Immobilization of Enzymes and Cells
Immobilization of Enzymes and Cells

Author: Jose M. Guisan

Publisher: Humana

Published: 2021-01-29

Total Pages: 495

ISBN-13: 9781071602171

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This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.

Carrier-bound Immobilized Enzymes
Carrier-bound Immobilized Enzymes

Author: Linqiu Cao

Publisher: John Wiley & Sons

Published: 2006-05-12

Total Pages: 578

ISBN-13: 3527607080

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The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes. A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.

Enzyme Immobilization
Enzyme Immobilization

Author: Alka Dwevedi

Publisher: Springer

Published: 2016-09-15

Total Pages: 141

ISBN-13: 3319414186

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This book covers the latest developments in enzyme immobilization with its wide applications, such as for industry, agriculture, medicine, and the environment. Topics covered include basics of enzyme immobilization, its implication in therapeutics and disease diagnostics, and its significance in solving environmental problems. This is an ideal book for researchers, graduate and postgraduate students, as well as scientists in industry, agriculture and health sectors. This book is a complete summary of enzyme immobilization and also thoroughly covers all the latest research. This book covers: The last one-hundred years of innovative research done in enzyme immobilization Recent developments in immobilization techniques, such as types of matrices, immobilization methods, and linking agents, as well as enzyme immobilization without any matrices and its properties The physiological and industrial significance of enzymes from plants and the implementation of immobilized enzymes in the treatment of waste water and polluted air Biomedical and bioanalytical applications of immobilized enzymes

Immobilized Enzymes For Industrial Reactors
Immobilized Enzymes For Industrial Reactors

Author: Ralph Messing

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 249

ISBN-13: 0323141323

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Immobilized Enzymes for Industrial Reactors aims to guide the engineer and scientist along the path toward the industrial application of immobilized enzymes. It is necessary to identify the hazards and pitfalls that will be encountered, not only in the initial research efforts, but also during the final engineering phases of a commercial program. Each contributing factor in an immobilized enzyme system will be scrutinized by the authors in an effort to accomplish the overall objectives. This book comprises 10 chapters, with the first being an introduction to and general history of immobilized enzymes. The next chapters go on to discuss basic enzymology; controlled-pore glasses for enzyme immobilization; carriers; immobilization by adsorption and inorganic bridge formation; immobilization by covalent attachment and by entrapment; characteristics of free vs. immobilized enzymes; immobilized coenzymes; design and operation of immobilized enzyme reactors; and applications of immobilized enzymes. This book will be of interest to practitioners in the fields of chemistry and engineering.

Immobilization of Enzymes and Cells
Immobilization of Enzymes and Cells

Author: Jose M. Guisan

Publisher: Humana

Published: 2016-08-27

Total Pages: 0

ISBN-13: 9781493959884

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For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

Catalyst Immobilization
Catalyst Immobilization

Author: Maurizio Benaglia

Publisher: John Wiley & Sons

Published: 2020-04-06

Total Pages: 492

ISBN-13: 3527345094

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A comprehensive resource on techniques and applications for immobilizing catalysts Catalyst Immobilization: Methods and Applications covers catalyst immobilization topics including technologies, materials, characterization, chemical activity, and recyclability. The book also presents innovative applications for supported catalysts, such as flow chemistry and machine-assisted organic synthesis. Written by an international panel of expert contributors, this book outlines the general principles of catalyst immobilization and explores different types of supports employed in catalyst heterogenization. The book?s chapters examine the immobilization of chiral organocatalysts, reactions in flow reactors, 3D printed devices for catalytic systems, and more. Catalyst Immobilization offers a modern vision and a broad and critical view of this exciting field. This important book: -Offers a guide to supported and therefore recyclable catalysts, which is one of the most important tools for developing a highly sustainable chemistry -Presents various immobilization techniques and applications -Explores new trends, such as 3D printed devices for catalytic systems -Contains information from a leading international team of authors Written for catalytic chemists, organic chemists, process engineers, biochemists, surface chemists, materials scientists, analytical chemists, Catalyst Immobilization: Methods and Applications presents the latest developments and includes a review of the innovative trends such as flow chemistry, reactions in microreactors, and beyond.

Immobilization of Enzymes and Cells
Immobilization of Enzymes and Cells

Author: José M. Guisán

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 447

ISBN-13: 1597450537

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Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.

Biocatalysis
Biocatalysis

Author: W.-D. Fessner

Publisher: Springer Science & Business Media

Published: 2003-09-05

Total Pages: 273

ISBN-13: 3540681167

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Here, leading contributors from the forefront of this exciting technology present authoritative and timely reviews on the state of the art of biocatalysis. They cover the whole spectrum from the discovery of novel enzymes - by modern screening, evolutionary or immunological approaches - through immobilization techniques for technical processes, to their use in the asymmetric synthesis of important target compounds.

Immobilized Enzymes in Medicine
Immobilized Enzymes in Medicine

Author: Vladimir P. Torchilin

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 214

ISBN-13: 3642758215

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The application of immobilized enzymes in medicine is the main objective of this book. The author reviews natural and synthetic carriers for enzyme immobilization, chemistry of enzyme binding, and in-vitro and in-vivo properties of immobilized enzymes. Four chapters are dedicated to clinical use of immobilized enzymes.

Green Synthesis of Nanomaterials for Bioenergy Applications
Green Synthesis of Nanomaterials for Bioenergy Applications

Author: Neha Srivastava

Publisher: John Wiley & Sons

Published: 2020-11-09

Total Pages: 266

ISBN-13: 1119576814

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An authoritative summary of the quest for an environmentally sustainable synthesis process of nanomaterials and their application for environmental sustainability Green Synthesis of Nanomaterials for Bioenergy Applications is an important guide that provides information on the fabrication of nanomaterial and the application of low cost, green methods. The book also explores the impact on various existing bioenergy approaches. Throughout the book, the contributors—noted experts on the topic—offer a reliable summary of the quest for an environmentally sustainable synthesis process of nanomaterials and their application to the field of environmental sustainability. The green synthesis of nanoparticles process has been widely accepted as a promising technique that can be applied to a variety of fields. The green nanotechnology-based production processes to fabricate nanomaterials operates under green conditions without the intervention of toxic chemicals. The book’s exploration of more reliable and sustainable processes for the synthesis of nanomaterials, can lead to the commercial application of the economically viability of low-cost biofuels production. This important book: Summarizes the quest for an environmentally sustainable synthesis process of nanomaterials for their application to the field of environmental sustainability Offers an alternate, sustainable green energy approach that can be commercially implemented worldwide Covers recent approaches such as fabrication of nanomaterial that apply low cost, green methods and examines its impact on various existing bioenergy applications Written for researchers, academics and students of nanotechnology, nanosciences, bioenergy, material science, environmental sciences, and pollution control, Green Synthesis of Nanomaterials for Bioenergy Applications is a must-have guide that covers green synthesis and characterization of nanomaterials for cost effective bioenergy applications.