Immuno-Gold Electron Microscopy in Virus Diagnosis and Research
Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

Author: Alexander D. Hyatt

Publisher: CRC Press

Published: 1992-11-10

Total Pages: 476

ISBN-13: 9780849367595

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This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Electron Microscopy in Viral Diagnosis
Electron Microscopy in Viral Diagnosis

Author: Erskine L. Palmer

Publisher: CRC Press

Published: 2019-07-23

Total Pages: 202

ISBN-13: 1351080091

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This text on electron microscopy in viral diagnosis is an invaluable reference investigators interested in the detection of viruses or viral subcomponents in liquid preparations or in thin sectioned cells. It contains an extensive collection of negative stain, thin section, and immunolabelled electron micrographs useful for reference viral diagnosis. The salient features of the replication of many virus families are presented in schematic form and all viruses now known to cause disease in humans, including the recently recognized human retroviruses, are described in separate chapter.

Diagnostic Electron Microscopy
Diagnostic Electron Microscopy

Author: John Stirling

Publisher: John Wiley & Sons

Published: 2013-01-22

Total Pages: 492

ISBN-13: 1119973996

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Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.

Immunogold-Silver Staining
Immunogold-Silver Staining

Author: M. A. Hayat

Publisher: CRC Press

Published: 1995-08-03

Total Pages: 344

ISBN-13: 9780849324499

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This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

Colloidal Gold
Colloidal Gold

Author: M. A. Hayat

Publisher:

Published: 1989

Total Pages: 421

ISBN-13: 9780123339294

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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field.

The Transmission Electron Microscope
The Transmission Electron Microscope

Author: Khan Maaz

Publisher: BoD – Books on Demand

Published: 2015-09-02

Total Pages: 362

ISBN-13: 9535121502

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This book The Transmission Electron Microscope abundantly illustrates necessary insight and guidance of this powerful and versatile material characterization technique with complete figures and thorough explanations. The second edition of the book presents deep understanding of new techniques from introduction to advance levels, covering in-situ transmission electron microscopy, electron and focused ion beam microscopy, and biological diagnostic through TEM. The chapters cover all major aspects of transmission electron microscopy and their uses in material characterization with special emphasis on both the theoretical and experimental aspects of modern electron microscopy techniques. It is believed that this book will provide a solid foundation of electron microscopy to the students, scientists, and engineers working in the field of material science and condensed matter physics.

Modern Electron Microscopy in Physical and Life Sciences
Modern Electron Microscopy in Physical and Life Sciences

Author: Milos Janecek

Publisher: BoD – Books on Demand

Published: 2016-02-18

Total Pages: 302

ISBN-13: 9535122525

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This book brings a broad review of recent global developments in theory, instrumentation, and practical applications of electron microscopy. It was created by 13 contributions from experts in different fields of electron microscopy and technology from over 20 research institutes worldwide.

Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences
Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences

Author: Julian E. Beesley

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 277

ISBN-13: 146120139X

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Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.