Imaging Life
Imaging Life

Author: Lawrence R. Griffing

Publisher: John Wiley & Sons

Published: 2023-04-25

Total Pages: 548

ISBN-13: 1119949203

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Hands-on resource to understand and successfully process biological image data In Imaging Life: Image Acquisition and Analysis in Biology and Medicine, distinguished biologist Dr. Lawrence R. Griffing delivers a comprehensive and accessible exploration of scientific imaging, including but not limited to the different scientific imaging technologies, image processing, and analysis. The author discusses technical features, challenges, and solutions of the various imaging modalities to obtain the best possible image. Divided into three sections, the book opens with the basics such as the various image media, their representation and evaluation. It explains in exceptional detail pre- and postprocessing of an image. The last section concludes with common microscopic and biomedical imaging modalities in light of technical limitations and solutions to achieve the best possible image acquisition of the specimen. Imaging Life: Image Acquisition and Analysis in Biology and Medicine is written specifically for readers with limited mathematical and programming backgrounds and includes tutorials on image processing in relevant chapters. It also contains exercises in the use of popular, open-source software. A thorough introduction to imaging methods, technical features, challenges, and solutions to successfully capture biological images Offers tutorials on image processing using open-source software in relevant chapter Discusses details of acquisition needs and image media covering pixels, pixel values, contrast, tonal range, and image formats In-depth presentation of microscopic and biomedical imaging modalities Perfect for professionals and students in the biological sciences and engineering, Imaging Life: Image Acquisition and Analysis in Biology and Medicine is an ideal resource for research labs, biotech companies, and equipment vendors.

Positive Imaging
Positive Imaging

Author: Norman Vincent Peale

Publisher: Open Road Media

Published: 2015-09-29

Total Pages: 156

ISBN-13: 1504023250

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The #1 New York Times–bestselling author and self-help expert combines visualization and prayer to enhance the power of positive thinking. Norman Vincent Peale’s groundbreaking self-help classic, The Power of Positive Thinking, has dramatically transformed countless lives throughout the world with its powerful message of constructive affirmation. Positive Imaging builds on the principles originally presented in Dr. Peale’s life-changing, multi-million-copy bestseller, offering step-by-step guidance that will help you break through the barriers that stand in the way of achieving the harmony, happiness, and success you so fervently desire. In this essential volume, Dr. Peale takes the positive thinking idea a step further. By employing a potent mental process called “imaging,” you can eliminate problems and take firm control of your life. Keeping a clear and vivid picture of a desired goal in your mind until it becomes part of your subconscious will help you actualize your objectives by releasing previously untapped inner energies. With Positive Imaging you can banish fear and loneliness, strengthen and gain new confidence in your interpersonal relationships, improve your health, and eliminate your financial worries. The path to mental and physical wellness, spiritual well-being, and overall success in life is opening up right in front of you—let Dr. Peale show you the way.

Imaging Life
Imaging Life

Author: Gary C. Howard

Publisher: Oxford University Press

Published: 2014-07-30

Total Pages: 505

ISBN-13: 0199718172

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This volume provides an overview of a variety of approaches to biological image analysis, which allow for the study of living organisms at all levels of complexity and organization. These organisms range from individual macromolecules to subcellular and cellular volumes, tissues and microbial communities. Such a "systems biology" understanding of life requires the combination of a variety of imaging techniques, and with it an in-depth understanding of their respective strengths and limitations, as well as their intersection with other techniques. Howard, Brown, and Auer show us that the integration of these imaging techniques will allow us to overcome the reductionist approach to biology that dominated the twentieth century, which was aimed at examining the physical and chemical properties of life's constituents, one macromolecule at a time. However, while based on the laws of physics and chemistry, life is not simply a set of chemical reactions and physical forces; it features an exquisite spatiotemporal organization that allows an inconceivably large number of chemical processes to coexist, refined by billions of years of evolutionary experimentation. And yet, many fundamental questions remain largely unanswered; Imaging Life argues that we are just now beginning to address the spatiotemporal organizational component of living processes. "Imaging" is needed in order to reveal the spatiotemporal relationships between components, and thus to understand organizational guiding principles of living systems. Only through imaging will we be able to decipher the mechanisms and the marvelous organization that enable and sustain the mystery of life. Imaging Life shows us how biology is beginning to do just that.

Imaging Life After Death
Imaging Life After Death

Author: Kathleen Fischer

Publisher: Paulist Press

Published: 2004

Total Pages: 176

ISBN-13: 9780809142446

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Using sources such as religion, literature, science, psychology, and personal experience, this theologian examines the possible answers to three questions fundamental to belief in life after death: What survives death? Do relationships continue beyond death? How does life now relate to life then. Original.

Medical Imaging Systems
Medical Imaging Systems

Author: Andreas Maier

Publisher: Springer

Published: 2018-08-02

Total Pages: 263

ISBN-13: 3319965204

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This open access book gives a complete and comprehensive introduction to the fields of medical imaging systems, as designed for a broad range of applications. The authors of the book first explain the foundations of system theory and image processing, before highlighting several modalities in a dedicated chapter. The initial focus is on modalities that are closely related to traditional camera systems such as endoscopy and microscopy. This is followed by more complex image formation processes: magnetic resonance imaging, X-ray projection imaging, computed tomography, X-ray phase-contrast imaging, nuclear imaging, ultrasound, and optical coherence tomography.

Live Cell Imaging
Live Cell Imaging

Author: Robert D. Goldman

Publisher: CSHL Press

Published: 2005

Total Pages: 658

ISBN-13: 9780879696832

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Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

Multiphoton Microscopy and Fluorescence Lifetime Imaging
Multiphoton Microscopy and Fluorescence Lifetime Imaging

Author: Karsten König

Publisher: Walter de Gruyter GmbH & Co KG

Published: 2018-01-22

Total Pages: 785

ISBN-13: 311043007X

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This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Molybdenum-99 for Medical Imaging
Molybdenum-99 for Medical Imaging

Author: National Academies of Sciences, Engineering, and Medicine

Publisher: National Academies Press

Published: 2016-11-28

Total Pages: 264

ISBN-13: 0309445310

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The decay product of the medical isotope molybdenum-99 (Mo-99), technetium-99m (Tc-99m), and associated medical isotopes iodine-131 (I-131) and xenon-133 (Xe-133) are used worldwide for medical diagnostic imaging or therapy. The United States consumes about half of the world's supply of Mo-99, but there has been no domestic (i.e., U.S.-based) production of this isotope since the late 1980s. The United States imports Mo-99 for domestic use from Australia, Canada, Europe, and South Africa. Mo-99 and Tc-99m cannot be stockpiled for use because of their short half-lives. Consequently, they must be routinely produced and delivered to medical imaging centers. Almost all Mo-99 for medical use is produced by irradiating highly enriched uranium (HEU) targets in research reactors, several of which are over 50 years old and are approaching the end of their operating lives. Unanticipated and extended shutdowns of some of these old reactors have resulted in severe Mo-99 supply shortages in the United States and other countries. Some of these shortages have disrupted the delivery of medical care. Molybdenum-99 for Medical Imaging examines the production and utilization of Mo-99 and associated medical isotopes, and provides recommendations for medical use.

Advanced Imaging in Biology and Medicine
Advanced Imaging in Biology and Medicine

Author: Ch.W. Sensen

Publisher: Springer Science & Business Media

Published: 2008-12-03

Total Pages: 447

ISBN-13: 3540689931

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A picture says more than a thousand words. This is something that we all know to be true. Imaging has been important since the early days of medicine and bi- ogy, as seen in the anatomical studies of Leonardo Da Vinci or Andreas Vesalius. More than 100 years ago, the ?rst noninvasive imaging technologies, such as K- rad Roentgen’s X-ray technology,were applied to the medical ?eld—and while still crude—revolutionized medical diagnosis. Today, every patient will be exposed to some kind of advanced imaging technology such as medical resonance imaging, computed tomography or four-dimensional ultrasound during their lifetime. Many diseases, such as brain tumors, are initially diagnosed solely by imaging, and most of the surgical planning relies on the patient imagery. 4D ultrasound is available to expecting parents who wish to create unique early memories of the new baby, and it may soon be used for the morphometric diagnosis of malformations that may one day be treatable—inutero! Light and electron microscopy are unequal brethren, which have contributed to most of our knowledge about the existence and organization of cells, tissues and microorganisms. Every student of biology or medicine is introduced to the fascinating images of the microcosm. New advances have converted these im- ing technologies, which were considered by many to be antiquated, into powerful tools for research in systems biology and related ?elds.