Manual of Embryo Culture in Human Assisted Reproduction

Manual of Embryo Culture in Human Assisted Reproduction

Author: Kersti Lundin

Publisher: Cambridge University Press

Published: 2021-05-06

Total Pages: 125

ISBN-13: 110887536X

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Whilst assisted reproduction techniques (ART) have become increasingly successful and largely standardized, there is still only a partial understanding of what constitutes a 'true' embryo environment. Replicating the varying physiological conditions of the in-vivo environment that the embryo travels through in the in-vitro culture is still a major challenge in ART. This practical volume details how to organize and operate an IVF laboratory in order to mimic these conditions for successful embryo culture. Environments and equipment that are essential for running safe and efficient facilities such as maintaining good air quality and hygiene protocols, and utilizing an effective layout are covered in detail. Other chapters discuss the different consumables needed, optimal handling techniques and parameter monitoring systems, as well as recent advances in the area including artificial intelligence and automation. This is an indispensable guide to understanding the background science of culturing embryos, crucial to successful outcomes in ART.


Manual on Assisted Reproduction

Manual on Assisted Reproduction

Author: T. Rabe

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 668

ISBN-13: 3642583415

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Our knowledge of reproductive medicine has expanded rapidly since the birth of Louise Brown, the first baby to be conceived by in vitro fertilization, which was performed by Professors Patrick Steptoe and Bob Edwards in Oldham, Eng land, in 1978. Hardly a year goes by without the development of a new or a modification of an existing method of assisted reproduction. Within a relatively short period, in vitro fertilization has been introduced into the treatment of female infertility. Intracytoplasmic sperm injection has also created new opportunities for the treatment of male infertility. The first edition of this book was published in 1996. In the second edition most of the chapters have been updated and additional interest is focused on intracytoplasmic sperm injection (ICSI) in view of the risk of malformations in newborns. This manual addresses the techniques of assisted reproduction that are avail able today. Competent authors from various centers present, in a concise way, their tried-and-tested procedures, so that the latter can be readily implemented. Due to different legal regulations, the scope of assisted reproduction is much more limited in Germany than in many other countries. For example, whereas only three embryos may be created and transferred in Germany, such restric tions do not exist in several other European countries and the United States. Furthermore, heterologous fertilization, oocyte donation, and surrogate mother hood are banned in Germany.


Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction

Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction

Author: Gianpiero D. Palermo

Publisher: Cambridge University Press

Published: 2021-11-25

Total Pages: 175

ISBN-13: 1108743838

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"Not everything in medical science has a clear beginning. The first realization of infertility and putative remedies remain shrouded in contextual history, but likely goes back to the dawn of our species, well before there was a written record. Childlessness was, and is still, considered a burden in some communities"--


Vitrification in Assisted Reproduction

Vitrification in Assisted Reproduction

Author: Michael Tucker

Publisher: CRC Press

Published: 2007-11-01

Total Pages: 322

ISBN-13: 0203090020

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Cryopreservation methods have become well established as an increasingly important therapeutic strategy in assisted reproduction. Vitrification in Assisted Reproduction: A User's Manual and Trouble-shooting Guide addresses this cryopreservation technique from an academic and technical viewpoint, with specific reference to assisted reproduction in h


A Textbook of In Vitro Fertilization and Assisted Reproduction

A Textbook of In Vitro Fertilization and Assisted Reproduction

Author: Peter R. Brinsden

Publisher: CRC Press

Published: 1999-04-15

Total Pages: 780

ISBN-13: 9781850700005

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This is an enlarged, updated, color-illustrated new edition of the definitive clinical reference on in vitro fertilization and assisted reproduction. It contains 37 chapters by top-ranked specialists from around the world covering every aspect of investigation and therapeutic options as taught and practiced at the world-famous Bourn Hall Clinic. Large format, double-column pages. Includes bibliographic references, procedures, protocols and information sheets, and index.


Manual of Equine Reproduction

Manual of Equine Reproduction

Author: Steven P. Brinsko

Publisher: Elsevier Health Sciences

Published: 2010-05-19

Total Pages: 337

ISBN-13: 0323065139

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Now in full color, Manual of Equine Reproduction, 3rd Edition provides a comprehensive look at the reproductive management of horses, including management of stallions, pregnant mares, and neonatal foals. Expert authors use a concise, practical approach in discussing improved therapies and treatments in equine breeding. You'll enhance your skills and knowledge with this book's detailed coverage of techniques used in reproductive examination, breeding procedures, pregnancy diagnosis, foaling, and reproductive tract surgery. - A clinical emphasis includes a step-by-step format of possible scenarios from conception to breeding management. - Practical information includes topics such as breeding with transported cooled or frozen semen, and caring for the broodmare and newborn foal. - The organization of material corresponds to the course of study in veterinary school, so you can find topics easily. - Chapter objectives and study questions at the beginning of each chapter guide you through the material and provide clear learning goals. - Evaluation of Breeding Records chapter covers the importance of breeding records, and how to use them to evaluate stallion performance and optimize fertility. - References are listed at the end of each chapter for further research and study. - Full-color photographs and illustrations clearly depict procedures, and all drawings have been redrawn and improved. - NEW Assisted Reproductive Technology chapter goes beyond embryo transfer. - Updated content includes the latest advances in therapies and treatments. - New content is added to two chapters, Reproductive Physiology of the Nonpregnant Mare and Manipulation of Estrus in the Mare. - Thorough coverage of every aspect of equine reproduction provides a strong foundation for success in veterinary practice, including a discussion of the use of GnRH-analog deslorelin (Ovuplant) to hasten ovulation; aseptic technique for endometrial biopsy; use of transabdominal ultrasonography, especially in early pregnancy; determination of fetal gender by transrectal ultrasonography; aspiration testicular biopsy using a spring-loaded biopsy instrument; and procedure for surgical embryo transfer.


Principles and Practice of Assisted Reproductive Technology

Principles and Practice of Assisted Reproductive Technology

Author: Kamini A Rao

Publisher: Jaypee Brothers Medical Publishers

Published: 2023-01-18

Total Pages: 1739

ISBN-13: 9356960372

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VOLUME 1: INFERTILITY SECTION 1: ANATOMY AND PHYSIOLOGY 1. Anatomy of the Reproductive System 2. Regulation and Physiology of Menstrual Cycle 3. Oogenesis and Folliculogenesis 4. Spermatogenesis 5. Fertilization and Embryogenesis 6. Implantation 7. Embryo Endometrial Crosstalk and Endometrial Receptivity SECTION 2: REPRODUCTIVE ENDOCRINOLOGY 8. Synthesis and Metabolism of Steroid Hormones 9. Puberty and Aberrations 10. Amenorrhea 11. Endocrine Disorders Affecting Reproduction 12. Hirsutism 13. Luteal Phase Defect 14. Anovulation 15. Declining Fertility SECTION 3: COMBINED TOPICS 16. Evaluation of Infertility 17. Immunology and Infertility 18. Cytogenetics and Subfertility 19. Obesity and Infertility 20. Unexplained Infertility 21. Fertility Preservation 22. Counseling in Infertility 23. Assisted Reproductive Technology in Patients with Chronic Medical Disorders SECTION 4: MALE INFERTILITY 24. Etiopathogenesis of Male Infertility 25. Clinical and Endocrinological Evaluation of Infertile Male 26. Sexual Dysfunction in Male Infertility 27. Ultrasound in Male Infertility 28. Medical Management of Male Infertility 29. Azoospermia: Evaluation and Management 30. Varicocele and Infertility 31. Spinal Cord Injuries and Male Infertility 32. Algorithms for Genetic Evaluation of Infertile Males SECTION 5: FEMALE FACTOR INFERTILITY 33. Uterine Factors in Infertility 34. Tubal Factors in Infertility 35. Infections and Infertility 36. Tuberculosis and Infertility 37. Sonoendocrinology and Cycle Monitoring Assisted Reproduction Technology 38. Transvaginal Ultrasound and Doppler in Infertility 39. Polycystic Ovary Syndrome 40. Assessment of Ovarian Reserve 41. Endometriosis 42. Endoscopy in Infertility 43. Reconstructive Surgeries Enhancing Fertility SECTION 6: INTRAUTERINE INSEMINATION 44. Intrauterine Insemination 45. Optimizing Success in Intrauterine Insemination SECTION 7: OVARIAN STIMULATION 46. Drugs for Ovarian Stimulation 47. Ovulation Induction and Ovarian Stimulation Protocols 48. Role of Adjuvants in Ovarian Stimulation 49. Gonadotropinreleasing Hormone Analogs 50. Monitoring of Ovarian Stimulation 51. Ovulation Trigger 52. Individualized Controlled Ovarian Stimulation 53. In Vitro Fertilization Lite 54. Role of Luteinizing Hormone in Ovarian Stimulation 55. Anesthesia in Assisted Reproductive Techniques 56. Oocyte Retrieval. 57. Embryo Transfer 58. Troubleshooting in Assisted Reproductive Technology 59. Luteal Phase Support SECTION 8: DILEMMA IN ART 60. Poor Responder 61. Recurrent Implantation Failure 62. Empty Follicle Syndrome 63. Role of Aneuploidy Screening in Preimplantation Embryos 64. Preimplantation Genetic Testing of Embryos 65. Epigenetics and Assisted Reproductive Technology SECTION 9: COMPLICATIONS IN ART 66. Ovarian Hyperstimulation Syndrome 67. Ectopic Pregnancy 68. Multipleorder Births SECTION 10: THIRD PARTY REPRODUCTION 69. Oocyte and Sperm Donation 70. Surrogacy in Assisted Reproductive Technology 71. Assisted Reproductive Technology Guidelines 72. Adoption 73. LGBTQ and Fertility 74. Transgender Population and Fertility SECTION 11: OUTCOME FOLLOWING ASSISTED REPRODUCTIVE TECHNIQUE 75. Maternal and Fetal Outcomes Following Assisted Reproductive Technique 76. Early Pregnancy Scan 77. Recurrent Pregnancy Loss: From Diagnostic Dilemmas to Clinical Decisions SECTION 12: RECENT ADVANCES 78. Bioengineered Human Endometrium In Vitro. 79. Recent Trends in A...


The Boston IVF Handbook of Infertility

The Boston IVF Handbook of Infertility

Author: Steven Bayer

Publisher: CRC Press

Published: 2017-09-01

Total Pages: 260

ISBN-13: 1351241486

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Based on the gold standard procedures and protocols developed at Boston IVF, this new edition of a bestselling text continues to provide a structured approach to treating the infertile couple that can be of benefit to the gynecologist, reproductive endocrinologist, and reproductive medicine nurse alike. Both clinical and laboratory techniques are included, with material on preconception care. New to this edition are chapters on fertility care for the LGBT community, endometriosis, elective egg freezing, and effective nursing.