Diagnostic Virology Protocols

Diagnostic Virology Protocols

Author: John R. Stephenson

Publisher: Springer Science & Business Media

Published: 1998-08-28

Total Pages: 388

ISBN-13: 9780896034792

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A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.


New Developments in Diagnostic Virology

New Developments in Diagnostic Virology

Author: P.A. Bachmann

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 332

ISBN-13: 3642689493

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The contributions to this book derived from the Seventh Munich Symposium on Microbiology on June 3 and 4, 1981, which was organized by the WHO Centre for Collection and Evaluation of Data on Comparative Virology at the Institute of Medical Microbiology, Infectious and Epidemic Diseases, University of Munich, Federal Repub lic of Germany. One of our principal purposes was to establish a forum at which the comparative aspects of questions of current interest in the field of medical virology could be discussed. In addition to the presentation of recent fmdings in microbiology, our overall aim was to crystallize trends and indicate new directions for future research activities. This book is a topical review of "New Horizons in Diagnostic Virology." Every one interested in virology is aware of the tremendous progress made in viral diagnostic techniques during recent years and the growing importance of viral diagnosis in human and veterinary medicine. There is yet another step that diagnostic virology has to take: the introduction on a routine basis of methods of molecular biology into the viral diagnostic laboratory. The application of monoclonal antibodies and techniques for the chemical and biological identification of proteins, carbohydrates, and enzymes are discussed, as is the introduction of techniques for the characterization of nucleic acids in viral diag nosis.


Guide to Clinical and Diagnostic Virology

Guide to Clinical and Diagnostic Virology

Author: Reeti Khare

Publisher: John Wiley & Sons

Published: 2020-08-06

Total Pages: 460

ISBN-13: 155581994X

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The explosion in clinical testing has been especially rapid in virology, where emerging viruses and growing numbers of viral infections are driving advances. The Guide to Clinical and Diagnostic Virology offers a digestible view of the breadth and depth of information related to clinical virology, providing a practical, working knowledge of the wide array of viruses that cause human disease. Introductory chapters cover the basics of clinical virology and laboratory diagnosis of infections, including virus structure, life cycle, transmission, taxonomy, specimen types and handling, and a comparison of assays used for detection. Detailed sections on important topics include Viral pathogens and their clinical presentations Diagnostic assays and techniques, including culture-based, immunological, and molecular Prevention and management of viral infections, with guidance on biosafety, vaccines, and antiviral therapies The regulatory environment for laboratory testing, including regulatory requirements and assay performance and interpretation Critical concepts are carefully curated and concisely summarized and presented with detailed illustrations that aid comprehension, along with important highlights and helpful hints. These features, plus question sections that reinforce significant ideas and key concepts, make this an invaluable text for anyone looking for an accessible route through clinical and diagnostic virology. Laboratory technologists, medical students, infectious disease and microbiology fellows, pathology residents, researchers, and everyone involved with viruses in the clinical setting will find the Guide to Clinical and Diagnostic Virology an excellent text as well as companion to clinical virology references.


Hsiung's Diagnostic Virology

Hsiung's Diagnostic Virology

Author: Gueh Djen Hsiung

Publisher: Yale University Press

Published: 1994-01-01

Total Pages: 420

ISBN-13: 9780300058451

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This generously illustrated book, the fourth edition of the highly acclaimed Diagnostic Virology, is a handbook for the recognition and characterization of virus-induced cellular changes seen by light microscopy, as well as virus morphology seen by electron microscopy. The authors cover all aspects of the subject, from specimen collection and laboratory safety to virus isolation and identification and cell culture. This edition includes a new chapter on the human retroviruses, especially relevant since the onset of the AIDS epidemic, and expands the chapters on the arboviruses and hepatitis viruses.


Clinical and Diagnostic Virology

Clinical and Diagnostic Virology

Author: Goura Kudesia

Publisher: Cambridge University Press

Published: 2009-02-19

Total Pages: 281

ISBN-13: 0521694671

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Practical text provides quick access to key diagnostic features of each virus encountered in clinical practice and their management.


Advanced Techniques in Diagnostic Microbiology

Advanced Techniques in Diagnostic Microbiology

Author: Yi-Wei Tang

Publisher: Springer

Published: 2018-11-09

Total Pages: 760

ISBN-13: 3319951114

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In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.


Recent Advances in Virus Diagnosis

Recent Advances in Virus Diagnosis

Author: M.S. McNulty

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 221

ISBN-13: 9400960395

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The traditional approach to diagnosis of virus infectians by isolation of the causative virus is usually both slow and expensive. More recently, the emphasis has been on the direct detection of viruses or viral antigens in clinical specimens. This can be done using established techniques such as immunofluorescence or electron microscopy, or by newly developed biochemical methods. The purpose of this meeting was to review these and other developments in the laboratory diagnosis of virus infections. We would like to thank all those who contributed to the success of this meeting. In particular, we are grateful to the CEC for financial sponsorship, to Professor C. Dow, Director of the Veterinary Research Laboratories, for help in organising the meeting, and to Miss B. Hamilton for her excellent typing of the manuscripts. M S McNulty J B McFerran VII CONTENTS Page Preface V VIRAL DIAGNOSIS BY TIMMUNOFLUORESCENCE P. S. Gardner APPLICATIONS OF IMMUNOFLUORESCENCE IN VETERINARY VIRAL DIAGNOSIS M. S. McNulty and G. M. Allan 15 TAGGING OF VIRAL ANTIGENS USING IMMUNOPEROXIDASE . AND IMMUNOOOLD TEGINlQUES R. Ducatelle, F. Castryck and J. Hoorens 27 DETECTION OF BVD VIRUS IN VlRAEMIC CATTLE BY AN INDIRECT IMMUNOPEROXIDASE TEGINIQUE A. Meyling 37 VIRAL DIAGNOSIS BY ELECTRON MICROSCOPY J. D. Almeida 47 CAPTURE ASSAYS FOR THE DETECTION OF VIRUS-SPECIFIC IgM ANTIBODY R. S. Tedder 60 DETECTION . AND IDENTIFICATION OF PAPILLOMAVIRUSES IN BENIGN . AND MALIGNANT 'TIlmJRS OF CATTLE M. S. Campo 72 DETECTION OF VIRAL NUCLEIC ACIDS IN CELL CULTURES .


Diagnostic Pathology of Infectious Disease E-Book

Diagnostic Pathology of Infectious Disease E-Book

Author: Richard L. Kradin

Publisher: Elsevier Health Sciences

Published: 2017-04-26

Total Pages: 715

ISBN-13: 0323508677

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Comprehensive and up to date, the Second Edition of Diagnostic Pathology: Infectious Disease, by Dr. Richard Kradin, is an invaluable tool for the accurate diagnosis of any infectious disease―from the common to the most challenging. The organ-based format makes it an especially useful tool for surgical pathologists' daily diagnostic and management issues. High-quality, full-color illustrations and differential diagnosis tables accompany each lesion, clearly depicting how to recognize the morphology of organisms and the spectrum of histological responses that they may cause. - Addresses the most difficult diagnostic issues that practicing or trainee surgical pathologists face when handling infectious disease tissue specimens. - Highlights morphological characteristics and landmarks of tissue samples for easy access to information necessary for signing out a specimen. - Emphasizes the host responses critical in differential diagnosis to serve as a second opinion when non-infectious diagnoses mimic and confound the diagnosis of infection. - Completely revised with the latest diagnostic support and hot topics in the field: - A new chapter on novel techniques in microbiology - A new chapter on eye infections - New coverage of immunohistochemical staining and other molecular diagnostic techniques - New discussions of human papillomavirus, a critical tool in predictive cancer screening - New information on infections in the immunocompromised host and related special considerations - Expert Consult eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, images, videos (including video updates), glossary, and references from the book on a variety of devices.


Veterinary Virology

Veterinary Virology

Author: Frank J. Fenner

Publisher: Academic Press

Published: 2014-06-28

Total Pages: 673

ISBN-13: 1483257819

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Veterinary Virology deals with basic biomedical virology and the clinical discipline of infectious diseases. The book discusses the principles of virology as effecting future developments in the search for preventive and management of infectious diseases in animals, whether singly or as a whole herd or flock. Part I explains the principles of animal virology including the structure, composition, classification, nomenclature, cultivation, and assay of viruses. This part also discusses viral genetics, replication, and evolution (including mutation and genetic engineering). The book also reviews the pathogenesis of viruses, host resistance and susceptibility, as well as the mechanisms of persistent infections and tumor induction. Part II deals with viruses found in domestic animals; this part also explains in detail the properties, replication methods, pathogenesis, immunity, diagnosis, and control of some common viruses. The book discusses some other families of viruses of which no members are yet known as to have caused serious or important diseases in animals. Veterinarians, immunologists, virologists, molecular researchers, students, and academicians in the discipline of virology and cellular biology, as well as livestock owners will find this book helpful.


Clinical Virology

Clinical Virology

Author: Douglas D. Richman

Publisher: John Wiley & Sons

Published: 2020-07-10

Total Pages: 1473

ISBN-13: 1555819435

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The essential reference of clinical virology Virology is one of the most dynamic and rapidly changing fields of clinical medicine. For example, sequencing techniques from human specimens have identified numerous new members of several virus families, including new polyomaviruses, orthomyxoviruses, and bunyaviruses. Clinical Virology, Fourth Edition, has been extensively revised and updated to incorporate the latest developments and relevant research. Chapters written by internationally recognized experts cover novel viruses, pathogenesis, epidemiology, diagnosis, treatment, and prevention, organized into two major sections: Section 1 provides information regarding broad topics in virology, including immune responses, vaccinology, laboratory diagnosis, principles of antiviral therapy, and detailed considerations of important organ system manifestations and syndromes caused by viral infections. Section 2 provides overviews of specific etiologic agents and discusses their biology, epidemiology, pathogenesis of disease causation, clinical manifestations, laboratory diagnosis, and management. Clinical Virology provides the critical information scientists and health care professionals require about all aspects of this rapidly evolving field.