E. coli Gene Expression Protocols
E. coli Gene Expression Protocols

Author: Peter E. Vaillancourt

Publisher: Springer Science & Business Media

Published: 2008-02-02

Total Pages: 347

ISBN-13: 1592593011

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Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.

Molecular Cytology V1
Molecular Cytology V1

Author: Jean Brachet

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 439

ISBN-13: 0323150349

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Molecular Cytology presents an integrated version about the morphology and biochemistry of the cell. This two-volume book focuses on the dynamic aspects of cytology and on the nucleocytoplasmic interactions in unicellular organisms and eggs. The first chapter covers the history of cell, cytology, and nucleic acids, as well as the uniformity and diversity in cell. The book then discusses various methods used in cell biology, including optical, cytochemical, biological, biochemical, and biophysical techniques. It also examines the activities of cytoplasm and nucleus during interphase. The final chapter describes various phases of the cell cycle, the structure of metaphase chromosomes, the molecular organization of the mitotic apparatus, and the cytokinesis, with emphasis on the main mitotic abnormalities. With the aim of linking the morphology and biochemistry of the cell, this book is intended for advanced students, research workers, biochemists, and cytologists who wish to broaden their knowledge in cell.

E. Coli Plasmid Vectors
E. Coli Plasmid Vectors

Author: Nicola Casali

Publisher: Taylor & Francis US

Published: 2003-07-03

Total Pages: 336

ISBN-13: 9781588291516

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This concise reference focuses on the manipulation of plasmids in Escherichia coli.

E. coli Gene Expression Protocols
E. coli Gene Expression Protocols

Author: Peter E. Vaillancourt

Publisher: Humana Press

Published: 2002-10-22

Total Pages: 347

ISBN-13: 9781588290083

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Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.