Techniques in Animal Cytogenetics
Techniques in Animal Cytogenetics

Author: Paul Popescu

Publisher: Springer Science & Business Media

Published: 2000-08-15

Total Pages: 260

ISBN-13: 9783540667377

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The theoretical background of cytogenetics as well as the practical methods to investigate chromosomes from Drosophila to mammals and humans are presented in this book. Theory and practical protocols on the following topics are included: Cell culture methods, chromosome preparation and banding techniques, radioactive and fluorescent in situ hybridisation (FISH) methods and their derivates (chromosome painting), meiotic chromosome methods using light and electronic microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes as well as flow and slit scan cytometry. The editors, all at INRA, played a prominent part in the development of cytogenetics. Paul Popescu, a specialist for domestic animals worked with gene mapping, Helene Hayes, together with Popescu showed the homology of bovine and human chromosome disorders, and Bernard Dutrilaux focused on human cytogenetics.

Insect Reproduction
Insect Reproduction

Author: S. R. Leather

Publisher: CRC Press

Published: 2018-01-18

Total Pages: 266

ISBN-13: 1351082051

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This book consisting of ten review chapters contributed by leading workers in their respective fields, from around the world, covers the whole subject of insect reproduction.It begins with the basic physiological questions of insect reproduction, moves on to discuss the new advances seen in the fields of behavioural and ecological mechanisms, and culminates by examining the recent work on evolutionary biology and its application in the field. Each chapter, although including a brief review of the basic seminal work, focuses mainly on the advances made within the last ten years and highlights those areas in which the respective authors see the greatest scope for further important advances

Insect Molecular Genetics
Insect Molecular Genetics

Author: Marjorie A. Hoy

Publisher: Academic Press

Published: 2013-04-09

Total Pages: 837

ISBN-13: 0240821319

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Insect Molecular Genetics, Third Edition, summarizes and synthesizes two rather disparate disciplines—entomology and molecular genetics. This volume provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools. The world of insect molecular genetics, once dominated by Drosophila, has become much more diverse, especially with the sequencing of multiple arthropod genomes (from spider mites to mosquitoes). This introduction includes discussion of honey bees, mosquitoes, flour beetles, silk moths, fruit flies, aphids, house flies, kissing bugs, cicadas, butterflies, tsetse flies and armyworms. This book serves as both a foundational text and a review of a rapidly growing literature. With fully revised and updated chapters, the third edition will be a valuable addition to the personal libraries of entomologists, geneticists, and molecular biologists. - Up-to-date references to important review articles, websites, and seminal citations in the disciplines - Well crafted and instructive illustrations integral to explaining the techniques of molecular genetics - Glossary of terms to help beginners learn the vocabulary of molecular biology

Insect Genomics
Insect Genomics

Author: Susan J. Brown

Publisher: Humana Press

Published: 2018-11-10

Total Pages: 237

ISBN-13: 9781493987740

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This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.

Protocols for Cytogenetic Mapping of Arthropod Genomes
Protocols for Cytogenetic Mapping of Arthropod Genomes

Author: Igor V. Sharakhov

Publisher: CRC Press

Published: 2014-10-28

Total Pages: 530

ISBN-13: 1466598158

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Arthropods are important to worldwide agriculture, food safety, human health, and energy production. Besides their practical significance, various species represent excellent model systems for biological investigations of evolution, development, physiology, reproduction, and social interaction. For these reasons, arthropod genomics is receiving increasing attention from researchers around the globe. Protocols for Cytogenetic Mapping of Arthropod Genomes is a collection of up-to-date, detailed protocols for physical chromosome mapping and their applications for studying genome organization and evolution in insects and ticks. This book brings together the expertise of cytogeneticists working on diverse groups of arthropods. Each chapter demonstrates approaches to tissue dissection, chromosome preparation, fluorescence in situ hybridization, and imaging. The book is a timely and complementary effort to the i5K initiative, which will obtain whole-genome sequences for 5,000 insect and related arthropod species. This comprehensive resource provides cytogeneticists with the necessary background and protocols to understand and develop chromosome-based genome assemblies from such whole-genome sequence data.

The Biology of Reproduction
The Biology of Reproduction

Author: Giuseppe Fusco

Publisher: Cambridge University Press

Published: 2019-10-10

Total Pages: 491

ISBN-13: 1108499856

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A look into the phenomena of sex and reproduction in all organisms, taking an innovative, unified and comprehensive approach.

Protocols for Cytogenetic Mapping of Arthropod Genomes
Protocols for Cytogenetic Mapping of Arthropod Genomes

Author: Igor V. Sharakhov

Publisher: CRC Press

Published: 2014-10-28

Total Pages: 522

ISBN-13: 1466598166

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Arthropods are important to worldwide agriculture, food safety, human health, and energy production. Besides their practical significance, various species represent excellent model systems for biological investigations of evolution, development, physiology, reproduction, and social interaction. For these reasons, arthropod genomics is receiving inc

Encyclopedia of Scale Insect Pests
Encyclopedia of Scale Insect Pests

Author: Takumasa Kondo

Publisher: CABI

Published: 2022-06-15

Total Pages: 636

ISBN-13: 1800620640

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Scale insects feed on plant juices and can easily be transported to new countries on live plants. They sometimes become invasive pests, costing billions of dollars in damage to crops worldwide annually, and farmers try to control them with toxic pesticides, risking environmental damage. Fortunately, scale insects are highly susceptible to control by natural enemies so biological control is possible. They have unique genetic systems, unusual metamorphosis, a broad spectrum of essential symbionts, and some are sources of commercial products like red dyes, shellac and wax. There is, therefore, wide interest in these unusual, destructive, beneficial, and abundant insects. The Encyclopedia of Scale Insect Pests is the most comprehensive work on worldwide scale insect pests, providing detailed coverage of the most important species (230 species in 26 families, 36% of the scale insect pest species known). Advice is provided on collection, preservation, slide-mounting, vouchering, and labelling of specimens, fully illustrated with colour photographs, diagrams and drawings.

Insect Development and Evolution
Insect Development and Evolution

Author: Bruce S. Heming

Publisher: Cornell University Press

Published: 2018-10-18

Total Pages: 464

ISBN-13: 1501720759

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Life scientists are increasingly drawn to the study of comparative evolutionary biology. Insect Development and Evolution is the first synthesis of knowledge of insect development within an evolutionary framework and the first to survey the genetic, molecular, and whole organism literature. Bruce S. Heming provides a detailed introduction to the embryonic and postembryonic development of insects. Topics include:* reproductive systems,* male and female gametogenesis,* sperm transfer and use,* fertilization,* sex determination,* parthenogenesis,* embryogenesis,* postembryogenesis,* hormones,* and the role of ontogeny in insect evolution.Summaries for each of these topics cover structural events; comparative aspects (inserted on a phylogeny of the insect orders); and hormonal, genetic, and molecular causal analyses.Insect Development and Evolution treats examples throughout the hexapods with frequent reference to the evolution and development of other invertebrates. It also compares insects to vertebrates and places insect development into context with fossil evidence and earth history. Heming's book will become an essential tool for students and teachers of entomology. It will also interest insect systematists and paleontologists, insect behavioral ecologists, insect pathologists, applied entomologists, developmental and invertebrate biologists, and all scientists who use Drosophila as a model organism.

The Evolution of Sex Determination
The Evolution of Sex Determination

Author: Leo Beukeboom

Publisher: OUP Oxford

Published: 2014-06-12

Total Pages: 455

ISBN-13: 019163140X

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Sexual reproduction is a fundamental aspect of life. It is defined by the occurrence of meiosis and the fusion of two gametes of different sexes or mating types. Sex-determination mechanisms are responsible for the sexual fate and development of sexual characteristics in an organism, be it a unicellular alga, a plant, or an animal. In many cases, sex determination is genetic: males and females have different alleles or different genes that specify their sexual morphology. In animals, this is often accompanied by chromosomal differences. In other cases, sex may be determined by environmental (e.g. temperature) or social variables (e.g. the size of an organism relative to other members of its population). Surprisingly, sex-determination mechanisms are not evolutionarily conserved but are bewilderingly diverse and appear to have had rapid turnover rates during evolution. Evolutionary biologists continue to seek a solution to this conundrum. What drives the surprising dynamics of such a fundamental process that always leads to the same outcome: two sex types, male and female? The answer is complex but the ongoing genomic revolution has already greatly increased our knowledge of sex-determination systems and sex chromosomes in recent years. This novel book presents and synthesizes our current understanding, and clearly shows that sex-determination evolution will remain a dynamic field of future research. The Evolution of Sex Determination is an advanced, research level text suitable for graduate students and researchers in genetics, developmental biology, and evolution.