Gene Expression Analysis
Gene Expression Analysis

Author: Nalini Raghavachari

Publisher: Humana

Published: 2018-05-17

Total Pages: 0

ISBN-13: 9781493978335

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This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gene Expression Analysis: Methods and Protocols aims provide useful information to researchers worldwide.

Recombinant Gene Expression
Recombinant Gene Expression

Author: Paulina Balbas

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 505

ISBN-13: 1592597742

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Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.

Journal Spilling
Journal Spilling

Author: Trout. Diana

Publisher: North Light Books

Published: 2009-10-09

Total Pages: 128

ISBN-13: 9781600613197

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Go ahead—make a mess! There are no lines to stay inside of here. You're free to quiet your inner critic and spill color (as well as your thoughts) all over the page. Author Diana Trout offers a double-dose of encouragement for you to try out new techniques, to ask yourself new questions and to see how safe of a place your private journal can truly be. Whether you've been carrying around an art journal for years, or have been waiting for just the right push, in the pages of Journal Spilling you will learn new ways with mixed media as well as new insights about yourself. Step-by-step instruction will make the process easy and you'll explore such techniques as: Using salt, alcohol and wax paper as resists for watercolor "Spilling" color over your page with the help of watercolor crayons Creating unique lines and shapes with a fun ink-blowing technique Making secret pockets and flaps for storing wishes and private reflections Carving and stamping with hand-carved stamps, making your pages even more personal Getting out of a writing rut with prompts and inspiring exercises and much more Find out just how fulfilling using creative expression in your personal journal can be and let Journal Spilling be the friend to offer you support for each page you create.

Recoding: Expansion of Decoding Rules Enriches Gene Expression
Recoding: Expansion of Decoding Rules Enriches Gene Expression

Author: John F. Atkins

Publisher: Springer Science & Business Media

Published: 2010-03-10

Total Pages: 473

ISBN-13: 0387893822

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The literature on recoding is scattered, so this superb book ?lls a need by prov- ing up-to-date, comprehensive, authoritative reviews of the many kinds of recoding phenomena. Between 1961 and 1966 my colleagues and I deciphered the genetic code in Escherichia coli and showed that the genetic code is the same in E. coli, Xenopus laevis, and guinea pig tissues. These results showed that the code has been c- served during evolution and strongly suggested that the code appeared very early during biological evolution, that all forms of life on earth descended from a c- mon ancestor, and thus that all forms of life on this planet are related to one another. The problem of biological time was solved by encoding information in DNA and retrieving the information for each new generation, for it is easier to make a new organism than it is to repair an aging, malfunctioning one. Subsequently, small modi?cations of the standard genetic code were found in certain organisms and in mitochondria. Mitochondrial DNA only encodes about 10–13 proteins, so some modi?cations of the genetic code are tolerated that pr- ably would be lethal if applied to the thousands of kinds of proteins encoded by genomic DNA.

Methods of Microarray Data Analysis IV
Methods of Microarray Data Analysis IV

Author: Jennifer S. Shoemaker

Publisher: Springer Science & Business Media

Published: 2006-01-16

Total Pages: 266

ISBN-13: 0387230777

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As studies using microarray technology have evolved, so have the data analysis methods used to analyze these experiments. The CAMDA conference plays a role in this evolving field by providing a forum in which investors can analyze the same data sets using different methods. Methods of Microarray Data Analysis IV is the fourth book in this series, and focuses on the important issue of associating array data with a survival endpoint. Previous books in this series focused on classification (Volume I), pattern recognition (Volume II), and quality control issues (Volume III). In this volume, four lung cancer data sets are the focus of analysis. We highlight three tutorial papers, including one to assist with a basic understanding of lung cancer, a review of survival analysis in the gene expression literature, and a paper on replication. In addition, 14 papers presented at the conference are included. This book is an excellent reference for academic and industrial researchers who want to keep abreast of the state of the art of microarray data analysis. Jennifer Shoemaker is a faculty member in the Department of Biostatistics and Bioinformatics and the Director of the Bioinformatics Unit for the Cancer and Leukemia Group B Statistical Center, Duke University Medical Center. Simon Lin is a faculty member in the Department of Biostatistics and Bioinformatics and the Manager of the Duke Bioinformatics Shared Resource, Duke University Medical Center.

Regulation of Gene Expression
Regulation of Gene Expression

Author: Gary H. Perdew

Publisher: Springer Science & Business Media

Published: 2008-08-17

Total Pages: 334

ISBN-13: 1597452289

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The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

The Social Nature of Emotion Expression
The Social Nature of Emotion Expression

Author: Ursula Hess

Publisher: Springer Nature

Published: 2019-12-12

Total Pages: 254

ISBN-13: 3030329682

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This book provides an overview of theoretical thinking about the communicative scope of emotional expressions as well as an overview of the state of the art research in emotional psychology. For many years, research in emotional psychology has been primarily concerned with the labeling of emotion expressions and the link between emotion expressions and the expresser’s internal state. Following recent trends in research devoting specific attention to the social signal value of emotions, contributors emphasize the nature of emotion expressions as information about the person and the situation, including the social norms and standards relevant to the situation. Focusing on the role of emotion expressions as communicative acts, this timely book seeks to advance a line of theoretical thinking that goes beyond the view of emotion expressions as symptoms of an intrapersonal phenomenon to focus on their interpersonal function. The Social Nature of Emotion Expression will be of interest to researchers in emotional psychology, as well as specialists in nonverbal behavior, communication, linguistics, ethology and ethnography.

Gene Expression Programming
Gene Expression Programming

Author: Candida Ferreira

Publisher: Springer

Published: 2006-08-29

Total Pages: 493

ISBN-13: 3540328491

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This book describes the basic ideas of gene expression programming (GEP) and numerous modifications to this powerful new algorithm. It provides all the implementation details of GEP so that anyone with elementary programming skills will be able to implement it themselves. The book includes a self-contained introduction to this new exciting field of computational intelligence. This second edition has been revised and extended with five new chapters.

Epigenetic Mechanisms of Gene Regulation
Epigenetic Mechanisms of Gene Regulation

Author: Vincenzo E. A. Russo

Publisher:

Published: 1996

Total Pages: 716

ISBN-13:

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Many inheritable changes in gene function are not explained by changes in the DNA sequence. Such epigenetic mechanisms are known to influence gene function in most complex organisms and include effects such as transposon function, chromosome imprinting, yeast mating type switching and telomeric silencing. In recent years, epigenetic effects have become a major focus of research activity. This monograph, edited by three well-known biologists from different specialties, is the first to review and synthesize what is known about these effects across all species, particularly from a molecular perspective, and will be of interest to everyone in the fields of molecular biology and genetics.

Expression and Meaning
Expression and Meaning

Author: John R. Searle

Publisher: Cambridge University Press

Published: 1979

Total Pages: 206

ISBN-13: 9780521313933

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A direct successor to Searle's Speech Acts (C.U.P. 1969), Expression and Meaning refines earlier analyses and extends speech-act theory to new areas including indirect and figurative discourse, metaphor and fiction.