Manipulating the Mouse Embryo
Manipulating the Mouse Embryo

Author: Brigid Hogan

Publisher: Cold Spring Harbor Laboratory Press

Published: 1986

Total Pages: 352

ISBN-13:

DOWNLOAD EBOOK

Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.

Manipulating the Mouse Embryo
Manipulating the Mouse Embryo

Author: Richard Behringer

Publisher:

Published: 2014

Total Pages: 0

ISBN-13: 9781936113019

DOWNLOAD EBOOK

Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.

Safety of Genetically Engineered Foods
Safety of Genetically Engineered Foods

Author: National Research Council

Publisher: National Academies Press

Published: 2004-07-08

Total Pages: 254

ISBN-13: 0309166152

DOWNLOAD EBOOK

Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Scientific Frontiers in Developmental Toxicology and Risk Assessment
Scientific Frontiers in Developmental Toxicology and Risk Assessment

Author: National Research Council

Publisher: National Academies Press

Published: 2000-12-21

Total Pages: 348

ISBN-13: 0309070864

DOWNLOAD EBOOK

Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.

New Principles in Developmental Processes
New Principles in Developmental Processes

Author: Hisato Kondoh

Publisher: Springer

Published: 2014-07-08

Total Pages: 316

ISBN-13: 4431546340

DOWNLOAD EBOOK

During the last decade, modern technologies have made a revolutionary change in developmental biology. The molecular and cellular processes in live embryos can now be visualized thanks to technologies using fluorescent proteins. The whole genome information of a wide range of animal species has now become available, confirming the common principles that operate in every species. These and other advances in our understanding of the developmental processes during embryogenesis and tissue regeneration have put forward new principles. Those new principles will also be important in the stem cell biology, branched from developmental biology, in order to generate a particular tissue by manipulating stem cells. This book is planned to introduce these new principles to readers who are working in developmental biology and/or stem cell biology fields, with an emphasis on genetic and cellular processes.

Maternal Control of Development in Vertebrates
Maternal Control of Development in Vertebrates

Author: Florence Louise Marlow

Publisher: Morgan & Claypool Publishers

Published: 2010

Total Pages: 221

ISBN-13: 161504051X

DOWNLOAD EBOOK

Eggs of all animals contain mRNAs and proteins that are supplied to or deposited in the egg as it develops during oogenesis. These maternal gene products regulate all aspects of oocyte development, and an embryo fully relies on these maternal gene products for all aspects of its early development, including fertilization, transitions between meiotic and mitotic cell cycles, and activation of its own genome. Given the diverse processes required to produce a developmentally competent egg and embryo, it is not surprising that maternal gene products are not only essential for normal embryonic development but also for fertility. This review provides an overview of fundamental aspects of oocyte and early embryonic development and the interference and genetic approaches that have provided access to maternally regulated aspects of vertebrate development. Some of the pathways and molecules highlighted in this review, in particular, Bmps, Wnts, small GTPases, cytoskeletal components, and cell cycle regulators, are well known and are essential regulators of multiple aspects of animal development, including oogenesis, early embryogenesis, organogenesis, and reproductive fitness of the adult animal. Specific examples of developmental processes under maternal control and the essential proteins will be explored in each chapter, and where known conserved aspects or divergent roles for these maternal regulators of early vertebrate development will be discussed throughout this review. Table of Contents: Introduction / Oogenesis: From Germline Stem Cells to Germline Cysts / Oocyte Polarity and the Embryonic Axes: The Balbiani Body, an Ancient Oocyte Asymmetry / Preparing Developmentally Competent Eggs / Egg Activation / Blocking Polyspermy / Cleavage/ Mitosis: Going Multicellular / Maternal-Zygotic Transition / Reprogramming: Epigenetic Modifications and Zygotic Genome Activation / Dorsal-Ventral Axis Formation before Zygotic Genome Activation in Zebrafish and Frogs / Maternal TGF-β and the Dorsal-Ventral Embryonic Axis / Maternal Control After Zygotic Genome Activation / Compensation by Stable Maternal Proteins / Maternal Contributions to Germline Establishment or Maintenance / Perspective / Acknowledgments / References