Invertebrate Cell System Applications

Invertebrate Cell System Applications

Author: Jun Mitsuhashi

Publisher: CRC Press

Published: 2018-01-18

Total Pages: 290

ISBN-13: 1351090747

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A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.


Invertebrate Cell System Applications

Invertebrate Cell System Applications

Author: Jun Mitsuhashi

Publisher: CRC Press

Published: 2019-08-08

Total Pages: 417

ISBN-13: 1351090755

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A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.


Invertebrate Cell System Applications, Volume II

Invertebrate Cell System Applications, Volume II

Author: Jun Mitsuhashi

Publisher: CRC Press

Published: 1989-05-31

Total Pages: 332

ISBN-13:

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A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.


Arthropod Cell Culture Systems

Arthropod Cell Culture Systems

Author: Karl Maramorosch

Publisher: CRC Press

Published: 1994-06-03

Total Pages: 250

ISBN-13: 9780849376429

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Invertebrate cell culture is increasingly being used in various areas of biological research. Research in cellular biology and pathology that previously depended primarily on in vitro investigations of vertebrate animal cell systems is now being conducted using invertebrate cells. Specialists and pioneers from the United States, Japan, Switzerland, Slovakia, and China have presented original contributions to create a well-balanced cross-section of current developments. Topics discussed include the preparation of cell culture media; cultivation of mosquito, lepidopteran, grasshopper, and tick cells; the application of such cells to mammalian and plant virus research; and diverse applications in medicine, biology, and agriculture. A special chapter devoted to the work of Japanese cell culture pioneers is also featured. All chapters are supported by tables, photographs, and up-to-date bibliographies.


Insect Cell Cultures

Insect Cell Cultures

Author: Just M. Vlak

Publisher: Springer Science & Business Media

Published: 2006-04-11

Total Pages: 303

ISBN-13: 0306468506

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A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.


Arthropod Cell Culture Systems

Arthropod Cell Culture Systems

Author: Karl Maramorosch

Publisher: CRC Press

Published: 2018-01-18

Total Pages: 247

ISBN-13: 1351078356

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* Discusses human, mammalian, insect and plant viruses in invertebrate cell culture systems* Addresses the commercial application of these systems in biotechnology and insect pest control* Brings together for the first time in over two decades the large body of information and significant achievements in the field


Invertebrate Cell Culture

Invertebrate Cell Culture

Author: Karl Maramorosch

Publisher:

Published: 1997

Total Pages: 318

ISBN-13:

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This text aims to provide readers with a balanced cross-section of current developments within the research on invertebrate cell culture. Attention is focused on such topics as: the biochemistry and physiology of cultured invertebrate cells; aspects of virus infection; novel cultivation methods; assays of viruses affecting shrimp and insect cells; engineering of invertebrate cells for the production of baculovirus pesticides; application of microgravity to in vitro cell cultivation; and other aspects of biotechnology. The large body of information brings into focus the significant recent achievements in the laboratories of Africa, America, Europe and Asia.


Invertebrate Tissue Culture Methods

Invertebrate Tissue Culture Methods

Author: Jun Mitsuhashi

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 435

ISBN-13: 4431678751

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I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.