Edited by the experts and pioneers in the field, this is the first monograph to cover the topic, containing the must-have information hitherto only scattered among journals. Clearly divided into sections on preparation, characterization and modeling, and applications, this is essential reading for chemists, chromatographers, analytical chemists, biochemists and biologists.
During the past decade, monolithic materials in the shape of discs, stacked layers, rolled sheets, sponges, irregular chunks, tubes, and cylinders have all been successfully demonstrated. These formats were prepared from a wide variety of materials including natural polymers such as cellulose, synthetic polymers that involved porous styrene-, methacrylate-, and acrylamide-based polymers, and inorganic materials, mainly silica. Each approach is interesting from the point of view of both preparation and application.Although the current papers and patents concerned with monolithic separation media are quite numerous, the information is scattered throughout a vast number of journals. This book therefore fills the gap in the market for a comprehensive reference book on this subject.Monolithic materials concerns all of the current formats of monolithic materials and provides an integrated view of this novel format of separation media. Since the flow pattern in monolithic devices is different from that in packed beds, the hydrodynamics of the system and mass transport differ considerably from those derived for packed columns. Therefore, this book presents contributions concerned with both flow and mass transfer in the monolithic materials. A significant proportion of the book is devoted to the applications of monolithic materials. It also provides the reader with valuable information about the sources of the specific materials, their properties, and potential applications.·Monolithic materials are currently very popular within several scientific areas such as chromatography, optics, catalysis, diagnostics, genomics, proteomics, and microfluidics.·Provides valuable information about the sources of the specific materials, their properties, and potential applications.·Chapters written by leading experts in the area.
High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the
An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include: * Comprehensive overview of state-of-the-art HPLC column technology * Explanation of the underlying principles of HPLC columns * Methods for selecting columns * Practical advice on using and applying columns, including examples * Section by M. Zoubair El Fallah on methods development * Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed * Troubleshooting section HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses.
This book is a contemporary review of selected subjects in liquid chromatography, especially of the technical development, rather than the applications. The subjects are focused in the biomedical and environmental fields. This is also a troubleshooting record. Complex analytical problems such as sensitivity (sensitive detection by chemiluminescence, coulometric detection, laser based detection, necessity of degassing the system for sensitive detection), difficulty (free radical detection by Electron Spin Resonance, Polarimeter for chiral recognition) and reproducibility (packings for chiral separation and stable bonded silica gels) are solved. Theoretically and environmentally important miniaturizations are described. Individual chapters written by specialists provide information beyond what can be found in general textbooks of liquid chromatography.
A comprehensive guide to smart materials and how they are used in sample preparation, analytical processes, and applications This comprehensive, two-volume handbook provides detailed information on the present state of new materials tailored for selective sample preparation and the legal frame and environmental side effects of the use of smart materials for sample preparation in analytical chemistry, as well as their use in the analytical processes and applications. It covers both methodological and applied analytical aspects, relating to the development and application of new materials for solid-phase extraction (SPE) and solid-phase microextraction (SPME), their use in the different steps and techniques of the analytical process, and their application in specific fields such as water, food, air, pharmaceuticals, clinical sciences and forensics. Every chapter in Handbook of Smart Materials in Analytical Chemistry is written by experts in the field to provide a comprehensive picture of the present state of this key area of analytical sciences and to summarize current applications and research literature in a critical way. Volume 1 covers New Materials for Sample Preparation and Analysis. Volume 2 handles Analytical Processes and Applications. Focuses on the development and applications of smart materials in analytical chemistry Covers both, methodological and applied analytical aspects, for the development of new materials and their use in the different steps and techniques of the analytical process and their application in specific fields Features applications in key areas including water, air, environment, pharma, food, forensic, and clinical Presents the available tools for the use of new materials suitable to aid recognition process to the sample preparation and analysis A key resource for analytical chemists, applied laboratories, and instrument companies Handbook of Smart Materials in Analytical Chemistry, 2V Set is an excellent reference book for specialists and advanced students in the areas of analytical chemistry, including both research and application environments.
Endlich ein Forschungsleitfaden für Wissenschaftler des Fachgebiets, die neue Methoden entwickeln oder einsetzen. Dieses Handbuch umfasst fünf thematische Bände und bietet damit einen umfassenden Überblick über das Fachgebiet. Erläutert werden Grundlagen, die Methodenentwicklung und hochkarätige Anwendungen für alle wichtigen Analyseverfahren, darunter chromatische Verfahren, Techniken in den Bereichen Elektromigration und Membranen. Dieses Referenzwerk umfasst ein breites Spektrum und legt den Schwerpunkt auf Entwicklungen für die Zukunft. Damit ist es ein Muss für Forscher und eine wertvolle Wissensquelle für Studenten im Hauptstudium und Studienabsolventen.
Revised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis Following in the footsteps of the previously published Handbook of LC-MS Bioanalysis, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography–Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare. While advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include: Current basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes) Sample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor) Crucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates) Sample Preparation in LC-MS Bioanalysis will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring.
