Aqueous Two-Phase Systems
Aqueous Two-Phase Systems

Author: Rajni Hatti-Kaul

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 442

ISBN-13: 1592590284

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A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Partitioning In Aqueous Two – Phase System
Partitioning In Aqueous Two – Phase System

Author: Harry Walter

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 731

ISBN-13: 0323142915

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Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Biothermodynamics
Biothermodynamics

Author: Urs von Stockar

Publisher: CRC Press

Published: 2013-05-30

Total Pages: 628

ISBN-13: 1466582170

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This book covers the fundamentals of the rapidly growing field of biothermodynamics, showing how thermodynamics can best be applied to applications and processes in biochemical engineering. It describes the rigorous application of thermodynamics in biochemical engineering to rationalize bioprocess development and obviate a substantial fraction of t

Aqueous Biphasic Separations
Aqueous Biphasic Separations

Author: Robin D. Rogers

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 191

ISBN-13: 1461519535

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Proceedings of an American Chemical Society Symposium held in San Diego, California, March 13-14, 1994

Bioseparations Science and Engineering
Bioseparations Science and Engineering

Author: Roger G. Harrison

Publisher: Oxford University Press

Published: 2015-01-27

Total Pages: 577

ISBN-13: 0190213736

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Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field of bioseparations. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro Designer® is used to analyze and evaluate the production of three important biological products. New to this second edition are updated discussions of moment analysis, computer simulation, membrane chromatography, and evaporation, among others, as well as revised problem sets. Unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals working in or studying bioseparations, and is the premier text in the field.

The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering
The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering

Author: John Paul Frampton

Publisher: Frontiers Media SA

Published: 2020-01-13

Total Pages: 117

ISBN-13: 288963356X

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The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.