Hyperthermophilic Enzymes, Part C
Hyperthermophilic Enzymes, Part C

Author:

Publisher: Elsevier

Published: 2001-06-05

Total Pages: 561

ISBN-13: 0080496865

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.This volume and its companions (Volumes 330 and 331) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include redox and thiol-dependent proteins, nucleic acid modifying enzymes, and protein stability from biochemical and biophysical standpoints.

Hyperthermophilic Enzymes
Hyperthermophilic Enzymes

Author: Michael W. W. Adams

Publisher: Academic Press

Published: 2001

Total Pages: 562

ISBN-13: 0121822354

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This volume and its companions (Volumes 330 and 331) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include redox and thiol-dependent proteins, nucleic acid modifying enzymes, and protein stability from biochemical and biophysical standpoints.

Chromatin and Chromatin Remodeling Enzymes Part C
Chromatin and Chromatin Remodeling Enzymes Part C

Author: Carl Wu

Publisher: Elsevier

Published: 2004-04-07

Total Pages: 585

ISBN-13: 0080497152

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DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the chromatin remodelling enzymes play an important role in gene transcription. - Genetic assays of chromatin modification and remodeling - Histone modifying enzymes - ATP-dependent chromatin remodeling enzymes

Biothermodynamics, Part C
Biothermodynamics, Part C

Author:

Publisher: Academic Press

Published: 2010-12-28

Total Pages: 408

ISBN-13: 0123812690

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In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. Biothermodynamics, Part C presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an “energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well. Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research Offers step-by-step lab instructions, including necessary equipment, from a global research community

G Protein Pathways
G Protein Pathways

Author: Ravi Iyengar

Publisher: Academic Press

Published: 2002

Total Pages: 696

ISBN-13: 012182246X

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This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for studying the structure and function of G proteins are important not only to those with specific research interests in them, but also endocrinologists and pharmacologists conducting research on signaling mechanisms that are increasingly understood to interact with G proteins.

Thermophilic Bacteria
Thermophilic Bacteria

Author: Jakob K. Kristjansson

Publisher: CRC Press

Published: 1991-11-22

Total Pages: 238

ISBN-13: 9780849352393

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Thermophilic Bacteria is a comprehensive volume that describes all major bacterial groups that can grow above 60-65°C (excluding the Archaea). Over 60 different species of aerobic and anaerobic thermophilic bacteria are covered. Isolation, growth methods, characterization and identification, ecology, metabolism, and enzymology of thermophilic bacteria are examined in detail, and an extensive compilation of recent biotechnological applications and the properties of many thermostable enzymes are also included. Major topics discussed in the book include a general review on thermophilic bacteria and archaea; heterotropic bacilli; the genus Thermus; new and rare genera of aerobic heterophophs, such as Saccharococcus, Rhodothermus, and Scotohermus; aerobic chemolithoautotrophic thermophilic bacteria; obligately anaerobic thermophilic bacteria; and hyperthermophilic Thermotogales and thermophilic phototrophs. Extensive bibliographies are also provided for each chapter. The vast amount of information packed into this one volume makes it essential for all microbiologists, biochemists, molecular biologists, and students interested in the expanding field of thermophilicity. Biotechnologists will find the book useful as a source of information on thermophiles or thermostable enzymes of possible industrial use.

Two-Component Signaling Systems, Part C
Two-Component Signaling Systems, Part C

Author:

Publisher: Academic Press

Published: 2010-03-01

Total Pages: 495

ISBN-13: 0123813484

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Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This volume, the third in a three-volume treatment edited by the same group of editors, includes a wide range of methods, including those dealing with the Sln-1 kinase pathway, triazole sensitivity in C. albicans, and histidine kinases in cyanobacteria circadian clock. - Includes time-tested core methods and new innovations applicable to any researcher studing two-component signaling systems or histidine kinases - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Amyloid, Prions, and Other Protein Aggregates, Part C
Amyloid, Prions, and Other Protein Aggregates, Part C

Author:

Publisher: Elsevier

Published: 2006-10-06

Total Pages: 412

ISBN-13: 0080468977

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The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part B (volume 412) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies. - Presents detailed protocols - Includes troubleshooting tips - Provides coverage on structural biology, computational methods, and biology

Cryo-EM, Part C
Cryo-EM, Part C

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Publisher: Academic Press

Published: 2010-09-30

Total Pages: 423

ISBN-13: 0123849942

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This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide

Hydrogen Peroxide and Cell Signaling, Part C
Hydrogen Peroxide and Cell Signaling, Part C

Author:

Publisher: Academic Press

Published: 2013-07-11

Total Pages: 395

ISBN-13: 0124055400

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers hydrogen peroxide and cell signaling - Contains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy