Microbial Gene Techniques, Part B
Microbial Gene Techniques, Part B

Author:

Publisher: Academic Press

Published: 1995-08-25

Total Pages: 507

ISBN-13: 0080536395

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Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages.* * Methods presented for ease of use and ready adaptation to new systems.* Detailed protocols included for:* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.

Safety of Genetically Engineered Foods
Safety of Genetically Engineered Foods

Author: National Research Council

Publisher: National Academies Press

Published: 2004-07-08

Total Pages: 254

ISBN-13: 0309166152

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Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Viral Gene Techniques
Viral Gene Techniques

Author:

Publisher: Elsevier

Published: 1995-11-14

Total Pages: 436

ISBN-13: 0080536409

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Viral Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The volume is concerned with methods for the analysis of viral genes and chromosomes: DNA viruses and RNA viruses including HIV are discussed.* Methods presented for ease of use and reasdy adaptation to new systems* Detailed experimental protocols included for:* Viral vectors - construction and use of DNA virus vectors (adenovirus, adeno-associated virus, vaccinia virus, Epstein - Barr virus)* DNA viruses - virus/host interactions, viral chromosomes , transcription regulation (viruses discussed include herpes simplex, hepatitis B, SV40, JC, Epstein-Barr, adenovirus)* Human Immunodeficiency Virus / retroviruses -quantitation of HIV-1 virus stock and RNA, retrovirus reverse transcription / integration, retrovirus-mediated cell fusion, use as cell lineage markers* RNA viruses - RNA virus assembly, analysis of RNA genomes, assays for RNA-binding proteins (viruses discussed include poliovirus, influenza virus, hepatitis delta virus)

Human Molecular Genetics
Human Molecular Genetics

Author:

Publisher: Academic Press

Published: 1996-05-23

Total Pages: 521

ISBN-13: 0080536417

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Human Molecular Genetics is a practical guide to the applications of molecular biology and genetics techniques to human cells. A wide range of experimental procedures for investigating human genes and genomes are presented.* * Mutation Detection in Human Genes - chemical mismatch cleavage, DNA mini-sequencing, SSCP method, RT-PCR, electrophoretic mobility shift assay (EMSA), protein truncation test, chromosome deletion analysis.* Gene Mapping, Cloning, Sequencing - gene linkage determination, large-capacity cloning system, cDNA isolation, differential display method, primer-based DNA sequencing.* Transcription: Promoters, Transcription Factors, mRNA, - promotor mutation analysis, transcription factor identification, mRNA-protein interaction characterization.* RNA Editing, Ribozymes, Antisense RNA-mammalian RNA editing assays, ribozymes as genetic tools, antisense RNA technology.* Genome Recombination, Amplification - recombination assays for mammalian cells, gene amplification measurement.* Receptors, Signal Transduction - intra-cellular receptor characterization, analysis of signal transduction genes.* The Mouse as a Model System for Human Molecular Genetics - mouse genome methods (mouse crosses, somatic cell hybrids, YACs), mouse model for cardiovascular disease.

Molecular Biology Techniques
Molecular Biology Techniques

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Applications of Chimeric Genes and Hybrid Proteins, Part B: Cell Biology and Physiology
Applications of Chimeric Genes and Hybrid Proteins, Part B: Cell Biology and Physiology

Author:

Publisher: Elsevier

Published: 2000-10-17

Total Pages: 710

ISBN-13: 0080496822

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Chemical Microbiology Part B
Chemical Microbiology Part B

Author:

Publisher: Academic Press

Published: 2022-04-02

Total Pages: 394

ISBN-13: 0323952666

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Chemical Microbiology, Part B, Volume 665, the latest release in the Methods of Enzymology series, highlights new advances in the field, including comprehensive chapters on the Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies, Metabolomic approaches for enzyme function and pathway discovery in bacteria, Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance, Development and application of highly sensitive labeling reagents for amino acids, Bacterial Cell Wall Modification with a Glycolipid Substrate, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Updated release includes the latest information on chemical microbiology

Molecular Microbiology Techniques
Molecular Microbiology Techniques

Author: Kenneth W. Adolph

Publisher:

Published: 1994

Total Pages: 428

ISBN-13:

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The new series METHODS IN MOLECULAR GENETICS provides practical experimental procedures for use in the laboratory. Because the introduction of molecular genetic techniques has revolutionized biological research, a wide range of methods is covered. This volume of METHODS IN MOLECULAR GENETICS presents up-to-date practical molecular biology and genetics techniques of the analysis of microbial genes and chromosomes, including those od eukaryotic andf prokaryotic cells and plasmids. * * Methods presented for easy use and ready adaptation to new systems * Detailed experimental protocols included for: * * Eukaryotic microbes - yeast (mutants, transposons, viruses), parasites (gene identificationa dn regulatino), slime mold (transformation) * * Bacterial DNA and Chromosmes - codon usage, quantitation of RNA transcription, challenge phage, cell division, motility and chemotaxis * * Bacterial Gene Analysis - gel shift assay, DNase 1 footprinting, gene fusions, membrane protein genes, oxidative stress genes * * Plasmids - assays for DNA and DNA-binding proteins

Natural Product Biosynthesis by Microorganisms and Plants Part B
Natural Product Biosynthesis by Microorganisms and Plants Part B

Author:

Publisher: Academic Press

Published: 2012-10-01

Total Pages: 463

ISBN-13: 012394628X

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants. - This new volume continues the legacy of this premier serial - Contains quality chapters authored by leaders in the field - The second of 3 volumes it has chapters on such topics as biological chlorination, bromination and iodination, and phylogenetic approaches to natural product structure prediction