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Author: M. A. Hayat
Publisher: CRC Press
Published: 2024-11-01
Total Pages: 337
ISBN-13: 1040290531
DOWNLOAD EBOOKThis book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.
Author: Gerhard W. Hacker
Publisher: CRC Press
Published: 2002-04-17
Total Pages: 264
ISBN-13: 9780849313929
DOWNLOAD EBOOKImmunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know about the various staining methods, their useful applications, and the advantages and drawbacks of each process. Gold and Silver Staining: Techniques in Molecular Morphology provides a timely description of approaches, methods, protocols, and applications. The contributors cover the latest developments and a wide range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. Some of the authors are "living legends" in the field and bring their expertise and experience to this fine collaboration. Written in one consistent style, each chapter includes a concise, but comprehensive introduction, step-by-step protocols with technical hints, and a discussion of results and critical steps. What differentiates this book from all others like it, is the status of the editors, who have worked on this technique from its inception and have produced innumerable publications on the topic. The other distinguishing feature is that all of the contributors are amongst the absolute foremost leaders from the United States and Europe. Gold and Silver Staining: Techniques in Molecular Morphology presents a complete overview and detailed descriptions of this technique that allows the visualization of molecules that have never been localized before and with hitherto unknown sensitivity. Not only does this book provide an excellent review of this field, but it also serves as a lab manual for those who want to carry out this technique in their laboratory and clinical work. Armed with this information, advances in this powerful field of research will ensue.
Author: M. A. Hayat
Publisher: CRC Press
Published: 1995-08-03
Total Pages: 344
ISBN-13: 9780849324499
DOWNLOAD EBOOKThis book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.
Author: Gerhard W. Hacker
Publisher: CRC Press
Published: 2002-04-17
Total Pages: 265
ISBN-13: 1420040235
DOWNLOAD EBOOKImmunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a
Author: Alexander D. Hyatt
Publisher: CRC Press
Published: 1992-11-10
Total Pages: 476
ISBN-13: 9780849367595
DOWNLOAD EBOOKThis book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.
Author:
Publisher: Elsevier
Published: 2012-12-02
Total Pages: 517
ISBN-13: 0323139027
DOWNLOAD EBOOKSince its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. - Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books - Problems and limitations of techniques are discussed - Guides users to avoid problems and choose the correct procedures for specific applications - Contributors are eminent authorities in their fields
Author: Jiang Gu
Publisher: Springer Science & Business Media
Published: 1997-03-20
Total Pages: 308
ISBN-13: 9780817639570
DOWNLOAD EBOOKAnalytical Morphology is a new discipline dealing with the analysis of cell and tissue components using analytical maneuvers that go beyond those of conventional morphology and histopathology. It utilizes new advances in other disciplines, such as immunology, molecular biology, laser technology, microwave technology and computer science, to highlight cellular components such as peptides, proteins, or nucleotide sequences through chemical, physical, immunological, or molecular biological reactions. These methods include immunocytochemistry, in situ hybridization, in situ PCR, antigen retrieval, image analysis, and the like. The book is intended for morphological scientists, pathologists, advanced students, and technicians who are interested in keeping abreast of this rapidly emerging discipline.
Author: Runjan Chetty
Publisher: Cambridge University Press
Published: 2016-11-28
Total Pages: 525
ISBN-13: 1316592065
DOWNLOAD EBOOKProviding a unique A-Z guide to antibodies for immunohistology, this is an indispensable source for pathologists to ensure the correct application of immunohistochemistry in daily practice. Each entry includes commercial sources, clones, descriptions of stained proteins/epitopes, the full staining spectrum of normal and tumor tissues, staining pattern and cellular localization, the range of conditions of immunoreactivity, and pitfalls of the antibody's immunoprofile, giving pathologists a truly thorough quick-reference guide to sources, preparation and applications of specific antibodies. Appendices provide useful quick-reference tables of antibody panels for differential diagnoses, as well as summaries of diagnostic applications. Expanded from previous editions with over forty new entries, this handbook for diagnostic, therapeutic, prognostic and research applications of antibodies is an essential desktop book for practicing pathologists as well as researchers, residents and trainees.
Author: Laszlo Zaborszky
Publisher: Springer Science & Business Media
Published: 2013-03-09
Total Pages: 429
ISBN-13: 1475720556
DOWNLOAD EBOOKThis new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.
Author: Roland A. Fleck
Publisher: John Wiley & Sons
Published: 2019-04-29
Total Pages: 741
ISBN-13: 1118654064
DOWNLOAD EBOOKThe go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
