Plant Cell Culture Protocols
Plant Cell Culture Protocols

Author: Víctor M. Loyola-Vargas

Publisher: Humana Press

Published: 2012-05-10

Total Pages: 430

ISBN-13: 9781617798177

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Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Applications of Plant Cell and Tissue Culture
Applications of Plant Cell and Tissue Culture

Author: Gregory R. Bock

Publisher: John Wiley & Sons

Published: 2008-04-30

Total Pages: 280

ISBN-13: 0470513667

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This work deals with basic plant physiology and cytology, and addresses the practical exploitation of plants, both as crops and as sources of useful compounds produced as secondary metabolites. Covers problems of commercial exploitation, socio-legal aspects of genetic engineering of crop plants, and of the difficulties of marketing natural compunds produced by cells under artificial conditions.

Plant Cell and Tissue Culture
Plant Cell and Tissue Culture

Author: Indra K. Vasil

Publisher: Springer Science & Business Media

Published: 2013-03-09

Total Pages: 593

ISBN-13: 9401726817

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Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture

Author: Oluf Gamborg

Publisher: Springer Science & Business Media

Published: 2013-06-29

Total Pages: 367

ISBN-13: 3642790488

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This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Plant Tissue Culture
Plant Tissue Culture

Author: Robert H. Smith

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 190

ISBN-13: 0323160476

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Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Plant Cell Culture
Plant Cell Culture

Author: Michael R. Davey

Publisher: John Wiley & Sons

Published: 2010-05-20

Total Pages: 358

ISBN-13: 9780470686515

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The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

In Vitro Cultivation of Plant Cells
In Vitro Cultivation of Plant Cells

Author: Open Universiteit (Heerlen, Netherlands)

Publisher: Butterworth-Heinemann

Published: 1993

Total Pages: 228

ISBN-13:

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This text aims to provide the essential knowledge of the core processes involved in the cultivation of plant cells and tissues in vitro thereby enabling readers to understand and contribute to the practical application of these techniques. The text begins by introducing the key terms used in plant tissue culture and provides an overview of the range and objectives of plant tissue culture studies. Subsequent chapters provide knowledge of the processes of the major techniques developed within the framework of the perspectives described in the introductory chapter. The important issues of media formulation and preparation are described together with discussion of the influence of plant growth regulators on the growth and development of plant cell systems in vitro.

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences
Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

Author: Saurabh Bhatia

Publisher: Academic Press

Published: 2015-07-22

Total Pages: 452

ISBN-13: 0128024984

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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions