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Author: Abu Bakar Salleh
Publisher: Nova Publishers
Published: 2006
Total Pages: 174
ISBN-13: 9781600210686
DOWNLOAD EBOOKEnzymatic processing of lipids and oils is becoming an important area of research. Hydrolytic enzymes, such as lipases and proteases are being sought after as the biocatalysts. This book focuses on the search and acquisition, isolation and purification and the characterisation of these enzymes
Author: Noor Zaliha Raja Abdul Rahman (Raja.)
Publisher: Nova Science Publishers
Published: 2013
Total Pages: 0
ISBN-13: 9781626186453
DOWNLOAD EBOOKThis book serves as a tool to expand the knowledge of researchers in the discipline of microbiology and biochemistry encompassing the fields of molecular biology, structural biology, and crystallography for the two very important industrial enzymes: proteases and lipases. In addition it covers the fermentation technology for the production of locally isolated microbial lipases and proteases. This book presents work done in our laboratory which extensively reviews two major types of lipases and proteases: thermostable and/or organic-solvent tolerant including a chapter on cold-active and organic solvent tolerant lipase and protease isolated from Antarctica. Detailed descriptions on the isolation methods, protein production, protein characterisation, and molecular cloning are discussed. Further works on structural biology of both proteases and lipases which include docking, molecular modification as well as protein crystallisation are also presented. For protein crystallographers, this book highlights a novel discovery of high temperature crystallisation of lipase.In addition, this book also offers a chapter on the cultivation strategy of lipase and protease in yeast expression systems and its bio-reactor studies. In short, this book is an all-in-one quick source of reference for researchers who want to have a quick grasp of understanding on many aspects of enzyme production, starting from the isolation and expression of the enzyme until crystallisation and structure elucidation of the protein.
Author: Uwe Theo Bornscheuer
Publisher: John Wiley & Sons
Published: 2006-05-12
Total Pages: 368
ISBN-13: 3527607129
DOWNLOAD EBOOKFrom reviews to the first edtion: "Bornscheuer and Kazlauskas have set out, and succeeded, in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and proteases) for organic chemists. This is quite simply the best book of its type and can be unreservedly recommended to organic chemists who have an interest in using hydrolytic enzymes in synthesis." (Nicholas J. Turner, University of Edinburgh) "The book is an indispensable source of information on the use of hydrolases in organic synthesis. The subject matter is very well set out, and the chapters are clearly written and presented from a critical viewpoint. Bornscheuer and Kazlauskas have succeeded admirably in describing the capabilities and limitations of the use of hydrolytic enzymes and in critically evaluating them. No library should be without the book." (Fritz Theil, WITEGA Angewandte Werkstoff-Forschung GmbH, Berlin) The second edition of this extremely successful and well-proven book presents recent developments in the use of hydrolases for organic synthesis, reflecting in particular the enormous progress made in enzyme discovery and optimization with a new chapter on "Protein Sources and Optimization of Biocatalyst Performance". The renowned authors survey the stereoselective reactions of hydrolases, especially lipases, esterases and proteases, giving researchers an overview of what has worked in the past so that they can judge how to solve their own synthetic problems. In total, the book contains over one thousand chemical structures, rounded off by some 1,800 invaluable references.
Author: Gerald Reed
Publisher: Elsevier
Published: 1966-01-01
Total Pages: 497
ISBN-13: 0323163076
DOWNLOAD EBOOKEnzymes in Food Processing describes the properties and practical applications of enzymes in food processing. This 20-chapter book includes applications such as the use of enzymes to tenderize meat, to produce dextrose, to clarify wine, to liquefy candy centers. The first part of this text is an introduction to the chemistry and kinetics of enzyme reactions. Chapters 2 to 5 describe the general nature of enzyme reactions, reaction rates, and the effect of pH and temperature, as well as the effect of inhibitors and activators on enzyme reactions. Chapters 6 to 9 examine specific enzymes, including the carbohydrases, proteases, lipases, and oxidoreductases, while Chapter 10 presents the methods of enzyme production. Considerable chapters are devoted to the application of enzymes in food processing. The chapters are arranged according to commodities, such as milling, baking, starch, dairy products, fruits, fruit products, wines, distilled alcoholic beverages, confectionary, and flavors. Chapter 19 and 20 includes a brief description of the closely related use of enzymes in feeds and as digestive aids, as well as the health and legal aspects of the use of enzymes. Food technologists, microbiologists, and enzyme chemists will find this book invaluable.
Author: Stephen Pandol
Publisher: Morgan & Claypool Publishers
Published: 2011
Total Pages: 66
ISBN-13: 1615041389
DOWNLOAD EBOOKThe secretions of the exocrine pancreas provide for digestion of a meal into components that are then available for processing and absorption by the intestinal epithelium. Without the exocrine pancreas, malabsorption and malnutrition result. This chapter describes the cellular participants responsible for the secretion of digestive enzymes and fluid that in combination provide a pancreatic secretion that accomplishes the digestive functions of the gland. Key cellular participants, the acinar cell and the duct cell, are responsible for digestive enzyme and fluid secretion, respectively, of the exocrine pancreas. This chapter describes the neurohumoral pathways that mediate the pancreatic response to a meal as well as details of the cellular mechanisms that are necessary for the organ responses, including protein synthesis and transport and ion transports, and the regulation of these responses by intracellular signaling systems. Examples of pancreatic diseases resulting from dysfunction in cellular mechanisms provide emphasis of the importance of the normal physiologic mechanisms.
Author: Jean-Michel Mérillon
Publisher:
Published:
Total Pages:
ISBN-13: 9783319194561
DOWNLOAD EBOOKAuthor: Seema Anil Belorkar
Publisher: Academic Press
Published: 2021-11-19
Total Pages: 268
ISBN-13: 0323914373
DOWNLOAD EBOOKProtocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. - Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards - Presents easy to read, reproducible protocols for researchers and students across academia and industry - Includes color diagrams that illustrate key concepts
Author: Julio Polaina
Publisher: Springer Science & Business Media
Published: 2007-05-16
Total Pages: 629
ISBN-13: 1402053770
DOWNLOAD EBOOKRecent developments in genetic engineering and protein chemistry are bringing ever more powerful means of analysis to bear on the study of enzyme structure. This volume reviews the most important types of industrial enzymes. In a balanced manner it covers three interrelated aspects of paramount importance for enzyme performance: three-dimensional protein structure, physicochemical and catalytic properties, and the range of both classical and novel applications.
Author: Wolfgang Aehle
Publisher: John Wiley & Sons
Published: 2008-01-08
Total Pages: 516
ISBN-13: 3527617108
DOWNLOAD EBOOKLeading experts from all over the world present an overview of the use of enzymes in industry for: - the production of bulk products, such as glucose, or fructose - food processing and food analysis - laundry and automatic dishwashing detergents - the textile, pulp and paper and animal feed industries - clinical diagnosis and therapy - genetic engineering. The book also covers identification methods of new enzymes and the optimization of known ones, as well as the regulatory aspects for their use in industrial applications. Up to date and wide in scope, this is a chance for non-specialists to acquaint themselves with this rapidly growing field. '...The quality...is so great that there is no hesitation in recommending it as ideal reading for any student requiring an introduction to enzymes. ...Enzymes in Industry - should command a place in any library, industrial or academic, where it will be frequently used.' The Genetic Engineer and Biotechnologist 'Enzymes in Industry' is an excellent introduction into the field of applied enzymology for the reader who is not familiar with the subject. ... offers a broad overview of the use of enzymes in industrial applications. It is up-to-date and remarkable easy to read, despite the fact that almost 50 different authors contributed. The scientist involved in enzyme work should have this book in his or her library. But it will also be of great value to the marketing expert interested in the present use of enzymes and their future in food and nonfood applications.' Angewandte Chemie 'This book should be available to all of those working with, or aspiring to work with, enzymes. In particular academics should use this volume as a source book to ensure that their 'new' projects will not 'reinvent the wheel'.' Journal of Chemical Technology and Biotechnology
Author: B.N. Johri
Publisher: Springer Science & Business Media
Published: 2013-04-17
Total Pages: 355
ISBN-13: 9401592063
DOWNLOAD EBOOKAll important aspects of thermophilic moulds such as systematics, ecology, physiology and biochemistry, production of extracellular and intracellular enzymes, their role in spoilage of stores products and solid and liquid waste management, and general and molecular genetics have been dealt with comprehensively by experts in this book which covers progress in the field over the last 30 years since the seminal book Thermophilic Fungi published by Cooney and Emerson in 1964. The experts have reviewed extensive literature on all aspects of thermophilic moulds in a very comprehensive manner. This book will be useful for graduates as well as post-graduate students of life sciences, mycology, microbiology and biotechnology, and as a reference book for researchers.
