In Vitro Embryogenesis in Higher Plants
In Vitro Embryogenesis in Higher Plants

Author: Maria Antonietta Germana

Publisher: Humana

Published: 2015-12-02

Total Pages: 0

ISBN-13: 9781493930609

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This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.

In Vitro Embryogenesis in Plants
In Vitro Embryogenesis in Plants

Author: Trevor A. Thorpe

Publisher: Springer Science & Business Media

Published: 1995-04-30

Total Pages: 582

ISBN-13: 9780792331490

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In vitro Embryogenesis in Plants is the first book devoted exclusively to this topic. As the ultimate demonstration of totipotency in plants, somatic and haploid embryogenesis is of vital importance to all those working on or interested in basic and applied aspects of plantlet information and regeneration. The text includes comprehensive reviews written by experts, on all facts of in vitro and in vivo embryogenesis. Some chapters deal with the morphogenic, structural and developmental, physiological and biochemical, and molecular biological aspects of the subject. Chapters are also devoted to haploid embryogenesis, asexual embryogenesis in nature, zygotic embryogenesis, and zygotic embryo culture. Detailed tables summarizing successful somatic embryogenesis in all vascular plants are also included. This book, therefore, brings together previously scattered information to provide an indispensable reference book for both active researchers, graduate students and anyone interested in this aspect of tissue culture technology and plant development.

Polarity in Plants
Polarity in Plants

Author: K. Lindsey

Publisher: John Wiley & Sons

Published: 2004

Total Pages: 368

ISBN-13: 9780849323447

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The establishment of polarity is a fundamental feature in eukaryotic development. Polarity in Plants provides an account of current research into the mechanisms by which polarity is generated at the level of the cell, organ and organism in plants, drawing especially on recent work with model organisms. The emphasis is on the use of the techniques of molecular genetics to dissect molecular mechanisms. This is the first volume to bring together the diverse aspects of polarity in plant development.

In Vitro Culture of Higher Plants
In Vitro Culture of Higher Plants

Author: R.L.M Pierik

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 346

ISBN-13: 9401157502

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In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

In Vitro Haploid Production in Higher Plants
In Vitro Haploid Production in Higher Plants

Author: S. Mohan Jain

Publisher: Springer Science & Business Media

Published: 1996-06-30

Total Pages: 380

ISBN-13: 9780792335771

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The 18 chapters making up In Vitro Haploid Production in Higher Plants are divided into two sections. Section 1 (eight chapters) covers historical and fundamental aspects of haploidy in crop improvement. Section 2 deals with methods of haploid production, including anther culture, micropore culture, ovary culture, pollination with irradiated pollen, in vitro pollination, and special culture techniques, including polyhaploid production in the Triticeae by sexual hybridization, the influence of ethylene and gelling agents on anther culture, conditional lethal markers, and methods of chromosome doubling.

In Vitro Haploid Production in Higher Plants
In Vitro Haploid Production in Higher Plants

Author: S. Mohan Jain

Publisher: Springer Science & Business Media

Published: 2013-03-09

Total Pages: 363

ISBN-13: 9401718601

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The 18 chapters making up In Vitro Haploid Production in Higher Plants are divided into two sections. Section 1 (eight chapters) covers historical and fundamental aspects of haploidy in crop improvement. Section 2 deals with methods of haploid production, including anther culture, micropore culture, ovary culture, pollination with irradiated pollen, in vitro pollination, and special culture techniques, including polyhaploid production in the Triticeae by sexual hybridization, the influence of ethylene and gelling agents on anther culture, conditional lethal markers, and methods of chromosome doubling.

Sexual Plant Reproduction
Sexual Plant Reproduction

Author: Mauro Cresti

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 241

ISBN-13: 3642776779

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In recent years there has been a growing awareness of the importance of reproductive biology to crop production and there has been a tremendous increase in research on reproductive structures of higher plants. Presented here is a wide information of different aspects of micro- and macrosporogenesis, pollen-stigma interaction and recognition, pollen tube growth, cytoskeleton, in vitro and in vivo gamete fusion, and incompatibility. The most advanced techniques employed in studies on reproductive biology of higher plants are described in detail.

Plant Embryogenesis
Plant Embryogenesis

Author: María F. Suárez

Publisher: Springer Science & Business Media

Published: 2008

Total Pages: 184

ISBN-13: 1588299317

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Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips. Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism. Provides the most comprehensive collection of protocols on plan embryo development.

Somatic Embryogenesis: Fundamental Aspects and Applications
Somatic Embryogenesis: Fundamental Aspects and Applications

Author: Víctor M Loyola-Vargas

Publisher: Springer

Published: 2016-06-06

Total Pages: 503

ISBN-13: 331933705X

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This book discusses basic and applied aspects of somatic embryogenesis, one of the most powerful tools in plant biotechnology. It is divided into three parts; Part I includes topics such as the history of this research field, how differentiated plant cells can (re)acquire totipotency, molecular features, as well as the epigenetics and proteomics of somatic embryogenesis. Part II covers the somatic embryogenesis of different crops, such as Agave spp. maize, Cocos nucifera, Bixa orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and Arabidopsis thaliana. Various applications, like scale-up propagation and genetic engineering are discussed in detail in Part III. The book will appeal to plant scientists, plant breeders and experts working in industry.

Somatic Embryogenesis in Woody Plants
Somatic Embryogenesis in Woody Plants

Author: S.M. Jain

Publisher: Springer Science & Business Media

Published: 2013-11-11

Total Pages: 743

ISBN-13: 940173030X

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The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environ mental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Gene rally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taedb), Sandalwood (Santalum album), Citrus, mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propagation for large-scale production of plants or "emblings" or somatic seedlings; b) protoplast work; c) cryopreservation; d) genetic transformation; and e) synthetic or artificial or manufactured seed production.