Intracellular Thermometry with Fluorescent Molecular Thermometers
Intracellular Thermometry with Fluorescent Molecular Thermometers

Author: Seiichi Uchiyama

Publisher: John Wiley & Sons

Published: 2024-06-17

Total Pages: 357

ISBN-13: 3527350322

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Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find: Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

Intracellular Thermometry with Fluorescent Molecular Thermometers
Intracellular Thermometry with Fluorescent Molecular Thermometers

Author: Seiichi Uchiyama

Publisher: John Wiley & Sons

Published: 2024-04-03

Total Pages: 357

ISBN-13: 3527836853

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Intracellular Thermometry with Fluorescent Molecular Thermometers Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find: Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

Thermometry at the Nanoscale
Thermometry at the Nanoscale

Author: Luís Dias Carlos

Publisher: Royal Society of Chemistry

Published: 2016

Total Pages: 540

ISBN-13: 1849739048

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Covers the fundamentals of measuring temperature at the nanoscale, luminescence-based and non-luminescence based thermometry techniques, and applications.

Modern Thermodynamics
Modern Thermodynamics

Author: Dilip Kondepudi

Publisher: John Wiley & Sons

Published: 2014-12-31

Total Pages: 550

ISBN-13: 111837181X

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Modern Thermodynamics: From Heat Engines to Dissipative Structures, Second Edition presents a comprehensive introduction to 20th century thermodynamics that can be applied to both equilibrium and non-equilibrium systems, unifying what was traditionally divided into ‘thermodynamics’ and ‘kinetics’ into one theory of irreversible processes. This comprehensive text, suitable for introductory as well as advanced courses on thermodynamics, has been widely used by chemists, physicists, engineers and geologists. Fully revised and expanded, this new edition includes the following updates and features: Includes a completely new chapter on Principles of Statistical Thermodynamics. Presents new material on solar and wind energy flows and energy flows of interest to engineering. Covers new material on self-organization in non-equilibrium systems and the thermodynamics of small systems. Highlights a wide range of applications relevant to students across physical sciences and engineering courses. Introduces students to computational methods using updated Mathematica codes. Includes problem sets to help the reader understand and apply the principles introduced throughout the text. Solutions to exercises and supplementary lecture material provided online at http://sites.google.com/site/modernthermodynamics/. Modern Thermodynamics: From Heat Engines to Dissipative Structures, Second Edition is an essential resource for undergraduate and graduate students taking a course in thermodynamics.

Fluorescent polymers for sensing and imaging
Fluorescent polymers for sensing and imaging

Author: Seiichi Uchiyama

Publisher: MDPI

Published: 2020-12-29

Total Pages: 208

ISBN-13: 3039369407

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Nowadays, all scientists recognize that fluorescent probes play important roles in wide research areas, from chemistry to biology. By combining this fact with specific functional benefits from synthetic polymers, fluorescent polymeric probes are occasionally superior to small organic and inorganic fluorescent (or luminescent) probes in terms of sensitivity, robustness, and multiple functionality. The targets of fluorescent polymeric probes have extended from chemical species to physical parameter. This special issue is a platform for researches to develop a novel fluorescent polymeric probe and to establish a new analytical method using a conventional fluorescent polymeric probe. Related researches, e.g., fluorometric investigation of functional polymers, are also included.

Luminescence Thermometry
Luminescence Thermometry

Author: Miroslav Dramićanin

Publisher: Woodhead Publishing

Published: 2018-04-21

Total Pages: 304

ISBN-13: 0081020309

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Luminescence Thermometry: Methods, Materials, and Applications presents the state-of-the art applications of luminescence thermometry, giving a detailed explanation of luminescence spectroscopic schemes for the read-out of temperature, while also describing the diverse materials that are capable of sensing temperature via luminescence. Chapters cover the fundamentals of temperature, traditional thermometers and their figures of merit, a concise description of optical thermometry methods, luminescence and instrumentation, and an explanation of the ways in which increases in temperature quench luminescence. Additional sections focus on materials utilized for luminescence thermometry and the broad range of applications for luminescence thermometry, including temperature measurement at the nanoscale and the application of multifunctional luminescent materials. - Provides an overview of luminescence thermometry applications, including high-temperature, biomedical, nanoscale and multifunctional - Delves into luminescence thermometry by materials group, including Rare-earth and transition Metal Ion Doped, Semiconductors, Quantum Dots and Organic materials - Gives a concise introduction of the latest methods of temperature measurement, including luminescence spectroscopic schemes and methods of analysis

Nanomaterials for Magnetic and Optical Hyperthermia Applications
Nanomaterials for Magnetic and Optical Hyperthermia Applications

Author: Raluca Maria Fratila

Publisher: Elsevier

Published: 2018-11-30

Total Pages: 386

ISBN-13: 0128139293

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Nanomaterials for Magnetic and Optical Hyperthermia Applications focuses on the design, fabrication and characterization of nanomaterials (magnetic, gold and hybrid magnetic-gold nanoparticles) for in vitro and in vivo hyperthermia applications, both as standalone and adjuvant therapy in combination with chemotherapy. The book explores the potential for more effective cancer therapy solutions through the synergistic use of nanostructured materials as magnetic and optical hyperthermia agents and targeted drug delivery vehicles, while also discussing the challenges related to their toxicity, regulatory and translational aspects. In particular, the book focuses on the design, synthesis, biofunctionalization and characterization of nanomaterials employed for magnetic and optical hyperthermia. This book will be an important reference resource for scientists working in the areas of biomaterials and biomedicine seeking to learn about the potential of nanomaterials to provide hyperthermia solutions. - Explores the design of efficient nanomaterials for hyperthermia applications, allowing readers to make informed materials selection decisions - Discusses the biofunctionalization of a range of nanomaterials and their interaction with living systems - Provides an overview of the current clinical applications of nanomaterials in hyperthermia treatment

21st Century Nanoscience
21st Century Nanoscience

Author: Klaus D. Sattler

Publisher: CRC Press

Published: 2022-01-18

Total Pages: 4153

ISBN-13: 1351260553

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This 21st Century Nanoscience Handbook will be the most comprehensive, up-to-date large reference work for the field of nanoscience. Handbook of Nanophysics, by the same editor, published in the fall of 2010, was embraced as the first comprehensive reference to consider both fundamental and applied aspects of nanophysics. This follow-up project has been conceived as a necessary expansion and full update that considers the significant advances made in the field since 2010. It goes well beyond the physics as warranted by recent developments in the field. Key Features: Provides the most comprehensive, up-to-date large reference work for the field. Chapters written by international experts in the field. Emphasises presentation and real results and applications. This handbook distinguishes itself from other works by its breadth of coverage, readability and timely topics. The intended readership is very broad, from students and instructors to engineers, physicists, chemists, biologists, biomedical researchers, industry professionals, governmental scientists, and others whose work is impacted by nanotechnology. It will be an indispensable resource in academic, government, and industry libraries worldwide. The fields impacted by nanoscience extend from materials science and engineering to biotechnology, biomedical engineering, medicine, electrical engineering, pharmaceutical science, computer technology, aerospace engineering, mechanical engineering, food science, and beyond.

Fluorescence Applications in Biotechnology and Life Sciences
Fluorescence Applications in Biotechnology and Life Sciences

Author: Ewa M. Goldys

Publisher: John Wiley & Sons

Published: 2009-08-24

Total Pages: 398

ISBN-13: 0470083700

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A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.