HPLC of Proteins, Peptides and Polynucleotides
Author: Milton T. W. Hearn
Publisher:
Published: 1991
Total Pages: 800
ISBN-13:
DOWNLOAD EBOOKHPLC is High Performance Liquid Chromatography.
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High-Performance Liquid Chromatography of Peptides and Proteins
Author: Colin T. Mant
Publisher: CRC Press
Published: 2017-11-22
Total Pages: 887
ISBN-13: 1351440748
DOWNLOAD EBOOKThis book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Peptide and Protein Drug Delivery
Author: Vincent Lee
Publisher: CRC Press
Published: 2024-11-01
Total Pages: 913
ISBN-13: 1040284566
DOWNLOAD EBOOKThis reference/text covers fundamentals of peptide and protein drug delivery, including such considerations as synthesis, physical chemistry and biochemistry, analysis, proteolytic and transport constraints, pharmacokinetics, and pharmacodynamics; bioavailability from routes of administration, detai
Bioseparations of Proteins
Author: Ajit Sadana
Publisher: Elsevier
Published: 1997-11-27
Total Pages: 367
ISBN-13: 0080528147
DOWNLOAD EBOOKThis book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.Key Features* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:* detailed data on biological stability under various bioseparation conditions* extensive case studies from the literature on separation processes, validation, and economics* simplified analysis of protein refolding and inactivation mechanisms* consideration of adsorption theories and the effect of heterogeneity* coverage of both classical and novel bioseparation techniques, including chromatographic procedures
Federation Proceedings
Author: Federation of American Societies for Experimental Biology
Publisher:
Published: 1987-05
Total Pages: 746
ISBN-13:
DOWNLOAD EBOOKVols. for 1942- include proceedings of the American Physiological Society.
Chemistry, Structure and Function of Peptides
Author: Michel Marraud
Publisher: John Libbey Eurotext
Published: 1970
Total Pages: 656
ISBN-13: 9780861961511
DOWNLOAD EBOOKIncludes proceedings of the 2nd Forum on Peptides held in Nancy, 2 to 6 May 1988.
Current Research in Protein Chemistry
Author: Villafranc
Publisher: Elsevier
Published: 2012-12-02
Total Pages: 635
ISBN-13: 0323139442
DOWNLOAD EBOOKCurrent Research in Protein Chemistry: Techniques, Structure, and Function focuses on the techniques and methods used for determining the structure and function of proteins. Topics covered range from protein folding and stability to catalysis by chimeric proteins, amino acid and peptide analysis, applications of mass spectrometry to peptide and protein analysis, and protein sequencing. This book is divided into six sections encompassing 55 chapters. The first chapter describes a novel method for protein hydrolysis by means of microwave irradiation that uses Teflon-Pyrex tubes. This is followed by a discussion of the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow focus on mass spectrometric methods, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. The crystal structure of human interleukin-1alpha, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins are considered. The reader is also introduced to peptide mapping and internal sequencing of proteins from acrylamide gels, new approaches to covalent sequence analysis, alkaline denaturation of hemoglobin, and measurements of disulfide bond stabilities in protein folding intermediates. Students and researchers interested in protein chemistry will find this book extremely helpful.
Cumulated Index Medicus
Author:
Publisher:
Published: 1984
Total Pages: 1136
ISBN-13:
DOWNLOAD EBOOKHplc Of Biological Macro- Molecules, Revised And Expanded
Author: Karen M. Gooding
Publisher: CRC Press
Published: 2002-01-08
Total Pages: 806
ISBN-13: 9780203911594
DOWNLOAD EBOOKCompletely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.
