Invertebrate Tissue Culture Methods
Invertebrate Tissue Culture Methods

Author: Jun Mitsuhashi

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 435

ISBN-13: 4431678751

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I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.

Tissue Culture
Tissue Culture

Author: Paul F. Jr. Kruse

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 897

ISBN-13: 0323142079

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Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Invertebrate Cell Culture
Invertebrate Cell Culture

Author: Karl Maramorosch

Publisher:

Published: 1997

Total Pages: 318

ISBN-13:

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This text aims to provide readers with a balanced cross-section of current developments within the research on invertebrate cell culture. Attention is focused on such topics as: the biochemistry and physiology of cultured invertebrate cells; aspects of virus infection; novel cultivation methods; assays of viruses affecting shrimp and insect cells; engineering of invertebrate cells for the production of baculovirus pesticides; application of microgravity to in vitro cell cultivation; and other aspects of biotechnology. The large body of information brings into focus the significant recent achievements in the laboratories of Africa, America, Europe and Asia.

Invertebrate Tissue Culture
Invertebrate Tissue Culture

Author: C Vago

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 456

ISBN-13: 0323149669

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Invertebrate Tissue Culture, Volume I, reviews advances in the use of tissue and organ culture in invertebrate research in physiology and pathology. It describes methods in invertebrate tissue culture, including organ culture techniques in liquid and gel media; aseptic rearing of invertebrates for tissue culture; and cell culture of organisms, ranging from Lepidoptera and Diptera to Coleoptera, Orthoptera, Dictyoptera, Hymenoptera, Hemiptera, Crustacea, Arachnida, Merostomacea, and mollusks. Organized into two sections encompassing 13 chapters, this volume begins with an overview of the general methodology in cell and organ cultures and their preparation from aseptic conditions. It then discusses methods for the examination of cultures, including those concerning ultrastructure studies by electron microscopy. The reader is also introduced to cell cultures obtained from different groups of invertebrates, with emphasis on peculiarities specific to each group; the morphology and physiology of cultured invertebrate cells; and cultivation and growth of cells. This book is a valuable resource for specialists in the field of invertebrate cell and organ culture.

Invertebrate Tissue Culture
Invertebrate Tissue Culture

Author: Edouard Kurstak

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 417

ISBN-13: 0323151469

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Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture comprises the proceedings of the IV International Conference on Invertebrate Tissue Culture, held on June 5-8, 1975 at Mont Gabriel, Quebec, Canada. The conference focuses on invertebrate organ, tissue, and cell culture, as well as cell culture limitations, pitfalls, and applications in medicine, biology, agriculture, neurophysiology, and studies of morphogenesis, differentiation, viruses, symbionts, and parasites. This reference material specifically provides information on sophisticated laboratory methods and on numerous utilizations of invertebrate cell culture techniques in medicine and biology. This book also elucidates the nutritional requirements and the establishment of cell lines. The study of viruses and protozoa of agricultural and forest importance is also shown. This book will be useful and stimulating to the readers and will provide in a single volume the results obtained in the diverse areas of research pursued by the leading exponents of invertebrate tissue culture from America, Europe, Asia, and Australia.

Cells and Tissues in Culture Methods, Biology and Physiology
Cells and Tissues in Culture Methods, Biology and Physiology

Author: E. N. Willmer

Publisher: Elsevier

Published: 2013-10-02

Total Pages: 843

ISBN-13: 1483277232

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Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures. The selection is a vital source of data for readers interested in the culture of cells and tissues.

Introduction to Cell and Tissue Culture
Introduction to Cell and Tissue Culture

Author: Jennie P. Mather

Publisher: Springer Science & Business Media

Published: 2007-08-20

Total Pages: 247

ISBN-13: 0585275718

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It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Invertebrate Tissue Culture
Invertebrate Tissue Culture

Author: Karl Maramorosch

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 409

ISBN-13: 0323151698

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Invertebrate Tissue Culture: Research Applications covers the broad field and status of basic research in invertebrate tissue culture. This book particularly discusses invertebrate cell growth and differentiation, cloning of established cell lines, the breakthrough in molluscan tissue culture, and the establishment of the first snail line. It also highlights topics on invertebrate endocrinology and ecdysone biosynthesis in vitro. This text describes the identification of distinct juvenile hormones from corpora allata and the production of peptide neurohormones by cultured insect brains. Some chapters elucidate the use of Drosophila discs in vitro to study gene activity sites, as well as the applications of insect tissue culture to the study of intracellular parasites, symbionts, and arboviruses. Discussions on insect pathogenic viruses in insect cell lines, extraneous contaminants in invertebrate cell cultures, uses of invertebrate cells in plant pathology, and a description of invertebrate cell lines complete this volume. This reference will be valuable to microbiologists, parasitologists, virologists, entomologists, geneticists, and medical researchers working in the field and to graduate students in related fields of biomedical research.

Plant Tissue Culture
Plant Tissue Culture

Author: Robert H. Smith

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 190

ISBN-13: 0323160476

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Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.