington

Publisher: Oxford University Press

Published: 1997-10-30

Total Pages: 223

ISBN-13: 0191565598

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PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

Zebrafish
Zebrafish

Author: Christiane Nusslein-Volhard

Publisher: Oxford University Press

Published: 2002-09-19

Total Pages: 342

ISBN-13: 9780199638086

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The zebrafish has become one of the most important model organisms to study biological processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book not only provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory. It also gives a broad overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques.

Post-translational Processing
Post-translational Processing

Author: S. J. Higgins

Publisher: OUP Oxford

Published: 1999-05-20

Total Pages: 338

ISBN-13: 0191566039

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Post - Translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding and import into organelles. The next three chapters cover the three major forms of covalent modification: phosphorylation, glycosylation, and lipid modification. Proteolytic processing the is the next topic and the final two chapters are concerned with protein turnover in mammalian cells and yeast. This book is a comprehensive volume of the best current methodology and is designed to be used at the bench or away from the bench to gain insight into future experimental approached.

Virus Culture
Virus Culture

Author: Alan Cann

Publisher: Oxford University Press

Published: 1999-07-29

Total Pages: 291

ISBN-13: 0191565849

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Virus Culture: A Practical Approach provides a broad treatment of the principles and practice of virus culture and will be of interest to all those, whether in academic, industrial, or clinical research, involved in virus culture. The first chapter is an overview of cell culture techniques essential for virologists. Other techniques then covered are isolating, identifying, concentrating, and purifying viruses. Electron Microscopy as applied to virology is also explained. Chapter 6 is about creating virus vaccines and chapters 7 and 8 cover antiserum production, monoclonal antibodies and antiviral drug testing. The final chapter describes the methods used to study plant viruses.

Protein Expression
Protein Expression

Author: S. J. Higgins

Publisher: OUP Oxford

Published: 1999-05-20

Total Pages: 303

ISBN-13: 019156558X

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Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.